Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21075 and RR42_RS21080 are separated by 40 nucleotides RR42_RS21080 and RR42_RS21085 overlap by 4 nucleotides
RR42_RS21075: RR42_RS21075 - phosphoribosyltransferase, at 54,061 to 54,768
_RS21075
RR42_RS21080: RR42_RS21080 - 3-isopropylmalate dehydratase, at 54,809 to 55,321
_RS21080
RR42_RS21085: RR42_RS21085 - 3-isopropylmalate dehydratase, at 55,318 to 56,625
_RS21085
Position (kb) 54
55
56 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 53.947 kb on + strand at 53.947 kb on - strand at 53.948 kb on - strand at 53.949 kb on + strand at 53.949 kb on + strand at 53.949 kb on + strand at 53.950 kb on - strand at 53.950 kb on - strand at 53.950 kb on - strand at 53.950 kb on - strand at 54.691 kb on + strand, within RR42_RS21075 at 55.126 kb on + strand, within RR42_RS21080 at 55.228 kb on + strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.697 kb on + strand, within RR42_RS21085 at 55.697 kb on + strand, within RR42_RS21085 at 56.012 kb on + strand, within RR42_RS21085 at 56.012 kb on + strand, within RR42_RS21085 at 56.012 kb on + strand, within RR42_RS21085 at 56.013 kb on - strand, within RR42_RS21085 at 56.027 kb on + strand, within RR42_RS21085 at 56.028 kb on - strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.127 kb on + strand, within RR42_RS21085 at 56.128 kb on - strand, within RR42_RS21085 at 56.199 kb on + strand, within RR42_RS21085 at 56.199 kb on + strand, within RR42_RS21085 at 56.199 kb on + strand, within RR42_RS21085 at 56.200 kb on - strand, within RR42_RS21085 at 56.200 kb on - strand, within RR42_RS21085 at 56.201 kb on + strand, within RR42_RS21085 at 56.201 kb on + strand, within RR42_RS21085 at 56.202 kb on - strand, within RR42_RS21085 at 56.202 kb on - strand, within RR42_RS21085 at 56.287 kb on + strand, within RR42_RS21085 at 56.287 kb on + strand, within RR42_RS21085 at 56.288 kb on - strand, within RR42_RS21085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 53,947 + -0.2 53,947 - -0.2 53,948 - -0.7 53,949 + -0.7 53,949 + -1.7 53,949 + +0.3 53,950 - +1.3 53,950 - -0.0 53,950 - -1.4 53,950 - +0.3 54,691 + RR42_RS21075 0.89 +0.4 55,126 + RR42_RS21080 0.62 -0.7 55,228 + RR42_RS21080 0.82 +0.6 55,229 - RR42_RS21080 0.82 -1.2 55,229 - RR42_RS21080 0.82 -2.5 55,229 - RR42_RS21080 0.82 -0.8 55,229 - RR42_RS21080 0.82 -1.1 55,606 + RR42_RS21085 0.22 -0.3 55,606 + RR42_RS21085 0.22 +0.1 55,606 + RR42_RS21085 0.22 -0.6 55,606 + RR42_RS21085 0.22 +0.1 55,607 - RR42_RS21085 0.22 +0.4 55,607 - RR42_RS21085 0.22 +0.6 55,607 - RR42_RS21085 0.22 +0.4 55,697 + RR42_RS21085 0.29 -1.0 55,697 + RR42_RS21085 0.29 -0.5 56,012 + RR42_RS21085 0.53 +1.0 56,012 + RR42_RS21085 0.53 -0.5 56,012 + RR42_RS21085 0.53 +1.2 56,013 - RR42_RS21085 0.53 +0.7 56,027 + RR42_RS21085 0.54 -0.2 56,028 - RR42_RS21085 0.54 +1.4 56,127 + RR42_RS21085 0.62 +0.3 56,127 + RR42_RS21085 0.62 -0.9 56,127 + RR42_RS21085 0.62 +0.3 56,127 + RR42_RS21085 0.62 +0.0 56,127 + RR42_RS21085 0.62 -0.2 56,127 + RR42_RS21085 0.62 +0.7 56,127 + RR42_RS21085 0.62 -1.3 56,128 - RR42_RS21085 0.62 -1.4 56,199 + RR42_RS21085 0.67 -0.0 56,199 + RR42_RS21085 0.67 -0.8 56,199 + RR42_RS21085 0.67 -1.7 56,200 - RR42_RS21085 0.67 +0.2 56,200 - RR42_RS21085 0.67 -0.2 56,201 + RR42_RS21085 0.68 -1.2 56,201 + RR42_RS21085 0.68 -3.0 56,202 - RR42_RS21085 0.68 -0.4 56,202 - RR42_RS21085 0.68 -1.7 56,287 + RR42_RS21085 0.74 -0.6 56,287 + RR42_RS21085 0.74 -0.2 56,288 - RR42_RS21085 0.74 -0.0
Or see this region's nucleotide sequence