Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22290 and RR42_RS22295 are separated by 50 nucleotides RR42_RS22295 and RR42_RS22300 are separated by 226 nucleotides RR42_RS22300 and RR42_RS22305 are separated by 20 nucleotides
RR42_RS22290: RR42_RS22290 - cytochrome C biogenesis protein, at 341,415 to 343,223
_RS22290
RR42_RS22295: RR42_RS22295 - methionine sulfoxide reductase B, at 343,274 to 343,813
_RS22295
RR42_RS22300: RR42_RS22300 - chemotaxis protein CheY, at 344,040 to 344,762
_RS22300
RR42_RS22305: RR42_RS22305 - histidine kinase, at 344,783 to 346,276
_RS22305
Position (kb) 344
345 Strain fitness (log2 ratio) -2
-1
0
1
2 at 343.202 kb on - strand at 343.211 kb on + strand at 343.211 kb on + strand at 343.436 kb on + strand, within RR42_RS22295 at 343.659 kb on - strand, within RR42_RS22295 at 343.835 kb on + strand at 343.835 kb on + strand at 343.893 kb on + strand at 343.894 kb on - strand at 343.894 kb on - strand at 343.934 kb on + strand at 343.972 kb on - strand at 344.095 kb on + strand at 344.095 kb on + strand at 344.096 kb on - strand at 344.096 kb on - strand at 344.124 kb on + strand, within RR42_RS22300 at 344.125 kb on - strand, within RR42_RS22300 at 344.265 kb on + strand, within RR42_RS22300 at 344.266 kb on - strand, within RR42_RS22300 at 344.343 kb on + strand, within RR42_RS22300 at 344.343 kb on + strand, within RR42_RS22300 at 344.344 kb on - strand, within RR42_RS22300 at 344.544 kb on + strand, within RR42_RS22300 at 344.633 kb on + strand, within RR42_RS22300 at 344.633 kb on + strand, within RR42_RS22300 at 344.634 kb on - strand, within RR42_RS22300 at 344.634 kb on - strand, within RR42_RS22300 at 344.648 kb on + strand, within RR42_RS22300 at 344.648 kb on + strand, within RR42_RS22300 at 344.648 kb on + strand, within RR42_RS22300 at 344.724 kb on + strand at 344.724 kb on + strand at 344.725 kb on - strand at 345.034 kb on - strand, within RR42_RS22305 at 345.041 kb on + strand, within RR42_RS22305 at 345.041 kb on + strand, within RR42_RS22305 at 345.042 kb on - strand, within RR42_RS22305 at 345.244 kb on + strand, within RR42_RS22305 at 345.245 kb on + strand, within RR42_RS22305 at 345.251 kb on + strand, within RR42_RS22305 at 345.251 kb on + strand, within RR42_RS22305 at 345.251 kb on + strand, within RR42_RS22305 at 345.251 kb on + strand, within RR42_RS22305 at 345.252 kb on - strand, within RR42_RS22305 at 345.252 kb on - strand, within RR42_RS22305 at 345.252 kb on - strand, within RR42_RS22305 at 345.607 kb on + strand, within RR42_RS22305 at 345.651 kb on - strand, within RR42_RS22305 at 345.708 kb on - strand, within RR42_RS22305 at 345.708 kb on - strand, within RR42_RS22305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 343,202 - -1.6 343,211 + -0.6 343,211 + +0.1 343,436 + RR42_RS22295 0.30 -2.1 343,659 - RR42_RS22295 0.71 +1.7 343,835 + +0.7 343,835 + +0.2 343,893 + -0.4 343,894 - +2.1 343,894 - -0.2 343,934 + -1.0 343,972 - +1.3 344,095 + -1.4 344,095 + +0.5 344,096 - -0.0 344,096 - -0.2 344,124 + RR42_RS22300 0.12 -0.5 344,125 - RR42_RS22300 0.12 +0.4 344,265 + RR42_RS22300 0.31 +0.3 344,266 - RR42_RS22300 0.31 -0.9 344,343 + RR42_RS22300 0.42 +0.3 344,343 + RR42_RS22300 0.42 -0.8 344,344 - RR42_RS22300 0.42 +2.7 344,544 + RR42_RS22300 0.70 +0.5 344,633 + RR42_RS22300 0.82 +0.5 344,633 + RR42_RS22300 0.82 +0.5 344,634 - RR42_RS22300 0.82 -0.1 344,634 - RR42_RS22300 0.82 +0.9 344,648 + RR42_RS22300 0.84 +0.9 344,648 + RR42_RS22300 0.84 +1.1 344,648 + RR42_RS22300 0.84 +0.4 344,724 + -0.1 344,724 + +0.8 344,725 - +0.6 345,034 - RR42_RS22305 0.17 +0.2 345,041 + RR42_RS22305 0.17 -2.3 345,041 + RR42_RS22305 0.17 -1.5 345,042 - RR42_RS22305 0.17 -0.2 345,244 + RR42_RS22305 0.31 -0.1 345,245 + RR42_RS22305 0.31 -2.1 345,251 + RR42_RS22305 0.31 -0.7 345,251 + RR42_RS22305 0.31 -0.1 345,251 + RR42_RS22305 0.31 -1.5 345,251 + RR42_RS22305 0.31 -0.0 345,252 - RR42_RS22305 0.31 +0.2 345,252 - RR42_RS22305 0.31 +0.2 345,252 - RR42_RS22305 0.31 -0.2 345,607 + RR42_RS22305 0.55 +0.4 345,651 - RR42_RS22305 0.58 -2.2 345,708 - RR42_RS22305 0.62 -0.8 345,708 - RR42_RS22305 0.62 -0.9
Or see this region's nucleotide sequence