Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS14020

Experiment: R2A with copper (II) chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14015 and RR42_RS14020 overlap by 4 nucleotidesRR42_RS14020 and RR42_RS14025 overlap by 4 nucleotidesRR42_RS14025 and RR42_RS14030 are separated by 30 nucleotides RR42_RS14015: RR42_RS14015 - beta-hexosaminidase, at 3,018,161 to 3,019,210 _RS14015 RR42_RS14020: RR42_RS14020 - 4'-phosphopantetheinyl transferase, at 3,019,207 to 3,019,650 _RS14020 RR42_RS14025: RR42_RS14025 - pyridoxine 5'-phosphate synthase, at 3,019,647 to 3,020,429 _RS14025 RR42_RS14030: RR42_RS14030 - DNA recombination protein RecO, at 3,020,460 to 3,021,338 _RS14030 Position (kb) 3019 3020Strain fitness (log2 ratio) -3 -2 -1 0 1at 3018.208 kb on - strandat 3018.343 kb on + strand, within RR42_RS14015at 3018.343 kb on + strand, within RR42_RS14015at 3018.343 kb on + strand, within RR42_RS14015at 3018.343 kb on + strand, within RR42_RS14015at 3018.344 kb on - strand, within RR42_RS14015at 3018.535 kb on - strand, within RR42_RS14015at 3018.535 kb on - strand, within RR42_RS14015at 3018.535 kb on - strand, within RR42_RS14015at 3018.804 kb on + strand, within RR42_RS14015at 3018.874 kb on - strand, within RR42_RS14015at 3018.874 kb on - strand, within RR42_RS14015at 3019.643 kb on + strandat 3019.644 kb on - strandat 3019.672 kb on + strandat 3020.443 kb on - strandat 3020.457 kb on - strandat 3020.519 kb on - strandat 3020.577 kb on - strand, within RR42_RS14030at 3020.578 kb on + strand, within RR42_RS14030at 3020.579 kb on - strand, within RR42_RS14030

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM
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3,018,208 - -1.1
3,018,343 + RR42_RS14015 0.17 -2.3
3,018,343 + RR42_RS14015 0.17 -0.7
3,018,343 + RR42_RS14015 0.17 -0.9
3,018,343 + RR42_RS14015 0.17 -0.1
3,018,344 - RR42_RS14015 0.17 -0.9
3,018,535 - RR42_RS14015 0.36 -0.5
3,018,535 - RR42_RS14015 0.36 -2.9
3,018,535 - RR42_RS14015 0.36 -1.5
3,018,804 + RR42_RS14015 0.61 -2.5
3,018,874 - RR42_RS14015 0.68 -2.1
3,018,874 - RR42_RS14015 0.68 -1.8
3,019,643 + -2.5
3,019,644 - +0.1
3,019,672 + -0.9
3,020,443 - +0.0
3,020,457 - -0.6
3,020,519 - +0.1
3,020,577 - RR42_RS14030 0.13 +1.0
3,020,578 + RR42_RS14030 0.13 -0.9
3,020,579 - RR42_RS14030 0.14 +0.3

Or see this region's nucleotide sequence