Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05315 and RR42_RS05320 are separated by 170 nucleotides RR42_RS05320 and RR42_RS05325 are separated by 63 nucleotides
RR42_RS05315: RR42_RS05315 - fatty acid desaturase, at 1,174,159 to 1,175,259
_RS05315
RR42_RS05320: RR42_RS05320 - guanine deaminase, at 1,175,430 to 1,176,761
_RS05320
RR42_RS05325: RR42_RS05325 - adenine deaminase, at 1,176,825 to 1,177,871
_RS05325
Position (kb)
1175
1176
1177 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1174.484 kb on + strand, within RR42_RS05315 at 1174.484 kb on + strand, within RR42_RS05315 at 1174.484 kb on + strand, within RR42_RS05315 at 1174.485 kb on - strand, within RR42_RS05315 at 1174.485 kb on - strand, within RR42_RS05315 at 1174.517 kb on + strand, within RR42_RS05315 at 1174.518 kb on - strand, within RR42_RS05315 at 1174.751 kb on + strand, within RR42_RS05315 at 1174.751 kb on + strand, within RR42_RS05315 at 1174.779 kb on - strand, within RR42_RS05315 at 1174.835 kb on + strand, within RR42_RS05315 at 1174.835 kb on + strand, within RR42_RS05315 at 1174.836 kb on - strand, within RR42_RS05315 at 1174.916 kb on + strand, within RR42_RS05315 at 1174.916 kb on + strand, within RR42_RS05315 at 1174.917 kb on - strand, within RR42_RS05315 at 1174.917 kb on - strand, within RR42_RS05315 at 1175.349 kb on + strand at 1175.349 kb on + strand at 1175.350 kb on - strand at 1175.350 kb on - strand at 1175.350 kb on - strand at 1175.476 kb on + strand at 1175.476 kb on + strand at 1175.476 kb on + strand at 1175.476 kb on + strand at 1175.476 kb on + strand at 1175.476 kb on + strand at 1175.476 kb on + strand at 1175.476 kb on + strand at 1175.476 kb on + strand at 1175.476 kb on + strand at 1175.477 kb on - strand at 1175.477 kb on - strand at 1175.681 kb on - strand, within RR42_RS05320 at 1175.681 kb on - strand, within RR42_RS05320 at 1175.833 kb on + strand, within RR42_RS05320 at 1175.926 kb on + strand, within RR42_RS05320 at 1175.927 kb on - strand, within RR42_RS05320 at 1175.941 kb on + strand, within RR42_RS05320 at 1175.977 kb on + strand, within RR42_RS05320 at 1175.977 kb on + strand, within RR42_RS05320 at 1175.977 kb on + strand, within RR42_RS05320 at 1175.977 kb on + strand, within RR42_RS05320 at 1175.978 kb on - strand, within RR42_RS05320 at 1175.978 kb on - strand, within RR42_RS05320 at 1176.144 kb on - strand, within RR42_RS05320 at 1176.151 kb on + strand, within RR42_RS05320 at 1176.410 kb on - strand, within RR42_RS05320 at 1176.446 kb on + strand, within RR42_RS05320 at 1176.446 kb on + strand, within RR42_RS05320 at 1176.447 kb on - strand, within RR42_RS05320 at 1176.447 kb on - strand, within RR42_RS05320 at 1176.447 kb on - strand, within RR42_RS05320 at 1176.447 kb on - strand, within RR42_RS05320 at 1176.447 kb on - strand, within RR42_RS05320 at 1176.447 kb on - strand, within RR42_RS05320 at 1176.448 kb on + strand, within RR42_RS05320 at 1176.448 kb on + strand, within RR42_RS05320 at 1176.448 kb on + strand, within RR42_RS05320 at 1176.448 kb on + strand, within RR42_RS05320 at 1176.448 kb on + strand, within RR42_RS05320 at 1176.449 kb on - strand, within RR42_RS05320 at 1176.449 kb on - strand, within RR42_RS05320 at 1176.449 kb on - strand, within RR42_RS05320 at 1176.449 kb on - strand, within RR42_RS05320 at 1176.449 kb on - strand, within RR42_RS05320 at 1176.449 kb on - strand, within RR42_RS05320 at 1176.662 kb on - strand at 1176.667 kb on + strand at 1176.668 kb on - strand at 1177.004 kb on + strand, within RR42_RS05325 at 1177.004 kb on + strand, within RR42_RS05325 at 1177.005 kb on - strand, within RR42_RS05325 at 1177.005 kb on - strand, within RR42_RS05325 at 1177.100 kb on - strand, within RR42_RS05325 