Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03270 and RR42_RS03275 are separated by 94 nucleotides RR42_RS03275 and RR42_RS03280 are separated by 117 nucleotides RR42_RS03280 and RR42_RS03285 are separated by 57 nucleotides
RR42_RS03270: RR42_RS03270 - membrane protein, at 724,868 to 725,077
_RS03270
RR42_RS03275: RR42_RS03275 - membrane protein, at 725,172 to 725,642
_RS03275
RR42_RS03280: RR42_RS03280 - LysR family transcriptional regulator, at 725,760 to 726,665
_RS03280
RR42_RS03285: RR42_RS03285 - tricarboxylic transport membrane protein, at 726,723 to 727,679
_RS03285
Position (kb) 725
726
727 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 724.764 kb on + strand at 724.764 kb on + strand at 724.765 kb on - strand at 724.765 kb on - strand at 724.765 kb on - strand at 724.765 kb on - strand at 724.874 kb on - strand at 724.879 kb on - strand at 724.888 kb on + strand at 724.888 kb on + strand at 725.166 kb on + strand at 725.166 kb on + strand at 725.167 kb on - strand at 725.167 kb on - strand at 725.167 kb on - strand at 725.167 kb on - strand at 725.187 kb on + strand at 725.281 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.291 kb on - strand, within RR42_RS03275 at 725.291 kb on - strand, within RR42_RS03275 at 725.291 kb on - strand, within RR42_RS03275 at 725.479 kb on + strand, within RR42_RS03275 at 725.480 kb on - strand, within RR42_RS03275 at 725.612 kb on + strand at 725.613 kb on - strand at 725.667 kb on + strand at 725.667 kb on + strand at 725.667 kb on + strand at 725.667 kb on + strand at 725.668 kb on - strand at 725.700 kb on - strand at 726.067 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.663 kb on + strand at 726.664 kb on - strand at 726.698 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.788 kb on + strand at 726.788 kb on + strand at 726.898 kb on + strand, within RR42_RS03285 at 726.899 kb on - strand, within RR42_RS03285 at 726.899 kb on - strand, within RR42_RS03285 at 726.928 kb on + strand, within RR42_RS03285 at 726.929 kb on - strand, within RR42_RS03285 at 726.929 kb on - strand, within RR42_RS03285 at 727.165 kb on + strand, within RR42_RS03285 at 727.165 kb on + strand, within RR42_RS03285 at 727.166 kb on - strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.320 kb on + strand, within RR42_RS03285 at 727.331 kb on - strand, within RR42_RS03285 at 727.375 kb on + strand, within RR42_RS03285 at 727.376 kb on - strand, within RR42_RS03285 at 727.376 kb on - strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.475 kb on - strand, within RR42_RS03285 at 727.505 kb on - strand, within RR42_RS03285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 724,764 + +1.9 724,764 + -0.2 724,765 - -1.2 724,765 - +0.2 724,765 - -0.3 724,765 - -1.0 724,874 - -0.1 724,879 - -1.0 724,888 + +0.1 724,888 + +0.1 725,166 + +0.7 725,166 + -1.7 725,167 - -1.6 725,167 - -0.6 725,167 - +0.1 725,167 - +0.1 725,187 + -0.1 725,281 + RR42_RS03275 0.23 +4.9 725,290 + RR42_RS03275 0.25 +2.8 725,290 + RR42_RS03275 0.25 +2.9 725,290 + RR42_RS03275 0.25 +3.8 725,290 + RR42_RS03275 0.25 +3.2 725,290 + RR42_RS03275 0.25 +4.0 725,290 + RR42_RS03275 0.25 +3.2 725,291 - RR42_RS03275 0.25 +5.3 725,291 - RR42_RS03275 0.25 +3.5 725,291 - RR42_RS03275 0.25 +3.7 725,479 + RR42_RS03275 0.65 +4.1 725,480 - RR42_RS03275 0.65 +1.9 725,612 + +3.9 725,613 - +3.2 725,667 + +3.2 725,667 + +2.6 725,667 + +2.5 725,667 + +3.4 725,668 - -1.1 725,700 - -1.8 726,067 - RR42_RS03280 0.34 -1.3 726,194 - RR42_RS03280 0.48 +0.1 726,194 - RR42_RS03280 0.48 -0.6 726,194 - RR42_RS03280 0.48 -1.5 726,194 - RR42_RS03280 0.48 -2.1 726,663 + -0.3 726,664 - +0.8 726,698 + +1.3 726,784 + +0.5 726,784 + -0.3 726,784 + -1.2 726,784 + -0.7 726,784 + -0.2 726,784 + -1.5 726,784 + +0.3 726,784 + +0.0 726,784 + -1.6 726,784 + -0.4 726,784 + +0.4 726,785 - +0.5 726,785 - -0.3 726,785 - -0.2 726,785 - -0.7 726,785 - +0.9 726,785 - +0.5 726,785 - -1.9 726,785 - -0.7 726,785 - +1.5 726,785 - +0.8 726,788 + +2.1 726,788 + -0.3 726,898 + RR42_RS03285 0.18 +0.9 726,899 - RR42_RS03285 0.18 +1.3 726,899 - RR42_RS03285 0.18 -1.7 726,928 + RR42_RS03285 0.21 +0.4 726,929 - RR42_RS03285 0.22 -0.6 726,929 - RR42_RS03285 0.22 +0.0 727,165 + RR42_RS03285 0.46 +0.6 727,165 + RR42_RS03285 0.46 -0.5 727,166 - RR42_RS03285 0.46 +1.9 727,245 + RR42_RS03285 0.55 +1.3 727,245 + RR42_RS03285 0.55 -0.7 727,245 + RR42_RS03285 0.55 +0.1 727,245 + RR42_RS03285 0.55 +0.9 727,246 - RR42_RS03285 0.55 +0.7 727,246 - RR42_RS03285 0.55 -1.2 727,246 - RR42_RS03285 0.55 +0.2 727,320 + RR42_RS03285 0.62 +0.6 727,331 - RR42_RS03285 0.64 -0.6 727,375 + RR42_RS03285 0.68 -0.1 727,376 - RR42_RS03285 0.68 +1.1 727,376 - RR42_RS03285 0.68 +0.5 727,474 + RR42_RS03285 0.78 -0.8 727,474 + RR42_RS03285 0.78 -1.3 727,474 + RR42_RS03285 0.78 -0.5 727,474 + RR42_RS03285 0.78 -0.4 727,475 - RR42_RS03285 0.79 -0.6 727,505 - RR42_RS03285 0.82 -0.7
Or see this region's nucleotide sequence