Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25750 and RR42_RS25755 are separated by 25 nucleotides RR42_RS25755 and RR42_RS25760 are separated by 48 nucleotides RR42_RS25760 and RR42_RS25765 are separated by 90 nucleotides RR42_RS25765 and RR42_RS25770 are separated by 198 nucleotides
RR42_RS25750: RR42_RS25750 - aromatic ring-cleaving dioxygenase, at 1,117,736 to 1,118,557
_RS25750
RR42_RS25755: RR42_RS25755 - IclR family transcriptional regulator, at 1,118,583 to 1,119,440
_RS25755
RR42_RS25760: RR42_RS25760 - salicylate hydroxylase, at 1,119,489 to 1,120,619
_RS25760
RR42_RS25765: RR42_RS25765 - hypothetical protein, at 1,120,710 to 1,121,096
_RS25765
RR42_RS25770: RR42_RS25770 - glyoxalase, at 1,121,295 to 1,121,675
_RS25770
Position (kb)
1119
1120
1121 Strain fitness (log2 ratio)
-1
0
1
2 at 1118.852 kb on - strand, within RR42_RS25755 at 1118.991 kb on - strand, within RR42_RS25755 at 1119.031 kb on + strand, within RR42_RS25755 at 1119.064 kb on + strand, within RR42_RS25755 at 1119.501 kb on + strand at 1119.502 kb on - strand at 1119.502 kb on - strand at 1119.517 kb on + strand at 1119.518 kb on - strand at 1119.518 kb on - strand at 1119.518 kb on - strand at 1119.518 kb on - strand at 1119.523 kb on + strand at 1119.523 kb on + strand at 1119.524 kb on - strand at 1119.675 kb on + strand, within RR42_RS25760 at 1119.675 kb on + strand, within RR42_RS25760 at 1119.675 kb on + strand, within RR42_RS25760 at 1119.676 kb on - strand, within RR42_RS25760 at 1119.796 kb on + strand, within RR42_RS25760 at 1119.796 kb on + strand, within RR42_RS25760 at 1119.796 kb on + strand, within RR42_RS25760 at 1119.797 kb on - strand, within RR42_RS25760 at 1119.797 kb on - strand, within RR42_RS25760 at 1119.797 kb on - strand, within RR42_RS25760 at 1119.813 kb on + strand, within RR42_RS25760 at 1119.814 kb on - strand, within RR42_RS25760 at 1119.814 kb on - strand, within RR42_RS25760 at 1119.814 kb on - strand, within RR42_RS25760 at 1119.814 kb on - strand, within RR42_RS25760 at 1119.865 kb on + strand, within RR42_RS25760 at 1119.866 kb on - strand, within RR42_RS25760 at 1119.948 kb on - strand, within RR42_RS25760 at 1120.076 kb on - strand, within RR42_RS25760 at 1120.551 kb on - strand at 1120.551 kb on - strand at 1120.551 kb on - strand at 1120.848 kb on + strand, within RR42_RS25765 at 1120.848 kb on + strand, within RR42_RS25765 at 1120.848 kb on + strand, within RR42_RS25765 at 1120.849 kb on - strand, within RR42_RS25765 at 1120.849 kb on - strand, within RR42_RS25765 at 1120.849 kb on - strand, within RR42_RS25765 at 1121.029 kb on - strand, within RR42_RS25765 at 1121.292 kb on + strand at 1121.292 kb on + strand at 1121.292 kb on + strand at 1121.292 kb on + strand at 1121.293 kb on - strand at 1121.293 kb on - strand at 1121.296 kb on + strand at 1121.297 kb on - strand at 1121.297 kb on - strand at 