Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27825 and RR42_RS27830 overlap by 4 nucleotides RR42_RS27830 and RR42_RS27835 are separated by 59 nucleotides RR42_RS27835 and RR42_RS27840 are separated by 37 nucleotides RR42_RS27840 and RR42_RS27845 are separated by 65 nucleotides
RR42_RS27825: RR42_RS27825 - 4-hydroxyphenylacetate isomerase, at 1,607,156 to 1,607,887
_RS27825
RR42_RS27830: RR42_RS27830 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 1,607,884 to 1,608,648
_RS27830
RR42_RS27835: RR42_RS27835 - betaine-aldehyde dehydrogenase, at 1,608,708 to 1,610,168
_RS27835
RR42_RS27840: RR42_RS27840 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 1,610,206 to 1,611,054
_RS27840
RR42_RS27845: RR42_RS27845 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,611,120 to 1,611,509
_RS27845
Position (kb)
1608
1609
1610
1611 Strain fitness (log2 ratio)
-1
0
1
2 at 1607.838 kb on + strand at 1607.838 kb on + strand at 1607.948 kb on + strand at 1607.948 kb on + strand at 1608.043 kb on + strand, within RR42_RS27830 at 1608.043 kb on + strand, within RR42_RS27830 at 1608.044 kb on - strand, within RR42_RS27830 at 1608.138 kb on + strand, within RR42_RS27830 at 1608.138 kb on + strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.163 kb on + strand, within RR42_RS27830 at 1608.164 kb on - strand, within RR42_RS27830 at 1608.172 kb on + strand, within RR42_RS27830 at 1608.173 kb on - strand, within RR42_RS27830 at 1608.235 kb on + strand, within RR42_RS27830 at 1608.235 kb on + strand, within RR42_RS27830 at 1608.241 kb on + strand, within RR42_RS27830 at 1608.241 kb on + strand, within RR42_RS27830 at 1608.242 kb on - strand, within RR42_RS27830 at 1608.272 kb on - strand, within RR42_RS27830 at 1608.298 kb on + strand, within RR42_RS27830 at 1608.298 kb on + strand, within RR42_RS27830 at 1608.481 kb on + strand, within RR42_RS27830 at 1608.482 kb on - strand, within RR42_RS27830 at 1608.872 kb on + strand, within RR42_RS27835 at 1608.872 kb on + strand, within RR42_RS27835 at 1609.095 kb on + strand, within RR42_RS27835 at 1609.096 kb on - strand, within RR42_RS27835 at 1609.096 kb on - strand, within RR42_RS27835 at 1609.695 kb on + strand, within RR42_RS27835 at 1609.695 kb on + strand, within RR42_RS27835 at 1609.696 kb on - strand, within RR42_RS27835 at 1609.696 kb on - strand, within RR42_RS27835 at 1609.923 kb on + strand, within RR42_RS27835 at 1609.924 kb on - strand, within RR42_RS27835 at 1609.924 kb on - strand, within RR42_RS27835 at 1609.924 kb on - strand, within RR42_RS27835 at 1609.938 kb on + strand, within RR42_RS27835 at 1609.939 kb on - strand, within RR42_RS27835 at 1610.147 kb on + strand at 1610.148 kb on - strand at 1610.216 kb on + strand at 1610.216 kb on + strand at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.474 kb on - strand, within RR42_RS27840 at 1610.603 kb on - strand, within RR42_RS27840 at 1610.897 kb on - strand, within RR42_RS27840 at 1610.972 kb on - strand at 1610.973 kb on - strand at 1611.142 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 1,607,838 + +0.1 1,607,838 + -0.0 1,607,948 + -0.6 1,607,948 + -0.5 1,608,043 + RR42_RS27830 0.21 -0.1 1,608,043 + RR42_RS27830 0.21 +0.9 1,608,044 - RR42_RS27830 0.21 +0.5 1,608,138 + RR42_RS27830 0.33 -1.5 1,608,138 + RR42_RS27830 0.33 +0.0 1,608,139 - RR42_RS27830 0.33 +0.2 1,608,139 - RR42_RS27830 0.33 +0.1 1,608,139 - RR42_RS27830 0.33 +0.4 1,608,139 - RR42_RS27830 0.33 +0.7 1,608,139 - RR42_RS27830 0.33 -0.2 1,608,163 + RR42_RS27830 0.36 -0.5 1,608,164 - RR42_RS27830 0.37 -0.9 1,608,172 + RR42_RS27830 0.38 -0.2 1,608,173 - RR42_RS27830 0.38 +0.6 1,608,235 + RR42_RS27830 0.46 +1.0 1,608,235 + RR42_RS27830 0.46 +0.2 1,608,241 + RR42_RS27830 0.47 -0.4 1,608,241 + RR42_RS27830 0.47 +0.3 1,608,242 - RR42_RS27830 0.47 +0.1 1,608,272 - RR42_RS27830 0.51 +1.7 1,608,298 + RR42_RS27830 0.54 -0.3 1,608,298 + RR42_RS27830 0.54 +0.6 1,608,481 + RR42_RS27830 0.78 -0.0 1,608,482 - RR42_RS27830 0.78 -0.7 1,608,872 + RR42_RS27835 0.11 -1.4 1,608,872 + RR42_RS27835 0.11 -1.1 1,609,095 + RR42_RS27835 0.26 +0.6 1,609,096 - RR42_RS27835 0.27 -0.5 1,609,096 - RR42_RS27835 0.27 +0.1 1,609,695 + RR42_RS27835 0.68 +0.2 1,609,695 + RR42_RS27835 0.68 +0.2 1,609,696 - RR42_RS27835 0.68 -0.1 1,609,696 - RR42_RS27835 0.68 -0.9 1,609,923 + RR42_RS27835 0.83 -0.8 1,609,924 - RR42_RS27835 0.83 -0.3 1,609,924 - RR42_RS27835 0.83 -0.3 1,609,924 - RR42_RS27835 0.83 +1.1 1,609,938 + RR42_RS27835 0.84 -1.0 1,609,939 - RR42_RS27835 0.84 +1.3 1,610,147 + +0.3 1,610,148 - -0.7 1,610,216 + -0.3 1,610,216 + +0.3 1,610,431 + RR42_RS27840 0.27 +0.1 1,610,431 + RR42_RS27840 0.27 -1.1 1,610,431 + RR42_RS27840 0.27 -0.4 1,610,431 + RR42_RS27840 0.27 +0.1 1,610,474 - RR42_RS27840 0.32 -0.5 1,610,603 - RR42_RS27840 0.47 -0.7 1,610,897 - RR42_RS27840 0.81 -0.6 1,610,972 - -0.4 1,610,973 - +0.7 1,611,142 - +2.7
Or see this region's nucleotide sequence