Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23800 and RR42_RS23805 are separated by 407 nucleotides RR42_RS23805 and RR42_RS23810 are separated by 30 nucleotides
RR42_RS23800: RR42_RS23800 - hypothetical protein, at 666,429 to 666,728
_RS23800
RR42_RS23805: RR42_RS23805 - hypothetical protein, at 667,136 to 669,109
_RS23805
RR42_RS23810: RR42_RS23810 - hypothetical protein, at 669,140 to 671,086
_RS23810
Position (kb)
667
668
669
670 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 666.151 kb on + strand at 666.152 kb on - strand at 666.152 kb on - strand at 666.169 kb on + strand at 666.177 kb on - strand at 666.208 kb on + strand at 666.282 kb on - strand at 666.282 kb on - strand at 666.282 kb on - strand at 666.282 kb on - strand at 666.340 kb on + strand at 666.340 kb on + strand at 666.340 kb on + strand at 666.360 kb on + strand at 666.361 kb on - strand at 666.376 kb on + strand at 666.377 kb on - strand at 666.436 kb on + strand at 666.437 kb on - strand at 666.459 kb on + strand, within RR42_RS23800 at 666.459 kb on + strand, within RR42_RS23800 at 666.460 kb on - strand, within RR42_RS23800 at 666.460 kb on - strand, within RR42_RS23800 at 666.491 kb on + strand, within RR42_RS23800 at 666.524 kb on - strand, within RR42_RS23800 at 666.524 kb on - strand, within RR42_RS23800 at 666.524 kb on - strand, within RR42_RS23800 at 666.528 kb on + strand, within RR42_RS23800 at 666.529 kb on - strand, within RR42_RS23800 at 666.529 kb on - strand, within RR42_RS23800 at 666.529 kb on - strand, within RR42_RS23800 at 666.577 kb on + strand, within RR42_RS23800 at 666.577 kb on + strand, within RR42_RS23800 at 666.577 kb on + strand, within RR42_RS23800 at 666.578 kb on - strand, within RR42_RS23800 at 666.592 kb on + strand, within RR42_RS23800 at 666.593 kb on - strand, within RR42_RS23800 at 666.685 kb on - strand, within RR42_RS23800 at 666.706 kb on - strand at 666.706 kb on - strand at 666.878 kb on - strand at 666.901 kb on - strand at 666.962 kb on - strand at 666.962 kb on - strand at 666.970 kb on + strand at 666.971 kb on - strand at 667.000 kb on + strand at 667.000 kb on + strand at 667.001 kb on - strand at 667.041 kb on + strand at 667.041 kb on + strand at 667.042 kb on - strand at 667.042 kb on - strand at 667.042 kb on - strand at 667.051 kb on + strand at 667.051 kb on + strand at 667.051 kb on + strand at 667.052 kb on - strand at 667.052 kb on - strand at 667.052 kb on - strand at 667.057 kb on + strand at 667.057 kb on + strand at 667.057 kb on + strand at 667.058 kb on - strand at 667.058 kb on - strand at 667.058 kb on - strand at 667.058 kb on - strand at 667.058 kb on - strand at 667.288 kb on - strand at 667.288 kb on - strand at 667.288 kb on - strand at 667.513 kb on + strand, within RR42_RS23805 at 667.513 kb on + strand, within RR42_RS23805 at 667.659 kb on - strand, within RR42_RS23805 at 667.736 kb on - strand, within