Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27825 and RR42_RS27830 overlap by 4 nucleotides RR42_RS27830 and RR42_RS27835 are separated by 59 nucleotides
RR42_RS27825: RR42_RS27825 - 4-hydroxyphenylacetate isomerase, at 1,607,156 to 1,607,887
_RS27825
RR42_RS27830: RR42_RS27830 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 1,607,884 to 1,608,648
_RS27830
RR42_RS27835: RR42_RS27835 - betaine-aldehyde dehydrogenase, at 1,608,708 to 1,610,168
_RS27835
Position (kb)
1607
1608
1609 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1606.988 kb on + strand at 1606.989 kb on - strand at 1607.253 kb on - strand, within RR42_RS27825 at 1607.319 kb on - strand, within RR42_RS27825 at 1607.348 kb on + strand, within RR42_RS27825 at 1607.349 kb on - strand, within RR42_RS27825 at 1607.492 kb on + strand, within RR42_RS27825 at 1607.493 kb on - strand, within RR42_RS27825 at 1607.493 kb on - strand, within RR42_RS27825 at 1607.493 kb on - strand, within RR42_RS27825 at 1607.493 kb on - strand, within RR42_RS27825 at 1607.493 kb on - strand, within RR42_RS27825 at 1607.543 kb on + strand, within RR42_RS27825 at 1607.546 kb on + strand, within RR42_RS27825 at 1607.838 kb on + strand at 1607.838 kb on + strand at 1607.948 kb on + strand at 1607.948 kb on + strand at 1608.043 kb on + strand, within RR42_RS27830 at 1608.043 kb on + strand, within RR42_RS27830 at 1608.044 kb on - strand, within RR42_RS27830 at 1608.138 kb on + strand, within RR42_RS27830 at 1608.138 kb on + strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.139 kb on - strand, within RR42_RS27830 at 1608.163 kb on + strand, within RR42_RS27830 at 1608.164 kb on - strand, within RR42_RS27830 at 1608.172 kb on + strand, within RR42_RS27830 at 1608.173 kb on - strand, within RR42_RS27830 at 1608.235 kb on + strand, within RR42_RS27830 at 1608.235 kb on + strand, within RR42_RS27830 at 1608.241 kb on + strand, within RR42_RS27830 at 1608.241 kb on + strand, within RR42_RS27830 at 1608.242 kb on - strand, within RR42_RS27830 at 1608.272 kb on - strand, within RR42_RS27830 at 1608.298 kb on + strand, within RR42_RS27830 at 1608.298 kb on + strand, within RR42_RS27830 at 1608.481 kb on + strand, within RR42_RS27830 at 1608.482 kb on - strand, within RR42_RS27830 at 1608.872 kb on + strand, within RR42_RS27835 at 1608.872 kb on + strand, within RR42_RS27835 at 1609.095 kb on + strand, within RR42_RS27835 at 1609.096 kb on - strand, within RR42_RS27835 at 1609.096 kb on - strand, within RR42_RS27835
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 1,606,988 + +0.5 1,606,989 - -1.2 1,607,253 - RR42_RS27825 0.13 +1.0 1,607,319 - RR42_RS27825 0.22 +1.4 1,607,348 + RR42_RS27825 0.26 -0.3 1,607,349 - RR42_RS27825 0.26 -0.1 1,607,492 + RR42_RS27825 0.46 -0.5 1,607,493 - RR42_RS27825 0.46 -2.4 1,607,493 - RR42_RS27825 0.46 -0.3 1,607,493 - RR42_RS27825 0.46 +3.3 1,607,493 - RR42_RS27825 0.46 -0.8 1,607,493 - RR42_RS27825 0.46 +1.3 1,607,543 + RR42_RS27825 0.53 -0.7 1,607,546 + RR42_RS27825 0.53 -0.1 1,607,838 + +0.1 1,607,838 + -0.0 1,607,948 + -0.6 1,607,948 + -0.5 1,608,043 + RR42_RS27830 0.21 -0.1 1,608,043 + RR42_RS27830 0.21 +0.9 1,608,044 - RR42_RS27830 0.21 +0.5 1,608,138 + RR42_RS27830 0.33 -1.5 1,608,138 + RR42_RS27830 0.33 +0.0 1,608,139 - RR42_RS27830 0.33 +0.2 1,608,139 - RR42_RS27830 0.33 +0.1 1,608,139 - RR42_RS27830 0.33 +0.4 1,608,139 - RR42_RS27830 0.33 +0.7 1,608,139 - RR42_RS27830 0.33 -0.2 1,608,163 + RR42_RS27830 0.36 -0.5 1,608,164 - RR42_RS27830 0.37 -0.9 1,608,172 + RR42_RS27830 0.38 -0.2 1,608,173 - RR42_RS27830 0.38 +0.6 1,608,235 + RR42_RS27830 0.46 +1.0 1,608,235 + RR42_RS27830 0.46 +0.2 1,608,241 + RR42_RS27830 0.47 -0.4 1,608,241 + RR42_RS27830 0.47 +0.3 1,608,242 - RR42_RS27830 0.47 +0.1 1,608,272 - RR42_RS27830 0.51 +1.7 1,608,298 + RR42_RS27830 0.54 -0.3 1,608,298 + RR42_RS27830 0.54 +0.6 1,608,481 + RR42_RS27830 0.78 -0.0 1,608,482 - RR42_RS27830 0.78 -0.7 1,608,872 + RR42_RS27835 0.11 -1.4 1,608,872 + RR42_RS27835 0.11 -1.1 1,609,095 + RR42_RS27835 0.26 +0.6 1,609,096 - RR42_RS27835 0.27 -0.5 1,609,096 - RR42_RS27835 0.27 +0.1
Or see this region's nucleotide sequence