Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00895 and RR42_RS00900 are separated by 119 nucleotides RR42_RS00900 and RR42_RS00905 overlap by 4 nucleotides RR42_RS00905 and RR42_RS00910 overlap by 4 nucleotides
RR42_RS00895: RR42_RS00895 - acyl-CoA dehydrogenase, at 215,817 to 216,947
_RS00895
RR42_RS00900: RR42_RS00900 - 2-hydroxychromene-2-carboxylate isomerase, at 217,067 to 217,768
_RS00900
RR42_RS00905: RR42_RS00905 - haloacid dehalogenase, at 217,765 to 218,454
_RS00905
RR42_RS00910: RR42_RS00910 - hypothetical protein, at 218,451 to 218,876
_RS00910
Position (kb) 217
218 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 216.253 kb on - strand, within RR42_RS00895 at 216.479 kb on + strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.504 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.774 kb on + strand, within RR42_RS00895 at 216.774 kb on + strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.895 kb on - strand at 216.922 kb on + strand at 216.923 kb on - strand at 216.923 kb on - strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.089 kb on - strand at 217.154 kb on + strand, within RR42_RS00900 at 217.250 kb on + strand, within RR42_RS00900 at 217.292 kb on - strand, within RR42_RS00900 at 217.299 kb on - strand, within RR42_RS00900 at 217.730 kb on - strand at 217.899 kb on + strand, within RR42_RS00905 at 217.900 kb on - strand, within RR42_RS00905 at 218.202 kb on + strand, within RR42_RS00905 at 218.203 kb on - strand, within RR42_RS00905 at 218.203 kb on - strand, within RR42_RS00905 at 218.251 kb on + strand, within RR42_RS00905 at 218.251 kb on + strand, within RR42_RS00905 at 218.251 kb on + strand, within RR42_RS00905 at 218.252 kb on - strand, within RR42_RS00905 at 218.310 kb on + strand, within RR42_RS00905 at 218.425 kb on - strand at 218.592 kb on + strand, within RR42_RS00910 at 218.592 kb on + strand, within RR42_RS00910 at 218.592 kb on + strand, within RR42_RS00910 at 218.639 kb on + strand, within RR42_RS00910 at 218.639 kb on + strand, within RR42_RS00910 at 218.640 kb on - strand, within RR42_RS00910 at 218.640 kb on - strand, within RR42_RS00910 at 218.752 kb on - strand, within RR42_RS00910
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 216,253 - RR42_RS00895 0.39 -1.3 216,479 + RR42_RS00895 0.59 +1.6 216,480 - RR42_RS00895 0.59 +0.1 216,480 - RR42_RS00895 0.59 -1.6 216,480 - RR42_RS00895 0.59 +2.0 216,504 + RR42_RS00895 0.61 -0.2 216,600 + RR42_RS00895 0.69 +0.7 216,600 + RR42_RS00895 0.69 +0.0 216,600 + RR42_RS00895 0.69 +1.0 216,774 + RR42_RS00895 0.85 -0.5 216,774 + RR42_RS00895 0.85 +1.2 216,775 - RR42_RS00895 0.85 +0.7 216,775 - RR42_RS00895 0.85 +1.6 216,895 - -1.5 216,922 + -0.1 216,923 - +0.9 216,923 - +0.3 217,088 + -0.3 217,088 + -0.6 217,088 + -2.2 217,089 - -0.7 217,154 + RR42_RS00900 0.12 +0.3 217,250 + RR42_RS00900 0.26 -0.5 217,292 - RR42_RS00900 0.32 +3.6 217,299 - RR42_RS00900 0.33 +0.4 217,730 - -0.6 217,899 + RR42_RS00905 0.19 -0.8 217,900 - RR42_RS00905 0.20 +0.5 218,202 + RR42_RS00905 0.63 +0.0 218,203 - RR42_RS00905 0.63 -0.6 218,203 - RR42_RS00905 0.63 -0.3 218,251 + RR42_RS00905 0.70 -0.4 218,251 + RR42_RS00905 0.70 -1.2 218,251 + RR42_RS00905 0.70 +0.3 218,252 - RR42_RS00905 0.71 +0.3 218,310 + RR42_RS00905 0.79 +0.6 218,425 - -1.2 218,592 + RR42_RS00910 0.33 -0.4 218,592 + RR42_RS00910 0.33 +1.0 218,592 + RR42_RS00910 0.33 -0.4 218,639 + RR42_RS00910 0.44 +1.0 218,639 + RR42_RS00910 0.44 +0.0 218,640 - RR42_RS00910 0.44 +0.9 218,640 - RR42_RS00910 0.44 -0.4 218,752 - RR42_RS00910 0.71 +0.2
Or see this region's nucleotide sequence