Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS20970

Experiment: R2A with copper (II) chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS20965 and RR42_RS20970 are separated by 63 nucleotidesRR42_RS20970 and RR42_RS20975 overlap by 4 nucleotides RR42_RS20965: RR42_RS20965 - transferase, at 30,936 to 32,123 _RS20965 RR42_RS20970: RR42_RS20970 - hypothetical protein, at 32,187 to 33,467 _RS20970 RR42_RS20975: RR42_RS20975 - hypothetical protein, at 33,464 to 34,282 _RS20975 Position (kb) 32 33 34Strain fitness (log2 ratio) -1 0 1 2at 31.438 kb on - strand, within RR42_RS20965at 31.881 kb on - strand, within RR42_RS20965at 31.881 kb on - strand, within RR42_RS20965at 32.122 kb on - strandat 32.212 kb on - strandat 32.292 kb on + strandat 32.293 kb on - strandat 32.556 kb on + strand, within RR42_RS20970at 32.676 kb on + strand, within RR42_RS20970at 32.776 kb on + strand, within RR42_RS20970at 32.826 kb on + strand, within RR42_RS20970at 32.826 kb on + strand, within RR42_RS20970at 32.827 kb on - strand, within RR42_RS20970at 32.827 kb on - strand, within RR42_RS20970at 32.827 kb on - strand, within RR42_RS20970at 32.871 kb on + strand, within RR42_RS20970at 32.872 kb on - strand, within RR42_RS20970at 33.000 kb on + strand, within RR42_RS20970at 33.122 kb on - strand, within RR42_RS20970at 33.122 kb on - strand, within RR42_RS20970at 33.147 kb on + strand, within RR42_RS20970at 33.147 kb on + strand, within RR42_RS20970at 33.147 kb on + strand, within RR42_RS20970at 33.148 kb on - strand, within RR42_RS20970at 33.628 kb on + strand, within RR42_RS20975at 33.629 kb on - strand, within RR42_RS20975at 33.687 kb on + strand, within RR42_RS20975at 33.688 kb on - strand, within RR42_RS20975at 34.151 kb on + strand, within RR42_RS20975at 34.151 kb on + strand, within RR42_RS20975at 34.166 kb on + strand, within RR42_RS20975at 34.166 kb on + strand, within RR42_RS20975at 34.167 kb on - strand, within RR42_RS20975at 34.299 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM
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31,438 - RR42_RS20965 0.42 -0.8
31,881 - RR42_RS20965 0.80 -0.8
31,881 - RR42_RS20965 0.80 -0.9
32,122 - +0.5
32,212 - -0.8
32,292 + +0.3
32,293 - -0.4
32,556 + RR42_RS20970 0.29 +0.0
32,676 + RR42_RS20970 0.38 -0.0
32,776 + RR42_RS20970 0.46 +0.2
32,826 + RR42_RS20970 0.50 -1.3
32,826 + RR42_RS20970 0.50 -0.6
32,827 - RR42_RS20970 0.50 -1.2
32,827 - RR42_RS20970 0.50 -0.3
32,827 - RR42_RS20970 0.50 +1.5
32,871 + RR42_RS20970 0.53 -0.9
32,872 - RR42_RS20970 0.53 -1.2
33,000 + RR42_RS20970 0.63 -0.2
33,122 - RR42_RS20970 0.73 +2.7
33,122 - RR42_RS20970 0.73 -1.0
33,147 + RR42_RS20970 0.75 -0.8
33,147 + RR42_RS20970 0.75 +0.2
33,147 + RR42_RS20970 0.75 -0.4
33,148 - RR42_RS20970 0.75 -1.0
33,628 + RR42_RS20975 0.20 -0.2
33,629 - RR42_RS20975 0.20 +0.4
33,687 + RR42_RS20975 0.27 +0.6
33,688 - RR42_RS20975 0.27 +0.5
34,151 + RR42_RS20975 0.84 -0.3
34,151 + RR42_RS20975 0.84 +1.7
34,166 + RR42_RS20975 0.86 +0.2
34,166 + RR42_RS20975 0.86 -0.5
34,167 - RR42_RS20975 0.86 -1.1
34,299 - +1.5

Or see this region's nucleotide sequence