Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28295 and RR42_RS28300 overlap by 4 nucleotides RR42_RS28300 and RR42_RS28305 are separated by 474 nucleotides
RR42_RS28295: RR42_RS28295 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data) , at 1,708,250 to 1,709,452
_RS28295
RR42_RS28300: RR42_RS28300 - L-threonine 3-dehydrogenase (EC 1.1.1.103) (from data) , at 1,709,449 to 1,710,423
_RS28300
RR42_RS28305: RR42_RS28305 - L-threonine:H+ symporter (from data) , at 1,710,898 to 1,712,316
_RS28305
Position (kb)
1709
1710
1711 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1708.498 kb on + strand, within RR42_RS28295 at 1708.499 kb on - strand, within RR42_RS28295 at 1708.499 kb on - strand, within RR42_RS28295 at 1708.508 kb on - strand, within RR42_RS28295 at 1708.693 kb on + strand, within RR42_RS28295 at 1708.693 kb on + strand, within RR42_RS28295 at 1709.014 kb on + strand, within RR42_RS28295 at 1709.140 kb on + strand, within RR42_RS28295 at 1709.177 kb on + strand, within RR42_RS28295 at 1709.252 kb on + strand, within RR42_RS28295 at 1709.306 kb on + strand, within RR42_RS28295 at 1709.306 kb on + strand, within RR42_RS28295 at 1709.306 kb on + strand, within RR42_RS28295 at 1709.307 kb on - strand, within RR42_RS28295 at 1709.307 kb on - strand, within RR42_RS28295 at 1709.307 kb on - strand, within RR42_RS28295 at 1709.324 kb on + strand, within RR42_RS28295 at 1709.469 kb on - strand at 1709.530 kb on + strand at 1709.665 kb on + strand, within RR42_RS28300 at 1709.666 kb on - strand, within RR42_RS28300 at 1709.786 kb on + strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1710.049 kb on + strand, within RR42_RS28300 at 1710.049 kb on + strand, within RR42_RS28300 at 1710.050 kb on - strand, within RR42_RS28300 at 1710.050 kb on - strand, within RR42_RS28300 at 1710.050 kb on - strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.092 kb on - strand, within RR42_RS28300 at 1710.092 kb on - strand, within RR42_RS28300 at 1710.167 kb on - strand, within RR42_RS28300 at 1710.248 kb on - strand, within RR42_RS28300 at 1710.482 kb on + strand at 1710.483 kb on - strand at 1710.529 kb on - strand at 1710.530 kb on + strand at 1710.530 kb on + strand at 1710.531 kb on - strand at 1710.531 kb on - strand at 1710.553 kb on + strand at 1710.638 kb on + strand at 1710.675 kb on + strand at 1710.676 kb on - strand at 1710.676 kb on - strand at 1710.898 kb on - strand at 1710.898 kb on - strand at 1710.898 kb on - strand at 1710.909 kb on - strand at 1711.075 kb on + strand, within RR42_RS28305 at 1711.075 kb on + strand, within RR42_RS28305 at 1711.136 kb on - strand, within RR42_RS28305 at 1711.165 kb on + strand, within RR42_RS28305 at 1711.270 kb on + strand, within RR42_RS28305 at 1711.271 kb on - strand, within RR42_RS28305 at 1711.337 kb on - strand, within RR42_RS28305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 1,708,498 + RR42_RS28295 0.21 -0.1 1,708,499 - RR42_RS28295 0.21 -0.3 1,708,499 - RR42_RS28295 0.21 +0.9 1,708,508 - RR42_RS28295 0.21 -0.8 1,708,693 + RR42_RS28295 0.37 -0.5 1,708,693 + RR42_RS28295 0.37 -1.8 1,709,014 + RR42_RS28295 0.64 -0.5 1,709,140 + RR42_RS28295 0.74 +1.8 1,709,177 + RR42_RS28295 0.77 +0.2 1,709,252 + RR42_RS28295 0.83 +0.1 1,709,306 + RR42_RS28295 0.88 +1.1 1,709,306 + RR42_RS28295 0.88 -0.7 1,709,306 + RR42_RS28295 0.88 -1.0 1,709,307 - RR42_RS28295 0.88 +0.3 1,709,307 - RR42_RS28295 0.88 +0.6 1,709,307 - RR42_RS28295 0.88 -0.7 1,709,324 + RR42_RS28295 0.89 -1.0 1,709,469 - +0.4 1,709,530 + +0.3 1,709,665 + RR42_RS28300 0.22 +0.8 1,709,666 - RR42_RS28300 0.22 +0.4 1,709,786 + RR42_RS28300 0.35 +1.0 1,709,787 - RR42_RS28300 0.35 +2.1 1,709,787 - RR42_RS28300 0.35 +1.1 1,709,787 - RR42_RS28300 0.35 +1.3 1,709,787 - RR42_RS28300 0.35 -0.7 1,710,049 + RR42_RS28300 0.62 -0.7 1,710,049 + RR42_RS28300 0.62 +1.0 1,710,050 - RR42_RS28300 0.62 -1.2 1,710,050 - RR42_RS28300 0.62 +0.3 1,710,050 - RR42_RS28300 0.62 +0.1 1,710,091 + RR42_RS28300 0.66 +1.4 1,710,091 + RR42_RS28300 0.66 -0.5 1,710,091 + RR42_RS28300 0.66 +0.7 1,710,091 + RR42_RS28300 0.66 -0.0 1,710,092 - RR42_RS28300 0.66 -0.9 1,710,092 - RR42_RS28300 0.66 +0.3 1,710,167 - RR42_RS28300 0.74 +1.7 1,710,248 - RR42_RS28300 0.82 +0.1 1,710,482 + +0.2 1,710,483 - +1.3 1,710,529 - +0.3 1,710,530 + +0.4 1,710,530 + +0.5 1,710,531 - +0.6 1,710,531 - +0.7 1,710,553 + +0.7 1,710,638 + +0.3 1,710,675 + -0.4 1,710,676 - +1.4 1,710,676 - -0.7 1,710,898 - -2.2 1,710,898 - +1.1 1,710,898 - -0.5 1,710,909 - -0.3 1,711,075 + RR42_RS28305 0.12 -1.8 1,711,075 + RR42_RS28305 0.12 +0.1 1,711,136 - RR42_RS28305 0.17 -1.1 1,711,165 + RR42_RS28305 0.19 -0.7 1,711,270 + RR42_RS28305 0.26 -0.5 1,711,271 - RR42_RS28305 0.26 -1.4 1,711,337 - RR42_RS28305 0.31 +0.5
Or see this region's nucleotide sequence