Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28045 and RR42_RS28050 overlap by 8 nucleotides RR42_RS28050 and RR42_RS28055 are separated by 38 nucleotides RR42_RS28055 and RR42_RS28060 are separated by 36 nucleotides
RR42_RS28045: RR42_RS28045 - 3-oxoacyl-ACP reductase, at 1,653,590 to 1,654,339
_RS28045
RR42_RS28050: RR42_RS28050 - 3-hydroxypropionyl-CoA dehydratase, at 1,654,332 to 1,655,114
_RS28050
RR42_RS28055: RR42_RS28055 - acetyl-CoA acetyltransferase, at 1,655,153 to 1,656,349
_RS28055
RR42_RS28060: RR42_RS28060 - dehydratase, at 1,656,386 to 1,657,624
_RS28060
Position (kb)
1655
1656
1657 Strain fitness (log2 ratio)
-1
0
1 at 1654.184 kb on + strand, within RR42_RS28045 at 1654.297 kb on + strand at 1655.246 kb on - strand at 1655.519 kb on + strand, within RR42_RS28055 at 1655.519 kb on + strand, within RR42_RS28055 at 1655.519 kb on + strand, within RR42_RS28055 at 1655.519 kb on + strand, within RR42_RS28055 at 1655.520 kb on - strand, within RR42_RS28055 at 1655.522 kb on + strand, within RR42_RS28055 at 1655.523 kb on - strand, within RR42_RS28055 at 1655.523 kb on - strand, within RR42_RS28055 at 1655.523 kb on - strand, within RR42_RS28055 at 1655.669 kb on + strand, within RR42_RS28055 at 1655.995 kb on + strand, within RR42_RS28055 at 1655.995 kb on + strand, within RR42_RS28055 at 1655.995 kb on + strand, within RR42_RS28055 at 1655.995 kb on + strand, within RR42_RS28055 at 1655.995 kb on + strand, within RR42_RS28055 at 1656.392 kb on + strand at 1656.393 kb on - strand at 1656.417 kb on + strand at 1656.417 kb on + strand at 1656.436 kb on + strand at 1656.521 kb on + strand, within RR42_RS28060 at 1656.521 kb on + strand, within RR42_RS28060 at 1656.684 kb on + strand, within RR42_RS28060 at 1656.697 kb on + strand, within RR42_RS28060 at 1656.902 kb on + strand, within RR42_RS28060 at 1656.902 kb on + strand, within RR42_RS28060 at 1657.076 kb on + strand, within RR42_RS28060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 1,654,184 + RR42_RS28045 0.79 +0.4 1,654,297 + +1.4 1,655,246 - -0.1 1,655,519 + RR42_RS28055 0.31 -0.8 1,655,519 + RR42_RS28055 0.31 -0.2 1,655,519 + RR42_RS28055 0.31 -0.2 1,655,519 + RR42_RS28055 0.31 -1.4 1,655,520 - RR42_RS28055 0.31 +0.2 1,655,522 + RR42_RS28055 0.31 -0.5 1,655,523 - RR42_RS28055 0.31 -0.8 1,655,523 - RR42_RS28055 0.31 -0.2 1,655,523 - RR42_RS28055 0.31 +1.7 1,655,669 + RR42_RS28055 0.43 -0.3 1,655,995 + RR42_RS28055 0.70 -0.5 1,655,995 + RR42_RS28055 0.70 -1.5 1,655,995 + RR42_RS28055 0.70 -0.1 1,655,995 + RR42_RS28055 0.70 -0.4 1,655,995 + RR42_RS28055 0.70 -0.1 1,656,392 + -1.6 1,656,393 - +1.0 1,656,417 + +0.1 1,656,417 + -1.5 1,656,436 + -1.0 1,656,521 + RR42_RS28060 0.11 -0.2 1,656,521 + RR42_RS28060 0.11 +1.0 1,656,684 + RR42_RS28060 0.24 +0.1 1,656,697 + RR42_RS28060 0.25 +0.7 1,656,902 + RR42_RS28060 0.42 -0.2 1,656,902 + RR42_RS28060 0.42 -1.0 1,657,076 + RR42_RS28060 0.56 -1.5
Or see this region's nucleotide sequence