Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03105 and RR42_RS03110 are separated by 380 nucleotides RR42_RS03110 and RR42_RS03115 are separated by 102 nucleotides
RR42_RS03105: RR42_RS03105 - integral membrane protein, at 686,610 to 687,305
_RS03105
RR42_RS03110: RR42_RS03110 - pilus assembly protein TapA, at 687,686 to 688,144
_RS03110
RR42_RS03115: RR42_RS03115 - ligase, at 688,247 to 690,127
_RS03115
Position (kb)
687
688
689 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 686.826 kb on + strand, within RR42_RS03105 at 686.827 kb on - strand, within RR42_RS03105 at 686.859 kb on + strand, within RR42_RS03105 at 686.859 kb on + strand, within RR42_RS03105 at 686.860 kb on - strand, within RR42_RS03105 at 686.943 kb on - strand, within RR42_RS03105 at 686.943 kb on - strand, within RR42_RS03105 at 687.088 kb on + strand, within RR42_RS03105 at 687.088 kb on + strand, within RR42_RS03105 at 687.088 kb on + strand, within RR42_RS03105 at 687.089 kb on - strand, within RR42_RS03105 at 687.151 kb on - strand, within RR42_RS03105 at 687.151 kb on - strand, within RR42_RS03105 at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.316 kb on - strand at 687.316 kb on - strand at 687.392 kb on + strand at 687.393 kb on - strand at 687.393 kb on - strand at 687.461 kb on - strand at 687.461 kb on - strand at 687.465 kb on + strand at 687.484 kb on + strand at 687.484 kb on + strand at 687.484 kb on + strand at 687.485 kb on - strand at 687.485 kb on - strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.489 kb on - strand at 687.489 kb on - strand at 687.489 kb on - strand at 687.588 kb on + strand at 687.595 kb on + strand at 687.595 kb on + strand at 687.596 kb on - strand at 687.623 kb on + strand at 687.623 kb on + strand at 687.797 kb on + strand, within RR42_RS03110 at 687.797 kb on + strand, within RR42_RS03110 at 687.797 kb on + strand, within RR42_RS03110 at 687.798 kb on - strand, within RR42_RS03110 at 687.838 kb on - strand, within RR42_RS03110 at 687.898 kb on + strand, within RR42_RS03110 at 687.899 kb on - strand, within RR42_RS03110 at 687.941 kb on - strand, within RR42_RS03110 at 688.072 kb on + strand, within RR42_RS03110 at 688.072 kb on + strand, within RR42_RS03110 at 688.073 kb on - strand, within RR42_RS03110 at 688.146 kb on + strand at 688.147 kb on - strand at 688.150 kb on + strand at 688.150 kb on + strand at 688.150 kb on + strand at 688.150 kb on + strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.204 kb on + strand at 688.204 kb on + strand at 688.204 kb on + strand at 688.204 kb on + strand at 688.205 kb on - strand at 688.205 kb on - strand at 688.205 kb on - strand at 688.231 kb on + strand at 688.231 kb on + strand at 688.232 kb on - strand at 688.336 kb on - strand at 688.346 kb on + strand at 688.347 kb on - strand at 688.347 kb on - strand at 688.347 kb on - strand at 688.347 kb on - strand at 688.433 kb on + strand at 688.707 kb on + strand, within RR42_RS03115 at 688.755 kb on - strand, within RR42_RS03115 at 688.755 kb on - strand, within RR42_RS03115 at 688.964 kb on + strand, within RR42_RS03115 at 688.964 kb on + strand, within RR42_RS03115 at 688.965 kb on - strand, within RR42_RS03115 at 688.965 kb on - strand, within RR42_RS03115 at 688.965 kb on - strand, within RR42_RS03115
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 686,826 + RR42_RS03105 0.31 +1.2 686,827 - RR42_RS03105 0.31 -0.3 686,859 + RR42_RS03105 0.36 -0.0 686,859 + RR42_RS03105 0.36 +0.2 686,860 - RR42_RS03105 0.36 +0.5 686,943 - RR42_RS03105 0.48 +0.7 686,943 - RR42_RS03105 0.48 +0.3 687,088 + RR42_RS03105 0.69 -1.1 687,088 + RR42_RS03105 0.69 -0.5 687,088 + RR42_RS03105 0.69 -0.7 687,089 - RR42_RS03105 0.69 +0.9 687,151 - RR42_RS03105 0.78 +1.2 687,151 - RR42_RS03105 0.78 -2.7 687,315 + -0.0 687,315 + -1.2 687,315 + +1.9 687,315 + -0.4 687,315 + +1.1 687,315 + +0.4 687,316 - +1.6 687,316 - +0.2 687,392 + -0.8 687,393 - -0.4 687,393 - -0.2 687,461 - +0.7 687,461 - -1.6 687,465 + -0.5 687,484 + -0.5 687,484 + -0.7 687,484 + +0.3 687,485 - -0.7 687,485 - +1.2 687,488 + -1.3 687,488 + -1.5 687,488 + -0.1 687,488 + -1.1 687,488 + -0.3 687,488 + -0.2 687,488 + +0.1 687,488 + +1.7 687,488 + -1.1 687,488 + +0.5 687,489 - -0.3 687,489 - -2.7 687,489 - +2.9 687,588 + -0.5 687,595 + -0.4 687,595 + -0.4 687,596 - +1.2 687,623 + +0.2 687,623 + -0.5 687,797 + RR42_RS03110 0.24 -0.0 687,797 + RR42_RS03110 0.24 -0.0 687,797 + RR42_RS03110 0.24 +1.1 687,798 - RR42_RS03110 0.24 -0.3 687,838 - RR42_RS03110 0.33 +1.2 687,898 + RR42_RS03110 0.46 -0.1 687,899 - RR42_RS03110 0.46 +1.7 687,941 - RR42_RS03110 0.56 +0.7 688,072 + RR42_RS03110 0.84 +0.5 688,072 + RR42_RS03110 0.84 +1.7 688,073 - RR42_RS03110 0.84 +0.1 688,146 + -0.8 688,147 - -0.5 688,150 + -1.1 688,150 + -2.1 688,150 + -1.0 688,150 + +1.6 688,151 - +0.4 688,151 - -0.1 688,151 - -0.3 688,151 - -1.8 688,151 - -0.3 688,151 - -1.5 688,151 - -0.3 688,151 - -0.3 688,204 + +0.2 688,204 + +0.7 688,204 + -0.1 688,204 + -1.9 688,205 - -2.2 688,205 - -1.3 688,205 - -0.7 688,231 + -0.2 688,231 + +0.6 688,232 - +0.7 688,336 - +0.9 688,346 + -1.5 688,347 - +0.9 688,347 - +0.5 688,347 - +0.2 688,347 - +0.8 688,433 + +0.2 688,707 + RR42_RS03115 0.24 +0.7 688,755 - RR42_RS03115 0.27 -2.4 688,755 - RR42_RS03115 0.27 +0.8 688,964 + RR42_RS03115 0.38 -1.3 688,964 + RR42_RS03115 0.38 -0.2 688,965 - RR42_RS03115 0.38 -0.5 688,965 - RR42_RS03115 0.38 -1.3 688,965 - RR42_RS03115 0.38 +0.1
Or see this region's nucleotide sequence