Experiment: D-Glucose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4593 and Pf1N1B4_4594 overlap by 4 nucleotides Pf1N1B4_4594 and Pf1N1B4_4595 are separated by 282 nucleotides
Pf1N1B4_4593 - ABC transporter, periplasmic substrate-binding protein, at 5,064,001 to 5,065,842
_4593
Pf1N1B4_4594 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 5,065,839 to 5,067,671
_4594
Pf1N1B4_4595 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,067,954 to 5,069,408
_4595
Position (kb)
5065
5066
5067
5068 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5064.983 kb on + strand, within Pf1N1B4_4593 at 5064.984 kb on - strand, within Pf1N1B4_4593 at 5064.984 kb on - strand, within Pf1N1B4_4593 at 5064.984 kb on - strand, within Pf1N1B4_4593 at 5065.448 kb on - strand, within Pf1N1B4_4593 at 5065.487 kb on + strand, within Pf1N1B4_4593 at 5065.488 kb on - strand, within Pf1N1B4_4593 at 5065.589 kb on + strand, within Pf1N1B4_4593 at 5065.703 kb on - strand at 5065.942 kb on + strand at 5065.942 kb on + strand at 5065.942 kb on + strand at 5065.969 kb on + strand at 5065.970 kb on - strand at 5065.970 kb on - strand at 5066.218 kb on + strand, within Pf1N1B4_4594 at 5066.570 kb on - strand at 5066.588 kb on - strand, within Pf1N1B4_4594 at 5066.633 kb on + strand, within Pf1N1B4_4594 at 5066.894 kb on - strand, within Pf1N1B4_4594 at 5067.212 kb on - strand, within Pf1N1B4_4594 at 5067.223 kb on - strand, within Pf1N1B4_4594 at 5067.763 kb on + strand at 5068.067 kb on - strand at 5068.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) remove 5,064,983 + Pf1N1B4_4593 0.53 +1.6 5,064,984 - Pf1N1B4_4593 0.53 -1.3 5,064,984 - Pf1N1B4_4593 0.53 -1.0 5,064,984 - Pf1N1B4_4593 0.53 -1.5 5,065,448 - Pf1N1B4_4593 0.79 -0.6 5,065,487 + Pf1N1B4_4593 0.81 +0.0 5,065,488 - Pf1N1B4_4593 0.81 -1.0 5,065,589 + Pf1N1B4_4593 0.86 -0.6 5,065,703 - -0.1 5,065,942 + +0.0 5,065,942 + -1.2 5,065,942 + +2.0 5,065,969 + -1.0 5,065,970 - -1.0 5,065,970 - +1.0 5,066,218 + Pf1N1B4_4594 0.21 -2.6 5,066,570 - +0.7 5,066,588 - Pf1N1B4_4594 0.41 -1.0 5,066,633 + Pf1N1B4_4594 0.43 -2.6 5,066,894 - Pf1N1B4_4594 0.58 +0.7 5,067,212 - Pf1N1B4_4594 0.75 -0.6 5,067,223 - Pf1N1B4_4594 0.76 +0.4 5,067,763 + +0.6 5,068,067 - -0.6 5,068,067 - -2.0
Or see this region's nucleotide sequence