1121.297 kb on - strand at 1121.297 kb on - strand at 1121.300 kb on + strand at 1121.301 kb on - strand at 1121.314 kb on + strand at 1121.314 kb on + strand at 1121.314 kb on + strand at 1121.314 kb on + strand at 1121.315 kb on - strand at 1121.315 kb on - strand at 1121.360 kb on - strand, within RR42_RS25770 at 1121.360 kb on - strand, within RR42_RS25770 at 1121.373 kb on - strand, within RR42_RS25770 at 1121.491 kb on + strand, within RR42_RS25770 at 1121.492 kb on - strand, within RR42_RS25770 at 1121.502 kb on + strand, within RR42_RS25770 at 1121.546 kb on + strand, within RR42_RS25770 at 1121.564 kb on + strand, within RR42_RS25770 at 1121.614 kb on + strand, within RR42_RS25770 at 1121.614 kb on + strand, within RR42_RS25770 at 1121.615 kb on - strand, within RR42_RS25770
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 1,118,852 - RR42_RS25755 0.31 -1.6 1,118,991 - RR42_RS25755 0.48 +0.1 1,119,031 + RR42_RS25755 0.52 +1.0 1,119,064 + RR42_RS25755 0.56 +1.3 1,119,501 + +2.3 1,119,502 - -0.2 1,119,502 - +1.3 1,119,517 + +1.1 1,119,518 - -1.4 1,119,518 - -0.1 1,119,518 - -0.9 1,119,518 - +0.7 1,119,523 + -0.3 1,119,523 + -0.3 1,119,524 - +0.3 1,119,675 + RR42_RS25760 0.16 -0.2 1,119,675 + RR42_RS25760 0.16 -0.1 1,119,675 + RR42_RS25760 0.16 -1.7 1,119,676 - RR42_RS25760 0.17 +0.2 1,119,796 + RR42_RS25760 0.27 +0.1 1,119,796 + RR42_RS25760 0.27 -0.1 1,119,796 + RR42_RS25760 0.27 +1.5 1,119,797 - RR42_RS25760 0.27 +0.3 1,119,797 - RR42_RS25760 0.27 +0.4 1,119,797 - RR42_RS25760 0.27 +0.1 1,119,813 + RR42_RS25760 0.29 -1.6 1,119,814 - RR42_RS25760 0.29 -0.6 1,119,814 - RR42_RS25760 0.29 +0.5 1,119,814 - RR42_RS25760 0.29 +0.0 1,119,814 - RR42_RS25760 0.29 -0.1 1,119,865 + RR42_RS25760 0.33 +1.2 1,119,866 - RR42_RS25760 0.33 -0.0 1,119,948 - RR42_RS25760 0.41 -0.7 1,120,076 - RR42_RS25760 0.52 +0.3 1,120,551 - +0.2 1,120,551 - -0.5 1,120,551 - +0.2 1,120,848 + RR42_RS25765 0.36 +0.6 1,120,848 + RR42_RS25765 0.36 +0.5 1,120,848 + RR42_RS25765 0.36 +0.1 1,120,849 - RR42_RS25765 0.36 +0.3 1,120,849 - RR42_RS25765 0.36 +1.4 1,120,849 - RR42_RS25765 0.36 +0.8 1,121,029 - RR42_RS25765 0.82 -0.1 1,121,292 + +0.7 1,121,292 + -0.3 1,121,292 + -0.5 1,121,292 + -0.1 1,121,293 - +0.9 1,121,293 - -0.8 1,121,296 + -1.7 1,121,297 - +1.1 1,121,297 - -0.2 1,121,297 - +0.4 1,121,297 - +0.2 1,121,300 + +0.5 1,121,301 - -0.9 1,121,314 + +0.9 1,121,314 + +2.5 1,121,314 + +0.9 1,121,314 + +0.3 1,121,315 - +0.3 1,121,315 - +1.4 1,121,360 - RR42_RS25770 0.17 -1.2 1,121,360 - RR42_RS25770 0.17 -1.1 1,121,373 - RR42_RS25770 0.20 +0.6 1,121,491 + RR42_RS25770 0.51 -1.5 1,121,492 - RR42_RS25770 0.52 -1.0 1,121,502 + RR42_RS25770 0.54 +0.2 1,121,546 + RR42_RS25770 0.66 +0.5 1,121,564 + RR42_RS25770 0.71 +0.1 1,121,614 + RR42_RS25770 0.84 -0.4 1,121,614 + RR42_RS25770 0.84 +0.7 1,121,615 - RR42_RS25770 0.84 -0.6
Or see this region's nucleotide sequence