Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03180 and RR42_RS03185 are separated by 153 nucleotides RR42_RS03185 and RR42_RS03190 overlap by 4 nucleotides RR42_RS03190 and RR42_RS03195 are separated by 40 nucleotides
RR42_RS03180: RR42_RS03180 - ABC transporter substrate-binding protein, at 704,625 to 705,665
_RS03180
RR42_RS03185: RR42_RS03185 - branched-chain amino acid permease, at 705,819 to 706,649
_RS03185
RR42_RS03190: RR42_RS03190 - branched-chain amino acid transporter, at 706,646 to 706,969
_RS03190
RR42_RS03195: RR42_RS03195 - LuxR family transcriptional regulator, at 707,010 to 708,137
_RS03195
Position (kb)
705
706
707 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 704.880 kb on + strand, within RR42_RS03180 at 704.880 kb on + strand, within RR42_RS03180 at 704.880 kb on + strand, within RR42_RS03180 at 704.880 kb on + strand, within RR42_RS03180 at 704.881 kb on - strand, within RR42_RS03180 at 704.881 kb on - strand, within RR42_RS03180 at 704.881 kb on - strand, within RR42_RS03180 at 704.881 kb on - strand, within RR42_RS03180 at 705.000 kb on + strand, within RR42_RS03180 at 705.000 kb on + strand, within RR42_RS03180 at 705.150 kb on + strand, within RR42_RS03180 at 705.151 kb on - strand, within RR42_RS03180 at 705.151 kb on - strand, within RR42_RS03180 at 705.156 kb on + strand, within RR42_RS03180 at 705.156 kb on + strand, within RR42_RS03180 at 705.157 kb on - strand, within RR42_RS03180 at 705.157 kb on - strand, within RR42_RS03180 at 705.157 kb on - strand, within RR42_RS03180 at 705.157 kb on - strand, within RR42_RS03180 at 705.227 kb on + strand, within RR42_RS03180 at 705.227 kb on + strand, within RR42_RS03180 at 705.227 kb on + strand, within RR42_RS03180 at 705.227 kb on + strand, within RR42_RS03180 at 705.243 kb on + strand, within RR42_RS03180 at 705.243 kb on + strand, within RR42_RS03180 at 705.243 kb on + strand, within RR42_RS03180 at 705.330 kb on + strand, within RR42_RS03180 at 705.331 kb on - strand, within RR42_RS03180 at 705.656 kb on + strand at 705.656 kb on + strand at 705.656 kb on + strand at 705.657 kb on - strand at 705.657 kb on - strand at 705.666 kb on + strand at 705.666 kb on + strand at 705.666 kb on + strand at 705.667 kb on - strand at 705.688 kb on + strand at 705.688 kb on + strand at 705.688 kb on + strand at 705.688 kb on + strand at 705.689 kb on - strand at 705.689 kb on - strand at 705.695 kb on - strand at 705.695 kb on - strand at 705.718 kb on + strand at 705.730 kb on + strand at 705.730 kb on + strand at 705.730 kb on + strand at 705.730 kb on + strand at 705.731 kb on - strand at 705.752 kb on + strand at 705.753 kb on - strand at 705.753 kb on - strand at 705.753 kb on - strand at 705.753 kb on - strand at 705.753 kb on - strand at 705.754 kb on + strand at 705.754 kb on + strand at 705.754 kb on + strand at 705.755 kb on - strand at 705.755 kb on - strand at 705.755 kb on - strand at 705.818 kb on + strand at 705.890 kb on + strand at 705.891 kb on - strand at 705.891 kb on - strand at 705.907 kb on - strand, within RR42_RS03185 at 706.018 kb on - strand, within RR42_RS03185 at 706.227 kb on + strand, within RR42_RS03185 at 706.263 kb on + strand, within