Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22245 and RR42_RS22250 are separated by 25 nucleotides RR42_RS22250 and RR42_RS22255 are separated by 23 nucleotides
RR42_RS22245: RR42_RS22245 - cytochrome C biogenesis protein, at 329,373 to 330,320
_RS22245
RR42_RS22250: RR42_RS22250 - hypothetical protein, at 330,346 to 330,642
_RS22250
RR42_RS22255: RR42_RS22255 - hypothetical protein, at 330,666 to 332,096
_RS22255
Position (kb)
330
331 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 329.379 kb on + strand at 329.380 kb on - strand at 329.391 kb on + strand at 329.392 kb on - strand at 329.466 kb on + strand at 329.467 kb on - strand at 329.467 kb on - strand at 329.529 kb on + strand, within RR42_RS22245 at 329.530 kb on - strand, within RR42_RS22245 at 329.587 kb on - strand, within RR42_RS22245 at 329.640 kb on + strand, within RR42_RS22245 at 329.640 kb on + strand, within RR42_RS22245 at 329.834 kb on + strand, within RR42_RS22245 at 329.834 kb on + strand, within RR42_RS22245 at 329.983 kb on - strand, within RR42_RS22245 at 330.284 kb on + strand at 330.284 kb on + strand at 330.341 kb on + strand at 330.342 kb on - strand at 330.609 kb on - strand, within RR42_RS22250 at 330.718 kb on + strand at 330.718 kb on + strand at 330.718 kb on + strand at 330.718 kb on + strand at 330.719 kb on - strand at 330.719 kb on - strand at 330.719 kb on - strand at 330.947 kb on - strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.333 kb on - strand, within RR42_RS22255 at 331.333 kb on - strand, within RR42_RS22255 at 331.544 kb on - strand, within RR42_RS22255 at 331.544 kb on - strand, within RR42_RS22255 at 331.564 kb on + strand, within RR42_RS22255 at 331.565 kb on - strand, within RR42_RS22255 at 331.565 kb on - strand, within RR42_RS22255 at 331.631 kb on - strand, within RR42_RS22255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 329,379 + -0.5 329,380 - -0.1 329,391 + +0.5 329,392 - +0.7 329,466 + -0.9 329,467 - +0.5 329,467 - +1.4 329,529 + RR42_RS22245 0.16 +1.6 329,530 - RR42_RS22245 0.17 +0.2 329,587 - RR42_RS22245 0.23 -0.6 329,640 + RR42_RS22245 0.28 +2.4 329,640 + RR42_RS22245 0.28 -0.2 329,834 + RR42_RS22245 0.49 -1.1 329,834 + RR42_RS22245 0.49 -1.5 329,983 - RR42_RS22245 0.64 -0.2 330,284 + -0.0 330,284 + -0.7 330,341 + +1.0 330,342 - +0.3 330,609 - RR42_RS22250 0.89 -0.5 330,718 + +0.3 330,718 + -1.4 330,718 + -0.9 330,718 + +0.2 330,719 - +1.1 330,719 - +0.2 330,719 - -1.0 330,947 - RR42_RS22255 0.20 -0.2 331,332 + RR42_RS22255 0.47 -0.3 331,332 + RR42_RS22255 0.47 +0.6 331,332 + RR42_RS22255 0.47 -0.9 331,333 - RR42_RS22255 0.47 -0.1 331,333 - RR42_RS22255 0.47 -0.6 331,544 - RR42_RS22255 0.61 -0.8 331,544 - RR42_RS22255 0.61 +1.1 331,564 + RR42_RS22255 0.63 +0.6 331,565 - RR42_RS22255 0.63 -1.8 331,565 - RR42_RS22255 0.63 -0.6 331,631 - RR42_RS22255 0.67 -1.0
Or see this region's nucleotide sequence