at 1177.475 kb on - strand, within RR42_RS05325 at 1177.547 kb on + strand, within RR42_RS05325 at 1177.548 kb on - strand, within RR42_RS05325 at 1177.613 kb on - strand, within RR42_RS05325 at 1177.613 kb on - strand, within RR42_RS05325 at 1177.670 kb on - strand, within RR42_RS05325 at 1177.672 kb on + strand, within RR42_RS05325 at 1177.672 kb on + strand, within RR42_RS05325 at 1177.672 kb on + strand, within RR42_RS05325 at 1177.672 kb on + strand, within RR42_RS05325 at 1177.673 kb on - strand, within RR42_RS05325 at 1177.673 kb on - strand, within RR42_RS05325 at 1177.708 kb on + strand, within RR42_RS05325 at 1177.709 kb on - strand, within RR42_RS05325 at 1177.738 kb on + strand, within RR42_RS05325 at 1177.743 kb on + strand, within RR42_RS05325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 1,174,484 + RR42_RS05315 0.30 -2.0 1,174,484 + RR42_RS05315 0.30 -0.0 1,174,484 + RR42_RS05315 0.30 +2.3 1,174,485 - RR42_RS05315 0.30 +0.1 1,174,485 - RR42_RS05315 0.30 -2.0 1,174,517 + RR42_RS05315 0.33 +0.4 1,174,518 - RR42_RS05315 0.33 +1.7 1,174,751 + RR42_RS05315 0.54 -0.8 1,174,751 + RR42_RS05315 0.54 +0.1 1,174,779 - RR42_RS05315 0.56 -0.6 1,174,835 + RR42_RS05315 0.61 -2.2 1,174,835 + RR42_RS05315 0.61 -0.3 1,174,836 - RR42_RS05315 0.61 -0.2 1,174,916 + RR42_RS05315 0.69 +2.3 1,174,916 + RR42_RS05315 0.69 -1.0 1,174,917 - RR42_RS05315 0.69 +1.2 1,174,917 - RR42_RS05315 0.69 +1.0 1,175,349 + -0.4 1,175,349 + -0.1 1,175,350 - +0.2 1,175,350 - +0.1 1,175,350 - +1.3 1,175,476 + +0.8 1,175,476 + -1.6 1,175,476 + -2.0 1,175,476 + -0.1 1,175,476 + -0.3 1,175,476 + -0.6 1,175,476 + -1.2 1,175,476 + -0.2 1,175,476 + -0.7 1,175,476 + -0.5 1,175,477 - -0.1 1,175,477 - +2.3 1,175,681 - RR42_RS05320 0.19 -1.6 1,175,681 - RR42_RS05320 0.19 +1.4 1,175,833 + RR42_RS05320 0.30 -1.6 1,175,926 + RR42_RS05320 0.37 -0.9 1,175,927 - RR42_RS05320 0.37 -0.6 1,175,941 + RR42_RS05320 0.38 +0.2 1,175,977 + RR42_RS05320 0.41 -1.4 1,175,977 + RR42_RS05320 0.41 +0.7 1,175,977 + RR42_RS05320 0.41 +0.7 1,175,977 + RR42_RS05320 0.41 -1.1 1,175,978 - RR42_RS05320 0.41 +0.5 1,175,978 - RR42_RS05320 0.41 +0.3 1,176,144 - RR42_RS05320 0.54 -0.9 1,176,151 + RR42_RS05320 0.54 +0.1 1,176,410 - RR42_RS05320 0.74 -1.9 1,176,446 + RR42_RS05320 0.76 -1.1 1,176,446 + RR42_RS05320 0.76 +0.4 1,176,447 - RR42_RS05320 0.76 +1.1 1,176,447 - RR42_RS05320 0.76 -1.7 1,176,447 - RR42_RS05320 0.76 -2.6 1,176,447 - RR42_RS05320 0.76 +0.0 1,176,447 - RR42_RS05320 0.76 -0.2 1,176,447 - RR42_RS05320 0.76 +0.3 1,176,448 + RR42_RS05320 0.76 +2.3 1,176,448 + RR42_RS05320 0.76 -0.1 1,176,448 + RR42_RS05320 0.76 -1.6 1,176,448 + RR42_RS05320 0.76 +1.3 1,176,448 + RR42_RS05320 0.76 -1.7 1,176,449 - RR42_RS05320 0.77 +1.3 1,176,449 - RR42_RS05320 0.77 -0.4 1,176,449 - RR42_RS05320 0.77 -0.3 1,176,449 - RR42_RS05320 0.77 +0.2 1,176,449 - RR42_RS05320 0.77 +0.2 1,176,449 - RR42_RS05320 0.77 -0.8 1,176,662 - -1.5 1,176,667 + -1.2 1,176,668 - +1.3 1,177,004 + RR42_RS05325 0.17 -0.4 1,177,004 + RR42_RS05325 0.17 +1.3 1,177,005 - RR42_RS05325 0.17 +4.3 1,177,005 - RR42_RS05325 0.17 +0.1 1,177,100 - RR42_RS05325 0.26 +0.3 1,177,475 - RR42_RS05325 0.62 -0.1 1,177,547 + RR42_RS05325 0.69 -0.4 1,177,548 - RR42_RS05325 0.69 -0.5 1,177,613 - RR42_RS05325 0.75 -0.7 1,177,613 - RR42_RS05325 0.75 +0.0 1,177,670 - RR42_RS05325 0.81 +1.3 1,177,672 + RR42_RS05325 0.81 -1.2 1,177,672 + RR42_RS05325 0.81 -1.7 1,177,672 + RR42_RS05325 0.81 -1.7 1,177,672 + RR42_RS05325 0.81 -0.6 1,177,673 - RR42_RS05325 0.81 +0.6 1,177,673 - RR42_RS05325 0.81 -1.6 1,177,708 + RR42_RS05325 0.84 -0.5 1,177,709 - RR42_RS05325 0.84 +0.1 1,177,738 + RR42_RS05325 0.87 -1.3 1,177,743 + RR42_RS05325 0.88 +2.3
Or see this region's nucleotide sequence