Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS34200

Experiment: R2A with copper (II) chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS34195 and RR42_RS34200 are separated by 476 nucleotidesRR42_RS34200 and RR42_RS34205 are separated by 324 nucleotidesRR42_RS34205 and RR42_RS34210 are separated by 220 nucleotides RR42_RS34195: RR42_RS34195 - amino acid transporter LysE, at 3,097,341 to 3,097,952 _RS34195 RR42_RS34200: RR42_RS34200 - membrane protein, at 3,098,429 to 3,099,205 _RS34200 RR42_RS34205: RR42_RS34205 - hypothetical protein, at 3,099,530 to 3,099,880 _RS34205 RR42_RS34210: RR42_RS34210 - hypothetical protein, at 3,100,101 to 3,100,490 _RS34210 Position (kb) 3098 3099 3100Strain fitness (log2 ratio) -2 -1 0 1 2at 3097.584 kb on + strand, within RR42_RS34195at 3097.603 kb on + strand, within RR42_RS34195at 3097.603 kb on + strand, within RR42_RS34195at 3097.655 kb on - strand, within RR42_RS34195at 3097.803 kb on + strand, within RR42_RS34195at 3097.803 kb on + strand, within RR42_RS34195at 3097.804 kb on - strand, within RR42_RS34195at 3097.804 kb on - strand, within RR42_RS34195at 3097.804 kb on - strand, within RR42_RS34195at 3097.858 kb on + strand, within RR42_RS34195at 3097.858 kb on + strand, within RR42_RS34195at 3097.859 kb on - strand, within RR42_RS34195at 3097.950 kb on + strandat 3097.950 kb on + strandat 3097.951 kb on - strandat 3098.109 kb on + strandat 3098.110 kb on - strandat 3098.174 kb on + strandat 3098.175 kb on - strandat 3098.187 kb on + strandat 3098.187 kb on + strandat 3098.188 kb on - strandat 3098.188 kb on - strandat 3098.188 kb on - strandat 3098.314 kb on + strandat 3098.315 kb on - strandat 3098.657 kb on + strand, within RR42_RS34200at 3098.657 kb on + strand, within RR42_RS34200at 3098.657 kb on + strand, within RR42_RS34200at 3098.658 kb on - strand, within RR42_RS34200at 3098.804 kb on + strand, within RR42_RS34200at 3098.804 kb on + strand, within RR42_RS34200at 3098.804 kb on + strand, within RR42_RS34200at 3098.804 kb on + strand, within RR42_RS34200at 3098.805 kb on - strand, within RR42_RS34200at 3098.884 kb on + strand, within RR42_RS34200at 3098.885 kb on - strand, within RR42_RS34200at 3098.885 kb on - strand, within RR42_RS34200at 3098.921 kb on + strand, within RR42_RS34200at 3098.921 kb on + strand, within RR42_RS34200at 3098.922 kb on - strand, within RR42_RS34200at 3099.074 kb on + strand, within RR42_RS34200at 3099.074 kb on + strand, within RR42_RS34200at 3099.120 kb on - strand, within RR42_RS34200at 3099.327 kb on - strandat 3099.434 kb on - strandat 3099.434 kb on - strandat 3099.476 kb on - strandat 3099.535 kb on + strandat 3099.588 kb on - strand, within RR42_RS34205at 3099.792 kb on - strand, within RR42_RS34205at 3099.806 kb on + strand, within RR42_RS34205at 3099.807 kb on - strand, within RR42_RS34205at 3099.807 kb on - strand, within RR42_RS34205at 3099.939 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM
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3,097,584 + RR42_RS34195 0.40 +0.5
3,097,603 + RR42_RS34195 0.43 -1.4
3,097,603 + RR42_RS34195 0.43 +0.6
3,097,655 - RR42_RS34195 0.51 -2.0
3,097,803 + RR42_RS34195 0.75 -1.2
3,097,803 + RR42_RS34195 0.75 -0.2
3,097,804 - RR42_RS34195 0.76 +2.7
3,097,804 - RR42_RS34195 0.76 -0.1
3,097,804 - RR42_RS34195 0.76 -0.2
3,097,858 + RR42_RS34195 0.84 -0.2
3,097,858 + RR42_RS34195 0.84 +1.1
3,097,859 - RR42_RS34195 0.85 -1.4
3,097,950 + -0.4
3,097,950 + -0.8
3,097,951 - -0.1
3,098,109 + +0.8
3,098,110 - -0.1
3,098,174 + -1.0
3,098,175 - +0.7
3,098,187 + -1.0
3,098,187 + -0.3
3,098,188 - -0.1
3,098,188 - +1.8
3,098,188 - +0.6
3,098,314 + -0.4
3,098,315 - -0.6
3,098,657 + RR42_RS34200 0.29 +0.5
3,098,657 + RR42_RS34200 0.29 -0.0
3,098,657 + RR42_RS34200 0.29 -0.7
3,098,658 - RR42_RS34200 0.29 +0.6
3,098,804 + RR42_RS34200 0.48 +0.9
3,098,804 + RR42_RS34200 0.48 +0.2
3,098,804 + RR42_RS34200 0.48 -0.0
3,098,804 + RR42_RS34200 0.48 -1.2
3,098,805 - RR42_RS34200 0.48 +0.0
3,098,884 + RR42_RS34200 0.59 +0.2
3,098,885 - RR42_RS34200 0.59 -0.5
3,098,885 - RR42_RS34200 0.59 -0.6
3,098,921 + RR42_RS34200 0.63 -0.1
3,098,921 + RR42_RS34200 0.63 +0.4
3,098,922 - RR42_RS34200 0.63 -0.4
3,099,074 + RR42_RS34200 0.83 +0.5
3,099,074 + RR42_RS34200 0.83 +0.4
3,099,120 - RR42_RS34200 0.89 +0.9
3,099,327 - +0.0
3,099,434 - -0.7
3,099,434 - +0.6
3,099,476 - +0.7
3,099,535 + -0.1
3,099,588 - RR42_RS34205 0.17 -0.7
3,099,792 - RR42_RS34205 0.75 -0.6
3,099,806 + RR42_RS34205 0.79 +0.1
3,099,807 - RR42_RS34205 0.79 -1.2
3,099,807 - RR42_RS34205 0.79 +0.1
3,099,939 + -0.0

Or see this region's nucleotide sequence