Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22235 and RR42_RS22240 are separated by 821 nucleotides RR42_RS22240 and RR42_RS22245 are separated by 364 nucleotides
RR42_RS22235: RR42_RS22235 - peptidase M19, at 326,256 to 326,885
_RS22235
RR42_RS22240: RR42_RS22240 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 327,707 to 329,008
_RS22240
RR42_RS22245: RR42_RS22245 - cytochrome C biogenesis protein, at 329,373 to 330,320
_RS22245
Position (kb)
327
328
329
330 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 326.827 kb on + strand at 326.828 kb on - strand at 326.828 kb on - strand at 327.005 kb on - strand at 327.651 kb on + strand at 327.652 kb on - strand at 327.652 kb on - strand at 327.697 kb on - strand at 327.751 kb on + strand at 327.752 kb on - strand at 327.752 kb on - strand at 327.752 kb on - strand at 327.755 kb on - strand at 327.902 kb on - strand, within RR42_RS22240 at 327.902 kb on - strand, within RR42_RS22240 at 327.902 kb on - strand, within RR42_RS22240 at 328.196 kb on + strand, within RR42_RS22240 at 328.196 kb on + strand, within RR42_RS22240 at 328.197 kb on - strand, within RR42_RS22240 at 328.197 kb on - strand, within RR42_RS22240 at 328.724 kb on + strand, within RR42_RS22240 at 328.850 kb on + strand, within RR42_RS22240 at 329.007 kb on - strand at 329.024 kb on + strand at 329.176 kb on + strand at 329.177 kb on - strand at 329.177 kb on - strand at 329.177 kb on - strand at 329.177 kb on - strand at 329.197 kb on - strand at 329.228 kb on + strand at 329.228 kb on + strand at 329.295 kb on + strand at 329.295 kb on + strand at 329.296 kb on - strand at 329.297 kb on + strand at 329.297 kb on + strand at 329.297 kb on + strand at 329.297 kb on + strand at 329.298 kb on - strand at 329.298 kb on - strand at 329.301 kb on + strand at 329.302 kb on - strand at 329.302 kb on - strand at 329.302 kb on - strand at 329.379 kb on + strand at 329.380 kb on - strand at 329.391 kb on + strand at 329.392 kb on - strand at 329.466 kb on + strand at 329.467 kb on - strand at 329.467 kb on - strand at 329.529 kb on + strand, within RR42_RS22245 at 329.530 kb on - strand, within RR42_RS22245 at 329.587 kb on - strand, within RR42_RS22245 at 329.640 kb on + strand, within RR42_RS22245 at 329.640 kb on + strand, within RR42_RS22245 at 329.834 kb on + strand, within RR42_RS22245 at 329.834 kb on + strand, within RR42_RS22245 at 329.983 kb on - strand, within RR42_RS22245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 326,827 + +2.1 326,828 - -0.5 326,828 - +0.1 327,005 - -0.1 327,651 + -1.2 327,652 - -0.2 327,652 - -0.1 327,697 - +0.5 327,751 + +0.5 327,752 - +1.8 327,752 - -0.5 327,752 - +0.3 327,755 - +1.8 327,902 - RR42_RS22240 0.15 +1.1 327,902 - RR42_RS22240 0.15 +1.2 327,902 - RR42_RS22240 0.15 -1.1 328,196 + RR42_RS22240 0.38 +1.0 328,196 + RR42_RS22240 0.38 +1.1 328,197 - RR42_RS22240 0.38 -1.6 328,197 - RR42_RS22240 0.38 -0.1 328,724 + RR42_RS22240 0.78 -2.8 328,850 + RR42_RS22240 0.88 +0.8 329,007 - -0.7 329,024 + -0.3 329,176 + +0.5 329,177 - +1.1 329,177 - +0.2 329,177 - -0.5 329,177 - -0.8 329,197 - +0.1 329,228 + +0.4 329,228 + +0.5 329,295 + -0.6 329,295 + -0.9 329,296 - +0.2 329,297 + +2.1 329,297 + -2.5 329,297 + -1.1 329,297 + -0.2 329,298 - +0.2 329,298 - +0.5 329,301 + -0.9 329,302 - -1.3 329,302 - -0.1 329,302 - +0.7 329,379 + -0.5 329,380 - -0.1 329,391 + +0.5 329,392 - +0.7 329,466 + -0.9 329,467 - +0.5 329,467 - +1.4 329,529 + RR42_RS22245 0.16 +1.6 329,530 - RR42_RS22245 0.17 +0.2 329,587 - RR42_RS22245 0.23 -0.6 329,640 + RR42_RS22245 0.28 +2.4 329,640 + RR42_RS22245 0.28 -0.2 329,834 + RR42_RS22245 0.49 -1.1 329,834 + RR42_RS22245 0.49 -1.5 329,983 - RR42_RS22245 0.64 -0.2
Or see this region's nucleotide sequence