Experiment: R2A with copper (II) chloride 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09205 and CA265_RS09210 overlap by 4 nucleotides CA265_RS09210 and CA265_RS09215 are separated by 9 nucleotides
CA265_RS09205: CA265_RS09205 - ligand-gated channel protein, at 2,294,836 to 2,296,872
_RS09205
CA265_RS09210: CA265_RS09210 - hypothetical protein, at 2,296,869 to 2,298,002
_RS09210
CA265_RS09215: CA265_RS09215 - hypothetical protein, at 2,298,012 to 2,299,127
_RS09215
Position (kb)
2296
2297
2298
2299 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2295.874 kb on + strand, within CA265_RS09205 at 2295.875 kb on - strand, within CA265_RS09205 at 2295.925 kb on + strand, within CA265_RS09205 at 2295.953 kb on + strand, within CA265_RS09205 at 2295.966 kb on - strand, within CA265_RS09205 at 2295.966 kb on - strand, within CA265_RS09205 at 2295.966 kb on - strand, within CA265_RS09205 at 2295.968 kb on - strand, within CA265_RS09205 at 2295.994 kb on - strand, within CA265_RS09205 at 2296.038 kb on + strand, within CA265_RS09205 at 2296.039 kb on - strand, within CA265_RS09205 at 2296.039 kb on - strand, within CA265_RS09205 at 2296.107 kb on + strand, within CA265_RS09205 at 2296.131 kb on - strand, within CA265_RS09205 at 2296.148 kb on + strand, within CA265_RS09205 at 2296.149 kb on - strand, within CA265_RS09205 at 2296.152 kb on - strand, within CA265_RS09205 at 2296.183 kb on + strand, within CA265_RS09205 at 2296.183 kb on + strand, within CA265_RS09205 at 2296.184 kb on - strand, within CA265_RS09205 at 2296.223 kb on - strand, within CA265_RS09205 at 2296.228 kb on - strand, within CA265_RS09205 at 2296.228 kb on - strand, within CA265_RS09205 at 2296.273 kb on + strand, within CA265_RS09205 at 2296.274 kb on - strand, within CA265_RS09205 at 2296.348 kb on + strand, within CA265_RS09205 at 2296.348 kb on + strand, within CA265_RS09205 at 2296.352 kb on - strand, within CA265_RS09205 at 2296.352 kb on - strand, within CA265_RS09205 at 2296.353 kb on + strand, within CA265_RS09205 at 2296.400 kb on - strand, within CA265_RS09205 at 2296.415 kb on - strand, within CA265_RS09205 at 2296.415 kb on - strand, within CA265_RS09205 at 2296.513 kb on - strand, within CA265_RS09205 at 2296.576 kb on + strand, within CA265_RS09205 at 2296.577 kb on - strand, within CA265_RS09205 at 2296.591 kb on - strand, within CA265_RS09205 at 2296.619 kb on - strand, within CA265_RS09205 at 2296.652 kb on - strand, within CA265_RS09205 at 2296.686 kb on + strand at 2296.696 kb on + strand at 2296.697 kb on - strand at 2296.697 kb on - strand at 2296.711 kb on + strand at 2296.712 kb on - strand at 2296.736 kb on - strand at 2296.763 kb on + strand at 2296.768 kb on - strand at 2296.843 kb on + strand at 