Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15725 and RR42_RS15730 are separated by 48 nucleotides RR42_RS15730 and RR42_RS15735 overlap by 1 nucleotides
RR42_RS15725: RR42_RS15725 - hypothetical protein, at 3,399,837 to 3,400,478
_RS15725
RR42_RS15730: RR42_RS15730 - sugar transferase, at 3,400,527 to 3,401,123
_RS15730
RR42_RS15735: RR42_RS15735 - pyridoxal phosphate-dependent aminotransferase, at 3,401,123 to 3,402,277
_RS15735
Position (kb)
3399
3400
3401 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3398.855 kb on + strand at 3398.855 kb on + strand at 3398.856 kb on - strand at 3398.856 kb on - strand at 3398.918 kb on - strand at 3398.991 kb on + strand at 3398.991 kb on + strand at 3398.991 kb on + strand at 3398.991 kb on + strand at 3398.992 kb on - strand at 3398.992 kb on - strand at 3398.992 kb on - strand at 3398.992 kb on - strand at 3398.992 kb on - strand at 3399.084 kb on + strand at 3399.103 kb on + strand at 3399.103 kb on + strand at 3399.104 kb on - strand at 3399.128 kb on - strand at 3399.157 kb on + strand at 3399.157 kb on + strand at 3399.190 kb on + strand at 3399.190 kb on + strand at 3399.191 kb on - strand at 3399.191 kb on - strand at 3399.238 kb on + strand at 3399.239 kb on - strand at 3399.391 kb on - strand at 3399.436 kb on + strand at 3399.437 kb on - strand at 3399.437 kb on - strand at 3399.500 kb on + strand at 3399.500 kb on + strand at 3399.501 kb on - strand at 3399.552 kb on - strand at 3399.558 kb on + strand at 3399.601 kb on + strand at 3399.670 kb on + strand at 3399.670 kb on + strand at 3399.672 kb on + strand at 3399.685 kb on - strand at 3399.707 kb on + strand at 3399.823 kb on - strand at 3399.823 kb on - strand at 3399.828 kb on + strand at 3399.990 kb on + strand, within RR42_RS15725 at 3400.052 kb on - strand, within RR42_RS15725 at 3400.071 kb on - strand, within RR42_RS15725 at 3400.095 kb on - strand, within RR42_RS15725 at 3400.095 kb on - strand, within RR42_RS15725 at 3400.095 kb on - strand, within RR42_RS15725 at 3400.225 kb on + strand, within RR42_RS15725 at 3400.226 kb on - strand, within RR42_RS15725 at 3400.559 kb on + strand at 3400.598 kb on - strand, within RR42_RS15730 at 3400.598 kb on - strand, within RR42_RS15730 at 3400.598 kb on - strand, within RR42_RS15730 at 3400.610 kb on - strand, within RR42_RS15730 at 3400.641 kb on - strand, within RR42_RS15730 at 3400.642 kb on + strand, within RR42_RS15730 at 3400.643 kb on - strand, within RR42_RS15730 at 3400.643 kb on - strand, within RR42_RS15730 at 3400.762 kb on + strand, within RR42_RS15730 at 3400.774 kb on + strand, within RR42_RS15730 at 3400.774 kb on + strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.858 kb on + strand, within RR42_RS15730 at 3400.858 kb on + strand, within RR42_RS15730 at 3400.890 kb on - strand, within RR42_RS15730 at 3400.958 kb on + strand, within RR42_RS15730 at 3400.958 