Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02025 and RR42_RS02030 overlap by 4 nucleotides RR42_RS02030 and RR42_RS02035 are separated by 119 nucleotides RR42_RS02035 and RR42_RS02040 are separated by 140 nucleotides
RR42_RS02025: RR42_RS02025 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 450,651 to 451,529
_RS02025
RR42_RS02030: RR42_RS02030 - membrane protein, at 451,526 to 452,146
_RS02030
RR42_RS02035: RR42_RS02035 - hypothetical protein, at 452,266 to 454,149
_RS02035
RR42_RS02040: RR42_RS02040 - formamidopyrimidine-DNA glycosylase, at 454,290 to 455,171
_RS02040
Position (kb)
452
453
454
455 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 451.820 kb on - strand, within RR42_RS02030 at 452.002 kb on - strand, within RR42_RS02030 at 452.002 kb on - strand, within RR42_RS02030 at 452.267 kb on + strand at 452.267 kb on + strand at 452.296 kb on - strand at 452.296 kb on - strand at 452.298 kb on - strand at 452.339 kb on - strand at 452.339 kb on - strand at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.622 kb on - strand, within RR42_RS02035 at 452.622 kb on - strand, within RR42_RS02035 at 452.622 kb on - strand, within RR42_RS02035 at 452.622 kb on - strand, within RR42_RS02035 at 452.685 kb on - strand, within RR42_RS02035 at 452.867 kb on - strand, within RR42_RS02035 at 452.867 kb on - strand, within RR42_RS02035 at 452.867 kb on - strand, within RR42_RS02035 at 452.869 kb on - strand, within RR42_RS02035 at 453.018 kb on - strand, within RR42_RS02035 at 453.051 kb on - strand, within RR42_RS02035 at 453.105 kb on - strand, within RR42_RS02035 at 453.117 kb on - strand, within RR42_RS02035 at 453.135 kb on - strand, within RR42_RS02035 at 453.255 kb on - strand, within RR42_RS02035 at 453.891 kb on - strand, within RR42_RS02035 at 454.190 kb on - strand at 454.190 kb on - strand at 454.200 kb on + strand at 454.201 kb on - strand at 454.201 kb on - strand at 454.201 kb on - strand at 454.201 kb on - strand at 454.211 kb on + strand at 454.211 kb on + strand at 454.212 kb on - strand at 454.212 kb on - strand at 454.464 kb on + strand, within RR42_RS02040 at 454.464 kb on + strand, within RR42_RS02040 at 454.465 kb on - strand, within RR42_RS02040 at 454.465 kb on - strand, within RR42_RS02040 at 454.792 kb on - strand, within RR42_RS02040 at 454.792 kb on - strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 455.071 kb on - strand, within RR42_RS02040 at 455.148 kb on + strand at 455.148 kb on + strand at 455.148 kb on + strand at 455.148 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 451,820 - RR42_RS02030 0.47 -0.5 452,002 - RR42_RS02030 0.77 -0.3 452,002 - RR42_RS02030 0.77 +0.5 452,267 + +0.2 452,267 + +0.2 452,296 - -0.1 452,296 - -0.5 452,298 - +1.4 452,339 - -1.0 452,339 - -1.0 452,523 - RR42_RS02035 0.14 +0.7 452,523 - RR42_RS02035 0.14 +2.0 452,523 - RR42_RS02035 0.14 -1.3 452,523 - RR42_RS02035 0.14 -1.4 452,622 - RR42_RS02035 0.19 -1.3 452,622 - RR42_RS02035 0.19 -0.5 452,622 - RR42_RS02035 0.19 +0.2 452,622 - RR42_RS02035 0.19 -0.0 452,685 - RR42_RS02035 0.22 +0.4 452,867 - RR42_RS02035 0.32 +0.4 452,867 - RR42_RS02035 0.32 +0.4 452,867 - RR42_RS02035 0.32 -0.5 452,869 - RR42_RS02035 0.32 -0.1 453,018 - RR42_RS02035 0.40 -0.6 453,051 - RR42_RS02035 0.42 +1.6 453,105 - RR42_RS02035 0.45 +0.3 453,117 - RR42_RS02035 0.45 -0.9 453,135 - RR42_RS02035 0.46 +0.6 453,255 - RR42_RS02035 0.52 -0.3 453,891 - RR42_RS02035 0.86 +1.6 454,190 - -2.3 454,190 - +1.5 454,200 + +0.3 454,201 - +1.2 454,201 - +0.6 454,201 - -0.0 454,201 - -1.1 454,211 + -1.9 454,211 + +0.0 454,212 - +1.5 454,212 - +1.2 454,464 + RR42_RS02040 0.20 +1.0 454,464 + RR42_RS02040 0.20 +0.5 454,465 - RR42_RS02040 0.20 +0.4 454,465 - RR42_RS02040 0.20 -0.2 454,792 - RR42_RS02040 0.57 +2.5 454,792 - RR42_RS02040 0.57 +0.1 454,868 + RR42_RS02040 0.66 -0.2 454,868 + RR42_RS02040 0.66 +0.0 454,868 + RR42_RS02040 0.66 +0.2 454,868 + RR42_RS02040 0.66 -0.6 454,868 + RR42_RS02040 0.66 -0.0 454,869 - RR42_RS02040 0.66 -1.3 454,869 - RR42_RS02040 0.66 -1.4 454,869 - RR42_RS02040 0.66 +1.4 454,869 - RR42_RS02040 0.66 +1.5 454,869 - RR42_RS02040 0.66 +0.8 454,869 - RR42_RS02040 0.66 +0.3 454,869 - RR42_RS02040 0.66 +1.7 454,869 - RR42_RS02040 0.66 +0.6 454,872 + RR42_RS02040 0.66 -0.9 454,872 + RR42_RS02040 0.66 +0.8 454,872 + RR42_RS02040 0.66 -0.4 454,872 + RR42_RS02040 0.66 -0.1 454,872 + RR42_RS02040 0.66 -1.5 454,872 + RR42_RS02040 0.66 +0.4 454,872 + RR42_RS02040 0.66 -1.5 454,872 + RR42_RS02040 0.66 +1.3 454,872 + RR42_RS02040 0.66 -0.7 454,872 + RR42_RS02040 0.66 -0.5 454,872 + RR42_RS02040 0.66 -0.5 454,872 + RR42_RS02040 0.66 +0.9 454,873 - RR42_RS02040 0.66 -1.1 454,873 - RR42_RS02040 0.66 +1.0 454,873 - RR42_RS02040 0.66 -0.3 454,873 - RR42_RS02040 0.66 -0.9 454,873 - RR42_RS02040 0.66 +0.4 454,873 - RR42_RS02040 0.66 +0.6 454,873 - RR42_RS02040 0.66 +0.1 454,873 - RR42_RS02040 0.66 +0.2 454,873 - RR42_RS02040 0.66 +0.0 455,071 - RR42_RS02040 0.89 -0.9 455,148 + +0.7 455,148 + -1.2 455,148 + +1.0 455,148 + -0.1
Or see this region's nucleotide sequence