2296.963 kb on + strand at 2297.027 kb on + strand, within CA265_RS09210 at 2297.040 kb on - strand, within CA265_RS09210 at 2297.057 kb on + strand, within CA265_RS09210 at 2297.079 kb on + strand, within CA265_RS09210 at 2297.079 kb on + strand, within CA265_RS09210 at 2297.104 kb on + strand, within CA265_RS09210 at 2297.104 kb on + strand, within CA265_RS09210 at 2297.148 kb on - strand, within CA265_RS09210 at 2297.153 kb on + strand, within CA265_RS09210 at 2297.154 kb on - strand, within CA265_RS09210 at 2297.188 kb on + strand, within CA265_RS09210 at 2297.246 kb on + strand, within CA265_RS09210 at 2297.262 kb on + strand, within CA265_RS09210 at 2297.293 kb on + strand, within CA265_RS09210 at 2297.293 kb on + strand, within CA265_RS09210 at 2297.294 kb on - strand, within CA265_RS09210 at 2297.301 kb on + strand, within CA265_RS09210 at 2297.334 kb on - strand, within CA265_RS09210 at 2297.480 kb on + strand, within CA265_RS09210 at 2297.481 kb on - strand, within CA265_RS09210 at 2297.569 kb on - strand, within CA265_RS09210 at 2297.569 kb on - strand, within CA265_RS09210 at 2297.605 kb on - strand, within CA265_RS09210 at 2297.704 kb on - strand, within CA265_RS09210 at 2297.710 kb on - strand, within CA265_RS09210 at 2297.712 kb on - strand, within CA265_RS09210 at 2297.716 kb on - strand, within CA265_RS09210 at 2297.761 kb on - strand, within CA265_RS09210 at 2297.880 kb on + strand, within CA265_RS09210 at 2297.881 kb on - strand, within CA265_RS09210 at 2297.906 kb on + strand at 2297.928 kb on + strand at 2297.928 kb on + strand at 2297.928 kb on + strand at 2297.929 kb on - strand at 2298.008 kb on + strand at 2298.009 kb on - strand at 2298.068 kb on + strand at 2298.068 kb on + strand at 2298.068 kb on + strand at 2298.068 kb on + strand at 2298.068 kb on + strand at 2298.081 kb on - strand at 2298.141 kb on + strand, within CA265_RS09215 at 2298.142 kb on - strand, within CA265_RS09215 at 2298.189 kb on + strand, within CA265_RS09215 at 2298.192 kb on + strand, within CA265_RS09215 at 2298.197 kb on + strand, within CA265_RS09215 at 2298.219 kb on - strand, within CA265_RS09215 at 2298.238 kb on + strand, within CA265_RS09215 at 2298.282 kb on + strand, within CA265_RS09215 at 2298.360 kb on - strand, within CA265_RS09215 at 2298.447 kb on + strand, within CA265_RS09215 at 2298.448 kb on - strand, within CA265_RS09215 at 2298.451 kb on + strand, within CA265_RS09215 at 2298.459 kb on + strand, within CA265_RS09215 at 2298.467 kb on + strand, within CA265_RS09215 at 2298.514 kb on + strand, within CA265_RS09215 at 2298.564 kb on + strand, within CA265_RS09215 at 2298.565 kb on - strand, within CA265_RS09215 at 2298.611 kb on + strand, within CA265_RS09215 at 2298.611 kb on + strand, within CA265_RS09215 at 2298.710 kb on - strand, within CA265_RS09215 at 2298.719 kb on - strand, within CA265_RS09215 at 2298.732 kb on + strand, within CA265_RS09215 at 2298.733 kb on - strand, within CA265_RS09215 at 2298.733 kb on - strand, within CA265_RS09215 at 2298.733 kb on - strand, within CA265_RS09215 at 2298.734 kb on + strand, within CA265_RS09215 at 2298.799 kb on - strand, within CA265_RS09215 at 2298.799 kb on - strand, within CA265_RS09215 at 2298.843 kb on + strand, within CA265_RS09215 at 2298.844 kb on - strand, within CA265_RS09215 at 2298.844 kb on - strand, within CA265_RS09215 at 2298.844 kb on - strand, within CA265_RS09215 at 2298.847 kb on + strand, within CA265_RS09215 at 2298.857 kb on + strand, within CA265_RS09215 at 2298.858 kb on - strand, within CA265_RS09215 at 2298.862 kb on - strand, within CA265_RS09215 at 2298.891 kb on - strand, within CA265_RS09215 at 2298.919 kb on + strand, within CA265_RS09215 at 2298.945 kb on - strand, within CA265_RS09215 at 2298.945 kb on - strand, within CA265_RS09215
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.16 mM remove 2,295,874 + CA265_RS09205 0.51 +0.4 2,295,875 - CA265_RS09205 0.51 +0.9 2,295,925 + CA265_RS09205 0.53 +0.6 2,295,953 + CA265_RS09205 0.55 -0.1 2,295,966 - CA265_RS09205 0.55 -0.5 2,295,966 - CA265_RS09205 0.55 -0.3 2,295,966 - CA265_RS09205 0.55 -2.3 2,295,968 - CA265_RS09205 0.56 -2.7 2,295,994 - CA265_RS09205 0.57 -1.3 2,296,038 + CA265_RS09205 0.59 +0.2 2,296,039 - CA265_RS09205 0.59 +0.1 2,296,039 - CA265_RS09205 0.59 -0.9 2,296,107 + CA265_RS09205 0.62 +0.3 2,296,131 - CA265_RS09205 0.64 -1.7 2,296,148 + CA265_RS09205 0.64 -0.7 2,296,149 - CA265_RS09205 0.64 -1.8 2,296,152 - CA265_RS09205 0.65 -0.3 2,296,183 + CA265_RS09205 0.66 +0.7 2,296,183 + CA265_RS09205 0.66 +0.2 2,296,184 - CA265_RS09205 0.66 -1.0 2,296,223 - CA265_RS09205 0.68 +0.1 2,296,228 - CA265_RS09205 0.68 -0.3 2,296,228 - CA265_RS09205 0.68 -0.4 2,296,273 + CA265_RS09205 0.71 +0.4 2,296,274 - CA265_RS09205 0.71 -0.8 2,296,348 + CA265_RS09205 0.74 -1.5 2,296,348 + CA265_RS09205 0.74 -1.2 2,296,352 - CA265_RS09205 0.74 -1.2 2,296,352 - CA265_RS09205 0.74 +0.8 2,296,353 + CA265_RS09205 0.74 -0.8 2,296,400 - CA265_RS09205 0.77 -0.3 2,296,415 - CA265_RS09205 0.78 -0.1 2,296,415 - CA265_RS09205 0.78 +0.5 2,296,513 - CA265_RS09205 0.82 -0.3 2,296,576 + CA265_RS09205 0.85 -1.6 2,296,577 - CA265_RS09205 0.85 -1.5 2,296,591 - CA265_RS09205 0.86 -0.9 2,296,619 - CA265_RS09205 0.88 -1.3 2,296,652 - CA265_RS09205 0.89 +0.2 2,296,686 + +0.1 2,296,696 + +1.1 2,296,697 - +0.7 2,296,697 - +0.6 2,296,711 + +0.4 2,296,712 - -1.2 2,296,736 - +1.3 2,296,763 + -1.1 2,296,768 - -3.6 2,296,843 + -0.0 2,296,963 + +0.7 2,297,027 + CA265_RS09210 0.14 +0.2 2,297,040 - CA265_RS09210 0.15 -1.4 2,297,057 + CA265_RS09210 0.17 +0.8 2,297,079 + CA265_RS09210 0.19 +1.1 2,297,079 + CA265_RS09210 0.19 +0.1 2,297,104 + CA265_RS09210 0.21 +0.4 2,297,104 + CA265_RS09210 0.21 +0.1 2,297,148 - CA265_RS09210 0.25 -1.0 2,297,153 + CA265_RS09210 0.25 -0.9 2,297,154 - CA265_RS09210 0.25 +0.5 2,297,188 + CA265_RS09210 0.28 -2.2 2,297,246 + CA265_RS09210 0.33 -0.2 2,297,262 + CA265_RS09210 0.35 +0.7 2,297,293 + CA265_RS09210 0.37 -3.6 2,297,293 + CA265_RS09210 0.37 -0.6 2,297,294 - CA265_RS09210 0.37 -0.8 2,297,301 + CA265_RS09210 0.38 -1.5 2,297,334 - CA265_RS09210 0.41 -0.9 2,297,480 + CA265_RS09210 0.54 +0.0 2,297,481 - CA265_RS09210 0.54 -1.8 2,297,569 - CA265_RS09210 0.62 -1.2 2,297,569 - CA265_RS09210 0.62 +0.3 2,297,605 - CA265_RS09210 0.65 -2.1 2,297,704 - CA265_RS09210 0.74 +0.1 2,297,710 - CA265_RS09210 0.74 -0.7 2,297,712 - CA265_RS09210 0.74 -1.3 2,297,716 - CA265_RS09210 0.75 -0.7 2,297,761 - CA265_RS09210 0.79 +0.4 2,297,880 + CA265_RS09210 0.89 +1.2 2,297,881 - CA265_RS09210 0.89 -3.8 2,297,906 + -0.4 2,297,928 + +0.5 2,297,928 + +0.2 2,297,928 + +1.3 2,297,929 - -1.9 2,298,008 + +0.3 2,298,009 - +0.3 2,298,068 + +0.9 2,298,068 + +0.4 2,298,068 + +0.1 2,298,068 + +0.4 2,298,068 + +0.6 2,298,081 - -0.1 2,298,141 + CA265_RS09215 0.12 -0.6 2,298,142 - CA265_RS09215 0.12 -1.2 2,298,189 + CA265_RS09215 0.16 +0.4 2,298,192 + CA265_RS09215 0.16 +0.4 2,298,197 + CA265_RS09215 0.17 -1.8 2,298,219 - CA265_RS09215 0.19 +0.2 2,298,238 + CA265_RS09215 0.20 +0.9 2,298,282 + CA265_RS09215 0.24 -0.2 2,298,360 - CA265_RS09215 0.31 -0.5 2,298,447 + CA265_RS09215 0.39 +0.3 2,298,448 - CA265_RS09215 0.39 +0.3 2,298,451 + CA265_RS09215 0.39 +0.7 2,298,459 + CA265_RS09215 0.40 -1.0 2,298,467 + CA265_RS09215 0.41 +0.1 2,298,514 + CA265_RS09215 0.45 -0.3 2,298,564 + CA265_RS09215 0.49 +1.2 2,298,565 - CA265_RS09215 0.50 +1.5 2,298,611 + CA265_RS09215 0.54 +0.5 2,298,611 + CA265_RS09215 0.54 +1.7 2,298,710 - CA265_RS09215 0.63 -0.5 2,298,719 - CA265_RS09215 0.63 -1.8 2,298,732 + CA265_RS09215 0.65 -0.1 2,298,733 - CA265_RS09215 0.65 -0.7 2,298,733 - CA265_RS09215 0.65 -0.4 2,298,733 - CA265_RS09215 0.65 -2.3 2,298,734 + CA265_RS09215 0.65 -1.2 2,298,799 - CA265_RS09215 0.71 -0.2 2,298,799 - CA265_RS09215 0.71 -0.3 2,298,843 + CA265_RS09215 0.74 -1.4 2,298,844 - CA265_RS09215 0.75 -0.8 2,298,844 - CA265_RS09215 0.75 +1.7 2,298,844 - CA265_RS09215 0.75 -1.8 2,298,847 + CA265_RS09215 0.75 +0.3 2,298,857 + CA265_RS09215 0.76 +0.3 2,298,858 - CA265_RS09215 0.76 +0.4 2,298,862 - CA265_RS09215 0.76 -0.6 2,298,891 - CA265_RS09215 0.79 -0.3 2,298,919 + CA265_RS09215 0.81 -0.1 2,298,945 - CA265_RS09215 0.84 -0.9 2,298,945 - CA265_RS09215 0.84 -1.7
Or see this region's nucleotide sequence