RR42_RS23805 at 667.765 kb on - strand, within RR42_RS23805 at 667.765 kb on - strand, within RR42_RS23805 at 667.765 kb on - strand, within RR42_RS23805 at 667.857 kb on + strand, within RR42_RS23805 at 667.857 kb on + strand, within RR42_RS23805 at 667.857 kb on + strand, within RR42_RS23805 at 667.858 kb on - strand, within RR42_RS23805 at 667.858 kb on - strand, within RR42_RS23805 at 667.926 kb on + strand, within RR42_RS23805 at 668.011 kb on - strand, within RR42_RS23805 at 668.170 kb on - strand, within RR42_RS23805 at 668.170 kb on - strand, within RR42_RS23805 at 668.227 kb on - strand, within RR42_RS23805 at 668.227 kb on - strand, within RR42_RS23805 at 668.266 kb on - strand, within RR42_RS23805 at 668.266 kb on - strand, within RR42_RS23805 at 668.266 kb on - strand, within RR42_RS23805 at 668.266 kb on - strand, within RR42_RS23805 at 668.266 kb on - strand, within RR42_RS23805 at 668.277 kb on + strand, within RR42_RS23805 at 668.277 kb on + strand, within RR42_RS23805 at 668.278 kb on - strand, within RR42_RS23805 at 668.436 kb on + strand, within RR42_RS23805 at 668.436 kb on + strand, within RR42_RS23805 at 668.514 kb on + strand, within RR42_RS23805 at 668.514 kb on + strand, within RR42_RS23805 at 668.514 kb on + strand, within RR42_RS23805 at 668.515 kb on - strand, within RR42_RS23805 at 668.647 kb on + strand, within RR42_RS23805 at 668.648 kb on - strand, within RR42_RS23805 at 668.648 kb on - strand, within RR42_RS23805 at 668.760 kb on + strand, within RR42_RS23805 at 668.760 kb on + strand, within RR42_RS23805 at 668.761 kb on - strand, within RR42_RS23805 at 668.761 kb on - strand, within RR42_RS23805 at 668.848 kb on + strand, within RR42_RS23805 at 668.848 kb on + strand, within RR42_RS23805 at 668.849 kb on - strand, within RR42_RS23805 at 668.862 kb on - strand, within RR42_RS23805 at 668.884 kb on - strand, within RR42_RS23805 at 668.884 kb on - strand, within RR42_RS23805 at 668.961 kb on + strand at 668.962 kb on - strand at 669.086 kb on + strand at 669.103 kb on - strand at 669.103 kb on - strand at 669.181 kb on - strand at 669.215 kb on - strand at 669.300 kb on + strand at 669.301 kb on - strand at 669.361 kb on - strand, within RR42_RS23810 at 669.482 kb on + strand, within RR42_RS23810 at 669.482 kb on + strand, within RR42_RS23810 at 669.483 kb on - strand, within RR42_RS23810 at 669.520 kb on + strand, within RR42_RS23810 at 669.520 kb on + strand, within RR42_RS23810 at 669.521 kb on - strand, within RR42_RS23810
