Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24265 and RR42_RS24270 are separated by 98 nucleotides RR42_RS24270 and RR42_RS24275 are separated by 108 nucleotides RR42_RS24275 and RR42_RS24280 overlap by 4 nucleotides
RR42_RS24265: RR42_RS24265 - hypothetical protein, at 771,699 to 772,415
_RS24265
RR42_RS24270: RR42_RS24270 - hypothetical protein, at 772,514 to 773,482
_RS24270
RR42_RS24275: RR42_RS24275 - hypothetical protein, at 773,591 to 774,478
_RS24275
RR42_RS24280: RR42_RS24280 - gentisate 1,2-dioxygenase, at 774,475 to 775,524
_RS24280
Position (kb) 772
773
774 Strain fitness (log2 ratio) -2
-1
0
1
2 at 771.526 kb on - strand at 771.526 kb on - strand at 771.613 kb on - strand at 771.701 kb on - strand at 771.742 kb on + strand at 771.743 kb on - strand at 771.743 kb on - strand at 771.758 kb on + strand at 771.758 kb on + strand at 771.787 kb on - strand, within RR42_RS24265 at 771.896 kb on + strand, within RR42_RS24265 at 771.955 kb on + strand, within RR42_RS24265 at 771.959 kb on - strand, within RR42_RS24265 at 771.984 kb on + strand, within RR42_RS24265 at 771.985 kb on - strand, within RR42_RS24265 at 771.985 kb on - strand, within RR42_RS24265 at 772.035 kb on + strand, within RR42_RS24265 at 772.036 kb on - strand, within RR42_RS24265 at 772.036 kb on - strand, within RR42_RS24265 at 772.198 kb on + strand, within RR42_RS24265 at 772.198 kb on + strand, within RR42_RS24265 at 772.198 kb on + strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.459 kb on + strand at 772.460 kb on - strand at 772.460 kb on - strand at 772.463 kb on + strand at 772.463 kb on + strand at 772.464 kb on - strand at 772.526 kb on + strand at 772.527 kb on - strand at 772.580 kb on - strand at 772.580 kb on - strand at 772.580 kb on - strand at 772.580 kb on - strand at 772.755 kb on + strand, within RR42_RS24270 at 772.853 kb on - strand, within RR42_RS24270 at 772.938 kb on + strand, within RR42_RS24270 at 772.938 kb on + strand, within RR42_RS24270 at 772.939 kb on - strand at 773.164 kb on - strand, within RR42_RS24270 at 773.164 kb on - strand, within RR42_RS24270 at 773.164 kb on - strand, within RR42_RS24270 at 773.230 kb on - strand, within RR42_RS24270 at 773.469 kb on - strand at 773.504 kb on - strand at 773.592 kb on + strand at 773.593 kb on - strand at 773.595 kb on + strand at 773.595 kb on + strand at 773.595 kb on + strand at 773.596 kb on - strand at 773.675 kb on - strand at 773.834 kb on + strand, within RR42_RS24275 at 773.834 kb on + strand, within RR42_RS24275 at 773.834 kb on + strand, within RR42_RS24275 at 773.835 kb on - strand, within RR42_RS24275 at 774.000 kb on + strand, within RR42_RS24275 at 774.000 kb on + strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.401 kb on + strand at 774.402 kb on - strand at 774.420 kb on + strand at 774.420 kb on + strand at 774.420 kb on + strand at 774.421 kb on - strand at 774.421 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 771,526 - +0.7 771,526 - +0.3 771,613 - +2.3 771,701 - -1.3 771,742 + -2.1 771,743 - +0.2 771,743 - -0.5 771,758 + +0.9 771,758 + -0.6 771,787 - RR42_RS24265 0.12 -0.4 771,896 + RR42_RS24265 0.27 +0.2 771,955 + RR42_RS24265 0.36 +0.3 771,959 - RR42_RS24265 0.36 -0.4 771,984 + RR42_RS24265 0.40 +0.1 771,985 - RR42_RS24265 0.40 +0.3 771,985 - RR42_RS24265 0.40 -0.4 772,035 + RR42_RS24265 0.47 +0.5 772,036 - RR42_RS24265 0.47 -0.3 772,036 - RR42_RS24265 0.47 +0.8 772,198 + RR42_RS24265 0.70 +2.3 772,198 + RR42_RS24265 0.70 +0.3 772,198 + RR42_RS24265 0.70 -2.1 772,199 - RR42_RS24265 0.70 +1.0 772,199 - RR42_RS24265 0.70 +1.0 772,199 - RR42_RS24265 0.70 -0.4 772,199 - RR42_RS24265 0.70 +0.7 772,199 - RR42_RS24265 0.70 -0.3 772,199 - RR42_RS24265 0.70 +0.4 772,459 + -0.3 772,460 - -0.3 772,460 - +0.5 772,463 + -0.4 772,463 + +0.4 772,464 - +0.0 772,526 + -0.6 772,527 - -0.1 772,580 - +2.0 772,580 - +0.8 772,580 - -0.0 772,580 - +0.7 772,755 + RR42_RS24270 0.25 +0.6 772,853 - RR42_RS24270 0.35 -0.5 772,938 + RR42_RS24270 0.44 -0.3 772,938 + RR42_RS24270 0.44 -0.4 772,939 - -0.0 773,164 - RR42_RS24270 0.67 +1.0 773,164 - RR42_RS24270 0.67 +0.6 773,164 - RR42_RS24270 0.67 -0.3 773,230 - RR42_RS24270 0.74 +0.7 773,469 - -0.5 773,504 - +0.6 773,592 + -0.3 773,593 - -1.7 773,595 + +1.1 773,595 + -0.6 773,595 + -0.4 773,596 - -0.4 773,675 - +0.2 773,834 + RR42_RS24275 0.27 +0.0 773,834 + RR42_RS24275 0.27 +0.7 773,834 + RR42_RS24275 0.27 -1.2 773,835 - RR42_RS24275 0.27 -0.7 774,000 + RR42_RS24275 0.46 -0.1 774,000 + RR42_RS24275 0.46 +1.0 774,243 - RR42_RS24275 0.73 +2.0 774,243 - RR42_RS24275 0.73 +1.1 774,243 - RR42_RS24275 0.73 +1.3 774,243 - RR42_RS24275 0.73 +0.4 774,401 + -0.7 774,402 - -0.2 774,420 + +0.5 774,420 + +2.0 774,420 + -0.8 774,421 - -0.8 774,421 - +0.1
Or see this region's nucleotide sequence