RR42_RS03185 at 706.772 kb on + strand, within RR42_RS03190 at 706.856 kb on + strand, within RR42_RS03190 at 706.856 kb on + strand, within RR42_RS03190 at 706.856 kb on + strand, within RR42_RS03190 at 706.856 kb on + strand, within RR42_RS03190 at 706.856 kb on + strand, within RR42_RS03190 at 706.856 kb on + strand, within RR42_RS03190 at 706.857 kb on - strand, within RR42_RS03190 at 706.857 kb on - strand, within RR42_RS03190 at 706.857 kb on - strand, within RR42_RS03190 at 706.857 kb on - strand, within RR42_RS03190 at 706.857 kb on - strand, within RR42_RS03190 at 706.893 kb on - strand, within RR42_RS03190 at 706.943 kb on + strand at 706.943 kb on + strand at 706.943 kb on + strand at 706.943 kb on + strand at 707.236 kb on + strand, within RR42_RS03195 at 707.237 kb on - strand, within RR42_RS03195 at 707.237 kb on - strand, within RR42_RS03195
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 704,880 + RR42_RS03180 0.24 +0.1 704,880 + RR42_RS03180 0.24 -0.7 704,880 + RR42_RS03180 0.24 +1.0 704,880 + RR42_RS03180 0.24 -0.2 704,881 - RR42_RS03180 0.25 +2.5 704,881 - RR42_RS03180 0.25 -0.3 704,881 - RR42_RS03180 0.25 +0.6 704,881 - RR42_RS03180 0.25 -0.3 705,000 + RR42_RS03180 0.36 -0.1 705,000 + RR42_RS03180 0.36 +0.0 705,150 + RR42_RS03180 0.50 +0.1 705,151 - RR42_RS03180 0.51 +0.2 705,151 - RR42_RS03180 0.51 +0.1 705,156 + RR42_RS03180 0.51 -0.8 705,156 + RR42_RS03180 0.51 -0.0 705,157 - RR42_RS03180 0.51 +0.7 705,157 - RR42_RS03180 0.51 -0.7 705,157 - RR42_RS03180 0.51 +0.5 705,157 - RR42_RS03180 0.51 -0.9 705,227 + RR42_RS03180 0.58 +0.4 705,227 + RR42_RS03180 0.58 +1.4 705,227 + RR42_RS03180 0.58 -0.1 705,227 + RR42_RS03180 0.58 -0.1 705,243 + RR42_RS03180 0.59 +1.5 705,243 + RR42_RS03180 0.59 +0.4 705,243 + RR42_RS03180 0.59 +0.1 705,330 + RR42_RS03180 0.68 +0.0 705,331 - RR42_RS03180 0.68 -0.9 705,656 + -0.8 705,656 + -1.6 705,656 + -0.5 705,657 - +0.1 705,657 - -0.0 705,666 + -1.1 705,666 + -1.1 705,666 + -0.9 705,667 - -0.9 705,688 + -1.2 705,688 + -0.5 705,688 + -0.2 705,688 + -0.9 705,689 - -1.1 705,689 - -1.0 705,695 - -2.6 705,695 - +0.4 705,718 + -2.3 705,730 + +0.3 705,730 + +0.1 705,730 + -0.3 705,730 + -0.3 705,731 - +0.1 705,752 + -2.2 705,753 - +0.7 705,753 - +0.3 705,753 - -0.4 705,753 - +0.3 705,753 - -0.5 705,754 + -1.1 705,754 + +0.2 705,754 + +0.9 705,755 - +2.5 705,755 - -0.1 705,755 - -0.1 705,818 + -2.8 705,890 + -2.1 705,891 - +1.1 705,891 - +2.5 705,907 - RR42_RS03185 0.11 -2.2 706,018 - RR42_RS03185 0.24 -0.2 706,227 + RR42_RS03185 0.49 -1.8 706,263 + RR42_RS03185 0.53 +0.1 706,772 + RR42_RS03190 0.39 -0.5 706,856 + RR42_RS03190 0.65 +0.4 706,856 + RR42_RS03190 0.65 +1.0 706,856 + RR42_RS03190 0.65 +0.4 706,856 + RR42_RS03190 0.65 +0.4 706,856 + RR42_RS03190 0.65 -0.1 706,856 + RR42_RS03190 0.65 -0.5 706,857 - RR42_RS03190 0.65 +0.2 706,857 - RR42_RS03190 0.65 +0.1 706,857 - RR42_RS03190 0.65 -0.4 706,857 - RR42_RS03190 0.65 -0.6 706,857 - RR42_RS03190 0.65 -0.2 706,893 - RR42_RS03190 0.76 +0.1 706,943 + +0.5 706,943 + +0.9 706,943 + -0.2 706,943 + -0.5 707,236 + RR42_RS03195 0.20 +0.9 707,237 - RR42_RS03195 0.20 -0.4 707,237 - RR42_RS03195 0.20 +0.2
Or see this region's nucleotide sequence