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 666,151 + +0.1 666,152 - -0.6 666,152 - -1.3 666,169 + -1.3 666,177 - +1.5 666,208 + -0.5 666,282 - +0.1 666,282 - -0.4 666,282 - +0.2 666,282 - -0.4 666,340 + +0.8 666,340 + -0.3 666,340 + +0.5 666,360 + -0.5 666,361 - -0.7 666,376 + -0.9 666,377 - +0.2 666,436 + -1.8 666,437 - -1.0 666,459 + RR42_RS23800 0.10 -0.7 666,459 + RR42_RS23800 0.10 -0.2 666,460 - RR42_RS23800 0.10 -1.0 666,460 - RR42_RS23800 0.10 -0.9 666,491 + RR42_RS23800 0.21 -0.6 666,524 - RR42_RS23800 0.32 +1.1 666,524 - RR42_RS23800 0.32 -0.8 666,524 - RR42_RS23800 0.32 -1.0 666,528 + RR42_RS23800 0.33 +0.4 666,529 - RR42_RS23800 0.33 +1.4 666,529 - RR42_RS23800 0.33 -0.7 666,529 - RR42_RS23800 0.33 -0.4 666,577 + RR42_RS23800 0.49 -0.5 666,577 + RR42_RS23800 0.49 +0.9 666,577 + RR42_RS23800 0.49 +1.4 666,578 - RR42_RS23800 0.50 -0.3 666,592 + RR42_RS23800 0.54 +0.7 666,593 - RR42_RS23800 0.55 -0.7 666,685 - RR42_RS23800 0.85 +0.3 666,706 - +0.9 666,706 - -0.7 666,878 - +0.6 666,901 - -1.2 666,962 - -0.6 666,962 - -2.2 666,970 + -0.5 666,971 - +0.3 667,000 + +1.3 667,000 + +0.9 667,001 - +0.5 667,041 + +0.4 667,041 + -0.7 667,042 - -0.8 667,042 - +0.3 667,042 - -0.8 667,051 + +1.3 667,051 + +0.1 667,051 + -0.1 667,052 - +0.1 667,052 - +0.0 667,052 - -0.3 667,057 + +0.6 667,057 + +0.4 667,057 + +0.2 667,058 - +1.1 667,058 - -0.5 667,058 - -0.1 667,058 - -0.9 667,058 - +0.5 667,288 - -1.6 667,288 - +0.5 667,288 - -0.1 667,513 + RR42_RS23805 0.19 -0.6 667,513 + RR42_RS23805 0.19 -0.7 667,659 - RR42_RS23805 0.26 -1.0 667,736 - RR42_RS23805 0.30 +1.0 667,765 - RR42_RS23805 0.32 -0.0 667,765 - RR42_RS23805 0.32 -1.5 667,765 - RR42_RS23805 0.32 +1.0 667,857 + RR42_RS23805 0.37 -0.4 667,857 + RR42_RS23805 0.37 -1.1 667,857 + RR42_RS23805 0.37 +0.2 667,858 - RR42_RS23805 0.37 +0.6 667,858 - RR42_RS23805 0.37 +0.7 667,926 + RR42_RS23805 0.40 +2.0 668,011 - RR42_RS23805 0.44 +2.0 668,170 - RR42_RS23805 0.52 +0.1 668,170 - RR42_RS23805 0.52 +0.7 668,227 - RR42_RS23805 0.55 +1.1 668,227 - RR42_RS23805 0.55 -0.5 668,266 - RR42_RS23805 0.57 -0.7 668,266 - RR42_RS23805 0.57 +2.3 668,266 - RR42_RS23805 0.57 +1.0 668,266 - RR42_RS23805 0.57 +0.4 668,266 - RR42_RS23805 0.57 +0.1 668,277 + RR42_RS23805 0.58 -0.3 668,277 + RR42_RS23805 0.58 -0.4 668,278 - RR42_RS23805 0.58 +0.5 668,436 + RR42_RS23805 0.66 -0.2 668,436 + RR42_RS23805 0.66 -0.5 668,514 + RR42_RS23805 0.70 +1.0 668,514 + RR42_RS23805 0.70 -1.6 668,514 + RR42_RS23805 0.70 -0.0 668,515 - RR42_RS23805 0.70 +1.0 668,647 + RR42_RS23805 0.77 +0.0 668,648 - RR42_RS23805 0.77 +1.1 668,648 - RR42_RS23805 0.77 -0.6 668,760 + RR42_RS23805 0.82 +1.1 668,760 + RR42_RS23805 0.82 -0.9 668,761 - RR42_RS23805 0.82 -0.4 668,761 - RR42_RS23805 0.82 -0.0 668,848 + RR42_RS23805 0.87 +0.2 668,848 + RR42_RS23805 0.87 +1.1 668,849 - RR42_RS23805 0.87 +0.0 668,862 - RR42_RS23805 0.87 -0.5 668,884 - RR42_RS23805 0.89 -0.9 668,884 - RR42_RS23805 0.89 +0.2 668,961 + -0.1 668,962 - -0.7 669,086 + +2.0 669,103 - -0.5 669,103 - -0.3 669,181 - -0.5 669,215 - -0.2 669,300 + -1.3 669,301 - -0.3 669,361 - RR42_RS23810 0.11 +1.1 669,482 + RR42_RS23810 0.18 +0.1 669,482 + RR42_RS23810 0.18 -1.4 669,483 - RR42_RS23810 0.18 +0.4 669,520 + RR42_RS23810 0.20 +0.8 669,520 + RR42_RS23810 0.20 -0.7 669,521 - RR42_RS23810 0.20 +0.5
Or see this region's nucleotide sequence