Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03665 and RR42_RS03670 are separated by 74 nucleotides RR42_RS03670 and RR42_RS03675 are separated by 594 nucleotides RR42_RS03675 and RR42_RS03680 are separated by 27 nucleotides RR42_RS03680 and RR42_RS03685 are separated by 110 nucleotides
RR42_RS03665: RR42_RS03665 - translation initiation factor IF-1, at 812,323 to 812,544
_RS03665
RR42_RS03670: RR42_RS03670 - isochorismatase, at 812,619 to 812,813
_RS03670
RR42_RS03675: RR42_RS03675 - hypothetical protein, at 813,408 to 813,608
_RS03675
RR42_RS03680: RR42_RS03680 - hypothetical protein, at 813,636 to 813,878
_RS03680
RR42_RS03685: RR42_RS03685 - GTP cyclohydrolase, at 813,989 to 814,258
_RS03685
Position (kb)
813
814 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 812.441 kb on + strand, within RR42_RS03665 at 812.498 kb on - strand, within RR42_RS03665 at 812.527 kb on + strand at 812.539 kb on - strand at 812.676 kb on - strand, within RR42_RS03670 at 812.764 kb on + strand, within RR42_RS03670 at 812.765 kb on - strand, within RR42_RS03670 at 812.796 kb on + strand at 812.797 kb on - strand at 812.818 kb on + strand at 812.818 kb on + strand at 812.850 kb on - strand at 812.858 kb on + strand at 812.859 kb on - strand at 813.017 kb on + strand at 813.017 kb on + strand at 813.063 kb on + strand at 813.064 kb on - strand at 813.167 kb on + strand at 813.168 kb on - strand at 813.244 kb on + strand at 813.244 kb on + strand at 813.286 kb on + strand at 813.286 kb on + strand at 813.287 kb on - strand at 813.295 kb on + strand at 813.295 kb on + strand at 813.296 kb on - strand at 813.296 kb on - strand at 813.296 kb on - strand at 813.438 kb on + strand, within RR42_RS03675 at 813.439 kb on - strand, within RR42_RS03675 at 813.439 kb on - strand, within RR42_RS03675 at 813.490 kb on - strand, within RR42_RS03675 at 813.490 kb on - strand, within RR42_RS03675 at 813.490 kb on - strand, within RR42_RS03675 at 813.492 kb on - strand, within RR42_RS03675 at 813.492 kb on - strand, within RR42_RS03675 at 813.500 kb on + strand, within RR42_RS03675 at 813.638 kb on - strand at 813.661 kb on + strand, within RR42_RS03680 at 813.661 kb on + strand, within RR42_RS03680 at 813.662 kb on - strand, within RR42_RS03680 at 813.662 kb on - strand, within RR42_RS03680 at 813.990 kb on + strand at 813.990 kb on + strand at 814.039 kb on - strand, within RR42_RS03685 at 814.208 kb on + strand, within RR42_RS03685 at 814.284 kb on + strand at 814.284 kb on + strand at 814.285 kb on - strand at 814.398 kb on + strand at 814.427 kb on - strand at 814.499 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 812,441 + RR42_RS03665 0.53 +0.3 812,498 - RR42_RS03665 0.79 +0.6 812,527 + -1.4 812,539 - +1.4 812,676 - RR42_RS03670 0.29 -0.4 812,764 + RR42_RS03670 0.74 +0.8 812,765 - RR42_RS03670 0.75 +1.2 812,796 + +2.0 812,797 - -0.4 812,818 + +0.3 812,818 + +1.1 812,850 - -2.0 812,858 + -0.8 812,859 - -1.2 813,017 + -0.4 813,017 + +0.8 813,063 + +1.7 813,064 - +1.8 813,167 + -0.2 813,168 - -1.4 813,244 + -1.6 813,244 + -1.7 813,286 + +0.6 813,286 + -0.7 813,287 - -0.3 813,295 + -0.8 813,295 + -1.2 813,296 - -0.6 813,296 - -0.2 813,296 - -0.3 813,438 + RR42_RS03675 0.15 -1.8 813,439 - RR42_RS03675 0.15 -0.5 813,439 - RR42_RS03675 0.15 -1.4 813,490 - RR42_RS03675 0.41 +0.4 813,490 - RR42_RS03675 0.41 +0.2 813,490 - RR42_RS03675 0.41 -0.6 813,492 - RR42_RS03675 0.42 +0.4 813,492 - RR42_RS03675 0.42 -0.7 813,500 + RR42_RS03675 0.46 -0.8 813,638 - -0.9 813,661 + RR42_RS03680 0.10 +0.0 813,661 + RR42_RS03680 0.10 -0.8 813,662 - RR42_RS03680 0.11 +1.1 813,662 - RR42_RS03680 0.11 +1.4 813,990 + +1.3 813,990 + +1.0 814,039 - RR42_RS03685 0.19 +0.6 814,208 + RR42_RS03685 0.81 -1.3 814,284 + +0.8 814,284 + +0.1 814,285 - +0.8 814,398 + +1.7 814,427 - -0.2 814,499 - -0.1
Or see this region's nucleotide sequence