Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00230 and RR42_RS00235 are separated by 135 nucleotides RR42_RS00235 and RR42_RS00240 are separated by 93 nucleotides RR42_RS00240 and RR42_RS00245 are separated by 253 nucleotides
RR42_RS00230: RR42_RS00230 - ADP-ribosylglycohydrolase, at 71,437 to 72,531
_RS00230
RR42_RS00235: RR42_RS00235 - membrane protein, at 72,667 to 73,938
_RS00235
RR42_RS00240: RR42_RS00240 - nucleotide pyrophosphohydrolase, at 74,032 to 74,367
_RS00240
RR42_RS00245: RR42_RS00245 - microcystin degradation protein MlrC, at 74,621 to 76,102
_RS00245
Position (kb)
72
73
74 Strain fitness (log2 ratio)
-1
0
1
2
3 at 71.704 kb on + strand, within RR42_RS00230 at 71.705 kb on - strand, within RR42_RS00230 at 71.782 kb on + strand, within RR42_RS00230 at 72.517 kb on + strand at 72.561 kb on + strand at 72.573 kb on + strand at 73.108 kb on - strand, within RR42_RS00235 at 73.500 kb on - strand, within RR42_RS00235 at 73.558 kb on + strand, within RR42_RS00235 at 73.559 kb on - strand, within RR42_RS00235 at 73.771 kb on - strand, within RR42_RS00235 at 73.850 kb on - strand at 73.944 kb on - strand at 74.010 kb on + strand at 74.104 kb on + strand, within RR42_RS00240 at 74.105 kb on - strand, within RR42_RS00240 at 74.105 kb on - strand, within RR42_RS00240 at 74.105 kb on - strand, within RR42_RS00240 at 74.105 kb on - strand, within RR42_RS00240 at 74.257 kb on + strand, within RR42_RS00240 at 74.258 kb on - strand, within RR42_RS00240 at 74.659 kb on + strand at 74.660 kb on - strand at 74.660 kb on - strand at 74.850 kb on - strand, within RR42_RS00245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 71,704 + RR42_RS00230 0.24 +1.5 71,705 - RR42_RS00230 0.24 -0.5 71,782 + RR42_RS00230 0.32 +0.2 72,517 + +0.6 72,561 + +0.1 72,573 + -0.1 73,108 - RR42_RS00235 0.35 -0.8 73,500 - RR42_RS00235 0.65 -1.2 73,558 + RR42_RS00235 0.70 -1.6 73,559 - RR42_RS00235 0.70 +0.6 73,771 - RR42_RS00235 0.87 -0.1 73,850 - +0.3 73,944 - -0.8 74,010 + -0.7 74,104 + RR42_RS00240 0.21 -0.9 74,105 - RR42_RS00240 0.22 +1.1 74,105 - RR42_RS00240 0.22 +1.6 74,105 - RR42_RS00240 0.22 +0.2 74,105 - RR42_RS00240 0.22 +0.1 74,257 + RR42_RS00240 0.67 -0.6 74,258 - RR42_RS00240 0.67 -0.8 74,659 + -0.2 74,660 - +3.6 74,660 - -1.2 74,850 - RR42_RS00245 0.15 +0.2
Or see this region's nucleotide sequence