Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03025 and RR42_RS03030 are separated by 89 nucleotides RR42_RS03030 and RR42_RS03035 overlap by 4 nucleotides RR42_RS03035 and RR42_RS03040 overlap by 4 nucleotides
RR42_RS03025: RR42_RS03025 - Inner membrane protein (FUSC-like) required for 4-hydroxybenzoate transport, together with NodT, MFP, and DUF1656 proteins (RR42_RS03040, RR42_RS03035, and RR42_RS03030) (from data) , at 669,371 to 671,365
_RS03025
RR42_RS03030: RR42_RS03030 - required for 4-hydroxybenzoate transport, together with NodT, MFP, and FUSC proteins (RR42_RS03040, RR42_RS03035, and RR42_RS03025) (from data) , at 671,455 to 671,664
_RS03030
RR42_RS03035: RR42_RS03035 - membrane protein, at 671,661 to 672,629
_RS03035
RR42_RS03040: RR42_RS03040 - Outer membrane protein (NodT-like) required for 4-hydroxybenzoate transport, together with FUSC, MFP, and DUF1656 proteins (RR42_RS03025, RR42_RS03035, and RR42_RS03030) (from data) , at 672,626 to 674,089
_RS03040
Position (kb)
671
672 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 670.668 kb on - strand, within RR42_RS03025 at 670.668 kb on - strand, within RR42_RS03025 at 670.714 kb on - strand, within RR42_RS03025 at 670.714 kb on - strand, within RR42_RS03025 at 670.733 kb on + strand, within RR42_RS03025 at 670.833 kb on - strand, within RR42_RS03025 at 671.213 kb on + strand at 671.216 kb on + strand at 671.217 kb on - strand at 671.217 kb on - strand at 671.217 kb on - strand at 671.476 kb on + strand, within RR42_RS03030 at 671.476 kb on + strand, within RR42_RS03030 at 671.476 kb on + strand, within RR42_RS03030 at 671.476 kb on + strand, within RR42_RS03030 at 671.477 kb on - strand, within RR42_RS03030 at 671.477 kb on - strand, within RR42_RS03030 at 671.609 kb on + strand, within RR42_RS03030 at 671.609 kb on + strand, within RR42_RS03030 at 671.610 kb on - strand, within RR42_RS03030 at 671.610 kb on - strand, within RR42_RS03030 at 671.610 kb on - strand, within RR42_RS03030 at 671.610 kb on - strand, within RR42_RS03030 at 671.611 kb on + strand, within RR42_RS03030 at 671.611 kb on + strand, within RR42_RS03030 at 671.611 kb on + strand, within RR42_RS03030 at 671.611 kb on + strand, within RR42_RS03030 at 671.611 kb on + strand, within RR42_RS03030 at 671.612 kb on - strand, within RR42_RS03030 at 671.612 kb on - strand, within RR42_RS03030 at 671.639 kb on - strand, within RR42_RS03030 at 671.639 kb on - strand, within RR42_RS03030 at 671.678 kb on + strand at 671.699 kb on + strand at 671.752 kb on - strand at 671.754 kb on + strand at 671.754 kb on + strand at 672.147 kb on + strand, within RR42_RS03035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 670,668 - RR42_RS03025 0.65 +0.1 670,668 - RR42_RS03025 0.65 +0.1 670,714 - RR42_RS03025 0.67 +1.1 670,714 - RR42_RS03025 0.67 +0.3 670,733 + RR42_RS03025 0.68 -0.8 670,833 - RR42_RS03025 0.73 +0.9 671,213 + -0.1 671,216 + +0.7 671,217 - -1.2 671,217 - -0.8 671,217 - -1.6 671,476 + RR42_RS03030 0.10 -0.4 671,476 + RR42_RS03030 0.10 -0.3 671,476 + RR42_RS03030 0.10 -0.6 671,476 + RR42_RS03030 0.10 +2.5 671,477 - RR42_RS03030 0.10 -0.3 671,477 - RR42_RS03030 0.10 -1.2 671,609 + RR42_RS03030 0.73 -0.5 671,609 + RR42_RS03030 0.73 +0.5 671,610 - RR42_RS03030 0.74 -0.2 671,610 - RR42_RS03030 0.74 +1.3 671,610 - RR42_RS03030 0.74 -1.1 671,610 - RR42_RS03030 0.74 -0.2 671,611 + RR42_RS03030 0.74 +0.9 671,611 + RR42_RS03030 0.74 -1.3 671,611 + RR42_RS03030 0.74 -1.0 671,611 + RR42_RS03030 0.74 +0.0 671,611 + RR42_RS03030 0.74 +0.2 671,612 - RR42_RS03030 0.75 -1.3 671,612 - RR42_RS03030 0.75 -1.3 671,639 - RR42_RS03030 0.88 -2.3 671,639 - RR42_RS03030 0.88 +0.2 671,678 + -0.2 671,699 + +0.3 671,752 - -0.7 671,754 + +0.8 671,754 + +1.1 672,147 + RR42_RS03035 0.50 -1.4
Or see this region's nucleotide sequence