Experiment: D-Xylose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5234 and Pf6N2E2_5235 are separated by 2 nucleotides Pf6N2E2_5235 and Pf6N2E2_5236 are separated by 62 nucleotides Pf6N2E2_5236 and Pf6N2E2_5237 overlap by 21 nucleotides Pf6N2E2_5237 and Pf6N2E2_5238 are separated by 3 nucleotides
Pf6N2E2_5234 - Uracil permease, at 6,032,089 to 6,033,363
_5234
Pf6N2E2_5235 - Uracil phosphoribosyltransferase (EC 2.4.2.9), at 6,033,366 to 6,034,004
_5235
Pf6N2E2_5236 - hypothetical protein, at 6,034,067 to 6,034,189
_5236
Pf6N2E2_5237 - Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8), at 6,034,169 to 6,034,726
_5237
Pf6N2E2_5238 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 6,034,730 to 6,035,203
_5238
Position (kb)
6034
6035 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6033.074 kb on + strand, within Pf6N2E2_5234 at 6033.075 kb on - strand, within Pf6N2E2_5234 at 6033.075 kb on - strand, within Pf6N2E2_5234 at 6033.249 kb on + strand at 6033.250 kb on - strand at 6033.280 kb on + strand at 6033.280 kb on + strand at 6033.430 kb on + strand, within Pf6N2E2_5235 at 6033.430 kb on + strand, within Pf6N2E2_5235 at 6033.430 kb on + strand, within Pf6N2E2_5235 at 6033.469 kb on + strand, within Pf6N2E2_5235 at 6033.469 kb on + strand, within Pf6N2E2_5235 at 6033.469 kb on + strand, within Pf6N2E2_5235 at 6033.469 kb on + strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.667 kb on + strand, within Pf6N2E2_5235 at 6033.667 kb on + strand, within Pf6N2E2_5235 at 6033.668 kb on - strand, within Pf6N2E2_5235 at 6033.668 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.883 kb on + strand, within Pf6N2E2_5235 at 6033.883 kb on + strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6034.041 kb on - strand at 6034.045 kb on + strand at 6034.046 kb on - strand at 6034.050 kb on + strand at 6034.050 kb on + strand at 6034.050 kb on + strand at 6034.051 kb on - strand at 6034.051 kb on - strand at 6034.071 kb on - strand at 6034.077 kb on + strand at 6034.077 kb on + strand at 6034.078 kb on - strand at 6034.088 kb on - strand, within Pf6N2E2_5236 at 6034.088 kb on - strand, within Pf6N2E2_5236 at 6034.125 kb on - strand, within Pf6N2E2_5236 at 6034.125 kb on - strand, within Pf6N2E2_5236 at 6034.224 kb on - strand at 6034.383 kb on - strand, within Pf6N2E2_5237 at 6034.650 kb on - strand, within Pf6N2E2_5237 at 6034.650 kb on - strand, within Pf6N2E2_5237 at 6034.673 kb on + strand at 6034.673 kb on + strand at 6034.673 kb on + strand at 6034.673 kb on + strand at 6034.674 kb on - strand at 6034.674 kb on - strand at 6034.674 kb on - strand at 6034.724 kb on + strand at 6034.724 kb on + strand at 6034.724 kb on + strand at 6034.724 kb on + strand at 6034.725 kb on - strand at 6034.725 kb on - strand at 6034.816 kb on + strand, within Pf6N2E2_5238 at 6034.816 kb on + strand, within Pf6N2E2_5238 at 6034.817 kb on - strand, within Pf6N2E2_5238 at 6034.817 kb on - strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6035.042 kb on + strand, within Pf6N2E2_5238
