Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22765 and RR42_RS22770 are separated by 56 nucleotides RR42_RS22770 and RR42_RS22775 are separated by 2 nucleotides RR42_RS22775 and RR42_RS22780 are separated by 20 nucleotides
RR42_RS22765: RR42_RS22765 - carboxyvinyl-carboxyphosphonate phosphorylmutase, at 440,242 to 441,084
_RS22765
RR42_RS22770: RR42_RS22770 - 3-isopropylmalate dehydratase, at 441,141 to 442,541
_RS22770
RR42_RS22775: RR42_RS22775 - 3-isopropylmalate dehydratase, at 442,544 to 443,170
_RS22775
RR42_RS22780: RR42_RS22780 - hypothetical protein, at 443,191 to 443,745
_RS22780
Position (kb) 441
442
443 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 440.194 kb on + strand at 440.194 kb on + strand at 440.194 kb on + strand at 440.256 kb on + strand at 440.256 kb on + strand at 440.257 kb on - strand at 440.257 kb on - strand at 440.257 kb on - strand at 440.308 kb on + strand at 440.308 kb on + strand at 440.359 kb on + strand, within RR42_RS22765 at 440.359 kb on + strand, within RR42_RS22765 at 440.360 kb on - strand, within RR42_RS22765 at 440.360 kb on - strand, within RR42_RS22765 at 440.536 kb on + strand, within RR42_RS22765 at 440.536 kb on + strand, within RR42_RS22765 at 440.915 kb on - strand, within RR42_RS22765 at 440.915 kb on - strand, within RR42_RS22765 at 441.292 kb on + strand, within RR42_RS22770 at 441.292 kb on + strand, within RR42_RS22770 at 441.293 kb on - strand, within RR42_RS22770 at 441.293 kb on - strand, within RR42_RS22770 at 441.293 kb on - strand, within RR42_RS22770 at 441.363 kb on - strand, within RR42_RS22770 at 441.530 kb on + strand, within RR42_RS22770 at 441.530 kb on + strand, within RR42_RS22770 at 441.530 kb on + strand, within RR42_RS22770 at 441.530 kb on + strand, within RR42_RS22770 at 441.536 kb on + strand, within RR42_RS22770 at 441.588 kb on - strand, within RR42_RS22770 at 441.588 kb on - strand, within RR42_RS22770 at 441.698 kb on + strand, within RR42_RS22770 at 441.699 kb on - strand, within RR42_RS22770 at 441.882 kb on + strand, within RR42_RS22770 at 442.113 kb on + strand, within RR42_RS22770 at 442.113 kb on + strand, within RR42_RS22770 at 442.114 kb on - strand, within RR42_RS22770 at 442.114 kb on - strand, within RR42_RS22770 at 442.114 kb on - strand, within RR42_RS22770 at 442.114 kb on - strand, within RR42_RS22770 at 442.525 kb on + strand at 442.556 kb on - strand at 442.850 kb on + strand, within RR42_RS22775 at 442.851 kb on - strand, within RR42_RS22775 at 442.851 kb on - strand, within RR42_RS22775 at 442.866 kb on - strand, within RR42_RS22775 at 442.866 kb on - strand, within RR42_RS22775 at 442.866 kb on - strand, within RR42_RS22775
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 440,194 + +1.1 440,194 + -0.6 440,194 + +1.7 440,256 + -0.7 440,256 + +1.8 440,257 - -0.1 440,257 - +2.7 440,257 - -0.8 440,308 + +0.2 440,308 + -2.0 440,359 + RR42_RS22765 0.14 -0.6 440,359 + RR42_RS22765 0.14 -1.0 440,360 - RR42_RS22765 0.14 +0.7 440,360 - RR42_RS22765 0.14 +1.1 440,536 + RR42_RS22765 0.35 +0.5 440,536 + RR42_RS22765 0.35 -0.2 440,915 - RR42_RS22765 0.80 +0.5 440,915 - RR42_RS22765 0.80 -0.8 441,292 + RR42_RS22770 0.11 +1.2 441,292 + RR42_RS22770 0.11 -0.0 441,293 - RR42_RS22770 0.11 +0.7 441,293 - RR42_RS22770 0.11 -0.2 441,293 - RR42_RS22770 0.11 +1.1 441,363 - RR42_RS22770 0.16 -0.6 441,530 + RR42_RS22770 0.28 +0.8 441,530 + RR42_RS22770 0.28 +0.5 441,530 + RR42_RS22770 0.28 +2.1 441,530 + RR42_RS22770 0.28 +0.4 441,536 + RR42_RS22770 0.28 +0.3 441,588 - RR42_RS22770 0.32 -0.2 441,588 - RR42_RS22770 0.32 +2.1 441,698 + RR42_RS22770 0.40 -0.7 441,699 - RR42_RS22770 0.40 +0.3 441,882 + RR42_RS22770 0.53 +0.1 442,113 + RR42_RS22770 0.69 +0.0 442,113 + RR42_RS22770 0.69 +0.6 442,114 - RR42_RS22770 0.69 +0.9 442,114 - RR42_RS22770 0.69 -0.1 442,114 - RR42_RS22770 0.69 -0.5 442,114 - RR42_RS22770 0.69 +1.1 442,525 + -0.6 442,556 - +1.1 442,850 + RR42_RS22775 0.49 +3.1 442,851 - RR42_RS22775 0.49 +0.1 442,851 - RR42_RS22775 0.49 -0.2 442,866 - RR42_RS22775 0.51 +2.1 442,866 - RR42_RS22775 0.51 +0.1 442,866 - RR42_RS22775 0.51 +0.1
Or see this region's nucleotide sequence