Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05120 and RR42_RS05125 are separated by 189 nucleotides RR42_RS05125 and RR42_RS05130 overlap by 4 nucleotides RR42_RS05130 and RR42_RS05135 overlap by 4 nucleotides
RR42_RS05120: RR42_RS05120 - ABC transporter substrate-binding protein, at 1,130,304 to 1,131,284
_RS05120
RR42_RS05125: RR42_RS05125 - malonic semialdehyde reductase, at 1,131,474 to 1,132,058
_RS05125
RR42_RS05130: RR42_RS05130 - 2-aminomuconate deaminase, at 1,132,055 to 1,132,468
_RS05130
RR42_RS05135: RR42_RS05135 - dihydrodipicolinate synthetase, at 1,132,465 to 1,133,379
_RS05135
Position (kb) 1131
1132
1133 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1130.528 kb on + strand, within RR42_RS05120 at 1130.548 kb on + strand, within RR42_RS05120 at 1130.668 kb on + strand, within RR42_RS05120 at 1130.809 kb on - strand, within RR42_RS05120 at 1131.010 kb on + strand, within RR42_RS05120 at 1131.011 kb on - strand, within RR42_RS05120 at 1131.038 kb on - strand, within RR42_RS05120 at 1131.674 kb on - strand, within RR42_RS05125 at 1131.746 kb on - strand, within RR42_RS05125 at 1131.959 kb on - strand, within RR42_RS05125 at 1131.959 kb on - strand, within RR42_RS05125 at 1132.128 kb on - strand, within RR42_RS05130 at 1132.218 kb on + strand, within RR42_RS05130 at 1132.218 kb on + strand, within RR42_RS05130 at 1132.218 kb on + strand, within RR42_RS05130 at 1132.219 kb on - strand, within RR42_RS05130 at 1132.703 kb on + strand, within RR42_RS05135 at 1132.704 kb on - strand, within RR42_RS05135 at 1132.716 kb on + strand, within RR42_RS05135 at 1132.961 kb on + strand, within RR42_RS05135 at 1133.015 kb on + strand, within RR42_RS05135 at 1133.016 kb on - strand, within RR42_RS05135
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 1,130,528 + RR42_RS05120 0.23 -0.1 1,130,548 + RR42_RS05120 0.25 +0.0 1,130,668 + RR42_RS05120 0.37 +3.1 1,130,809 - RR42_RS05120 0.51 -0.4 1,131,010 + RR42_RS05120 0.72 +1.7 1,131,011 - RR42_RS05120 0.72 +0.2 1,131,038 - RR42_RS05120 0.75 +1.9 1,131,674 - RR42_RS05125 0.34 +2.3 1,131,746 - RR42_RS05125 0.46 +0.0 1,131,959 - RR42_RS05125 0.83 +0.7 1,131,959 - RR42_RS05125 0.83 +1.7 1,132,128 - RR42_RS05130 0.18 +2.3 1,132,218 + RR42_RS05130 0.39 +0.5 1,132,218 + RR42_RS05130 0.39 +0.5 1,132,218 + RR42_RS05130 0.39 +0.2 1,132,219 - RR42_RS05130 0.40 -0.5 1,132,703 + RR42_RS05135 0.26 +0.2 1,132,704 - RR42_RS05135 0.26 +0.3 1,132,716 + RR42_RS05135 0.27 -1.9 1,132,961 + RR42_RS05135 0.54 +0.0 1,133,015 + RR42_RS05135 0.60 -0.3 1,133,016 - RR42_RS05135 0.60 -0.5
Or see this region's nucleotide sequence