kb on + strand, within RR42_RS15730 at 3400.959 kb on - strand, within RR42_RS15730 at 3401.059 kb on - strand, within RR42_RS15730 at 3401.129 kb on - strand at 3401.179 kb on + strand at 3401.187 kb on + strand at 3401.200 kb on + strand at 3401.200 kb on + strand at 3401.214 kb on + strand at 3401.271 kb on + strand, within RR42_RS15735 at 3401.296 kb on - strand, within RR42_RS15735 at 3401.296 kb on - strand, within RR42_RS15735 at 3401.306 kb on - strand, within RR42_RS15735 at 3401.385 kb on + strand, within RR42_RS15735 at 3401.385 kb on + strand, within RR42_RS15735 at 3401.405 kb on - strand, within RR42_RS15735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 3,398,855 + -0.1 3,398,855 + +0.2 3,398,856 - -1.0 3,398,856 - +2.2 3,398,918 - +0.5 3,398,991 + -0.4 3,398,991 + -0.1 3,398,991 + -0.5 3,398,991 + -1.0 3,398,992 - +0.8 3,398,992 - +0.3 3,398,992 - -0.4 3,398,992 - +0.3 3,398,992 - -0.9 3,399,084 + +1.2 3,399,103 + -0.4 3,399,103 + -0.3 3,399,104 - -0.6 3,399,128 - +1.6 3,399,157 + +0.5 3,399,157 + +1.0 3,399,190 + -0.5 3,399,190 + -0.4 3,399,191 - +1.2 3,399,191 - -0.0 3,399,238 + +0.2 3,399,239 - +0.5 3,399,391 - +0.7 3,399,436 + -0.4 3,399,437 - -0.2 3,399,437 - +0.6 3,399,500 + +0.5 3,399,500 + +0.2 3,399,501 - +0.8 3,399,552 - +1.0 3,399,558 + -0.0 3,399,601 + -0.2 3,399,670 + +1.0 3,399,670 + +0.3 3,399,672 + +1.2 3,399,685 - -0.2 3,399,707 + +1.3 3,399,823 - -0.6 3,399,823 - +0.8 3,399,828 + -0.4 3,399,990 + RR42_RS15725 0.24 +0.2 3,400,052 - RR42_RS15725 0.33 +1.6 3,400,071 - RR42_RS15725 0.36 +0.4 3,400,095 - RR42_RS15725 0.40 -0.1 3,400,095 - RR42_RS15725 0.40 -1.3 3,400,095 - RR42_RS15725 0.40 -1.0 3,400,225 + RR42_RS15725 0.60 -0.2 3,400,226 - RR42_RS15725 0.61 -1.0 3,400,559 + -2.6 3,400,598 - RR42_RS15730 0.12 +0.7 3,400,598 - RR42_RS15730 0.12 +0.2 3,400,598 - RR42_RS15730 0.12 -1.1 3,400,610 - RR42_RS15730 0.14 -0.9 3,400,641 - RR42_RS15730 0.19 +0.8 3,400,642 + RR42_RS15730 0.19 -0.8 3,400,643 - RR42_RS15730 0.19 -0.2 3,400,643 - RR42_RS15730 0.19 -1.7 3,400,762 + RR42_RS15730 0.39 -0.8 3,400,774 + RR42_RS15730 0.41 -2.6 3,400,774 + RR42_RS15730 0.41 -1.7 3,400,775 - RR42_RS15730 0.42 -2.1 3,400,775 - RR42_RS15730 0.42 -1.7 3,400,775 - RR42_RS15730 0.42 -1.0 3,400,775 - RR42_RS15730 0.42 -1.0 3,400,858 + RR42_RS15730 0.55 -1.9 3,400,858 + RR42_RS15730 0.55 +1.6 3,400,890 - RR42_RS15730 0.61 -0.7 3,400,958 + RR42_RS15730 0.72 +1.2 3,400,958 + RR42_RS15730 0.72 -2.1 3,400,959 - RR42_RS15730 0.72 -1.3 3,401,059 - RR42_RS15730 0.89 +0.3 3,401,129 - -3.1 3,401,179 + -0.8 3,401,187 + -0.2 3,401,200 + -2.2 3,401,200 + -2.3 3,401,214 + -3.2 3,401,271 + RR42_RS15735 0.13 -0.2 3,401,296 - RR42_RS15735 0.15 -0.7 3,401,296 - RR42_RS15735 0.15 -3.3 3,401,306 - RR42_RS15735 0.16 -1.6 3,401,385 + RR42_RS15735 0.23 -0.6 3,401,385 + RR42_RS15735 0.23 -2.2 3,401,405 - RR42_RS15735 0.24 +2.2
Or see this region's nucleotide sequence