Per-strain Table
Position Strand Gene LocusTag Fraction D-Xylose (C) remove 6,033,074 + Pf6N2E2_5234 0.77 +0.9 6,033,075 - Pf6N2E2_5234 0.77 -1.1 6,033,075 - Pf6N2E2_5234 0.77 -0.0 6,033,249 + +0.2 6,033,250 - +0.1 6,033,280 + +0.3 6,033,280 + +0.7 6,033,430 + Pf6N2E2_5235 0.10 +0.2 6,033,430 + Pf6N2E2_5235 0.10 +0.1 6,033,430 + Pf6N2E2_5235 0.10 +0.2 6,033,469 + Pf6N2E2_5235 0.16 +0.0 6,033,469 + Pf6N2E2_5235 0.16 -1.6 6,033,469 + Pf6N2E2_5235 0.16 +0.6 6,033,469 + Pf6N2E2_5235 0.16 +0.1 6,033,470 - Pf6N2E2_5235 0.16 +0.6 6,033,470 - Pf6N2E2_5235 0.16 +1.2 6,033,470 - Pf6N2E2_5235 0.16 +0.1 6,033,470 - Pf6N2E2_5235 0.16 -1.5 6,033,470 - Pf6N2E2_5235 0.16 -1.3 6,033,470 - Pf6N2E2_5235 0.16 +0.6 6,033,470 - Pf6N2E2_5235 0.16 +0.7 6,033,470 - Pf6N2E2_5235 0.16 +0.6 6,033,667 + Pf6N2E2_5235 0.47 -0.4 6,033,667 + Pf6N2E2_5235 0.47 -0.6 6,033,668 - Pf6N2E2_5235 0.47 -0.2 6,033,668 - Pf6N2E2_5235 0.47 +0.1 6,033,851 - Pf6N2E2_5235 0.76 +0.2 6,033,851 - Pf6N2E2_5235 0.76 -1.0 6,033,851 - Pf6N2E2_5235 0.76 +0.4 6,033,851 - Pf6N2E2_5235 0.76 +1.1 6,033,851 - Pf6N2E2_5235 0.76 -0.0 6,033,883 + Pf6N2E2_5235 0.81 -0.8 6,033,883 + Pf6N2E2_5235 0.81 -1.8 6,033,884 - Pf6N2E2_5235 0.81 +0.7 6,033,884 - Pf6N2E2_5235 0.81 +0.9 6,033,884 - Pf6N2E2_5235 0.81 -0.7 6,033,884 - Pf6N2E2_5235 0.81 +0.3 6,033,884 - Pf6N2E2_5235 0.81 +0.1 6,034,041 - +0.5 6,034,045 + -1.2 6,034,046 - -0.2 6,034,050 + -0.5 6,034,050 + -0.8 6,034,050 + +0.5 6,034,051 - -1.0 6,034,051 - -0.2 6,034,071 - +0.4 6,034,077 + -0.7 6,034,077 + -0.4 6,034,078 - -0.9 6,034,088 - Pf6N2E2_5236 0.17 -0.7 6,034,088 - Pf6N2E2_5236 0.17 -0.6 6,034,125 - Pf6N2E2_5236 0.47 -1.5 6,034,125 - Pf6N2E2_5236 0.47 -0.5 6,034,224 - +0.3 6,034,383 - Pf6N2E2_5237 0.38 +0.3 6,034,650 - Pf6N2E2_5237 0.86 +0.4 6,034,650 - Pf6N2E2_5237 0.86 +1.5 6,034,673 + +0.6 6,034,673 + +0.4 6,034,673 + -1.4 6,034,673 + -0.8 6,034,674 - +0.5 6,034,674 - +0.1 6,034,674 - -0.8 6,034,724 + +0.9 6,034,724 + -0.6 6,034,724 + +1.6 6,034,724 + +0.4 6,034,725 - +0.1 6,034,725 - +0.2 6,034,816 + Pf6N2E2_5238 0.18 -0.3 6,034,816 + Pf6N2E2_5238 0.18 +0.2 6,034,817 - Pf6N2E2_5238 0.18 +0.4 6,034,817 - Pf6N2E2_5238 0.18 -1.3 6,034,877 + Pf6N2E2_5238 0.31 -1.0 6,034,877 + Pf6N2E2_5238 0.31 +0.8 6,034,877 + Pf6N2E2_5238 0.31 -0.7 6,034,877 + Pf6N2E2_5238 0.31 +0.0 6,034,878 - Pf6N2E2_5238 0.31 +1.8 6,034,878 - Pf6N2E2_5238 0.31 +0.8 6,034,878 - Pf6N2E2_5238 0.31 -0.2 6,034,878 - Pf6N2E2_5238 0.31 -0.2 6,035,042 + Pf6N2E2_5238 0.66 +1.0
Or see this region's nucleotide sequence