Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10020 and RR42_RS10025 are separated by 93 nucleotides RR42_RS10025 and RR42_RS10030 are separated by 324 nucleotides RR42_RS10030 and RR42_RS10035 are separated by 669 nucleotides
RR42_RS10020: RR42_RS10020 - 4-methylmuconolactone methylisomerase, at 2,140,869 to 2,141,207
_RS10020
RR42_RS10025: RR42_RS10025 - muconolactone delta-isomerase, at 2,141,301 to 2,141,576
_RS10025
RR42_RS10030: RR42_RS10030 - 3-hydroxyisobutyrate dehydrogenase, at 2,141,901 to 2,142,290
_RS10030
RR42_RS10035: RR42_RS10035 - 16S ribosomal RNA, at 2,142,960 to 2,144,533
_RS10035
Position (kb)
2141
2142
2143 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2140.983 kb on + strand, within RR42_RS10020 at 2140.983 kb on + strand, within RR42_RS10020 at 2141.091 kb on + strand, within RR42_RS10020 at 2141.100 kb on + strand, within RR42_RS10020 at 2141.101 kb on - strand, within RR42_RS10020 at 2141.101 kb on - strand, within RR42_RS10020 at 2141.101 kb on - strand, within RR42_RS10020 at 2141.307 kb on + strand at 2141.428 kb on + strand, within RR42_RS10025 at 2141.428 kb on + strand, within RR42_RS10025 at 2141.428 kb on + strand, within RR42_RS10025 at 2141.428 kb on + strand, within RR42_RS10025 at 2141.429 kb on - strand, within RR42_RS10025 at 2141.429 kb on - strand, within RR42_RS10025 at 2141.449 kb on - strand, within RR42_RS10025 at 2141.526 kb on + strand, within RR42_RS10025 at 2141.526 kb on + strand, within RR42_RS10025 at 2141.527 kb on - strand, within RR42_RS10025 at 2141.527 kb on - strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.528 kb on + strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.529 kb on - strand, within RR42_RS10025 at 2141.668 kb on + strand at 2141.977 kb on + strand, within RR42_RS10030 at 2141.977 kb on + strand, within RR42_RS10030 at 2141.977 kb on + strand, within RR42_RS10030 at 2141.978 kb on - strand, within RR42_RS10030 at 2141.978 kb on - strand, within RR42_RS10030 at 2142.001 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.058 kb on + strand, within RR42_RS10030 at 2142.059 kb on - strand, within RR42_RS10030 at 2142.193 kb on + strand, within RR42_RS10030 at 2142.193 kb on + strand, within RR42_RS10030 at 2142.194 kb on - strand, within RR42_RS10030 at 2142.194 kb on - strand, within RR42_RS10030 at 2142.226 kb on + strand, within RR42_RS10030 at 2142.226 kb on + strand, within RR42_RS10030 at 2142.227 kb on - strand, within RR42_RS10030 at 2142.252 kb on + strand at 2142.305 kb on + strand at 2142.306 kb on - strand at 2142.306 kb on - strand at 2142.306 kb on - strand at 2142.548 kb on + strand at 2142.549 kb on - strand at 2142.552 kb on - strand at 2142.552 kb on - strand at 2142.618 kb on - strand at 2142.633 kb on + strand at 2142.634 kb on - strand at 2142.642 kb on + strand at 2142.705 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 2,140,983 + RR42_RS10020 0.34 +1.6 2,140,983 + RR42_RS10020 0.34 -1.7 2,141,091 + RR42_RS10020 0.65 +1.4 2,141,100 + RR42_RS10020 0.68 +0.5 2,141,101 - RR42_RS10020 0.68 +0.7 2,141,101 - RR42_RS10020 0.68 +0.7 2,141,101 - RR42_RS10020 0.68 -0.7 2,141,307 + -1.0 2,141,428 + RR42_RS10025 0.46 +0.7 2,141,428 + RR42_RS10025 0.46 +0.5 2,141,428 + RR42_RS10025 0.46 +1.2 2,141,428 + RR42_RS10025 0.46 +2.7 2,141,429 - RR42_RS10025 0.46 +0.3 2,141,429 - RR42_RS10025 0.46 +0.7 2,141,449 - RR42_RS10025 0.54 -0.3 2,141,526 + RR42_RS10025 0.82 -1.2 2,141,526 + RR42_RS10025 0.82 -2.2 2,141,527 - RR42_RS10025 0.82 +0.9 2,141,527 - RR42_RS10025 0.82 -0.9 2,141,528 + RR42_RS10025 0.82 +2.3 2,141,528 + RR42_RS10025 0.82 +0.5 2,141,528 + RR42_RS10025 0.82 +0.0 2,141,528 + RR42_RS10025 0.82 +0.2 2,141,528 + RR42_RS10025 0.82 +0.1 2,141,529 - RR42_RS10025 0.83 +1.1 2,141,529 - RR42_RS10025 0.83 +0.3 2,141,529 - RR42_RS10025 0.83 +1.7 2,141,529 - RR42_RS10025 0.83 -0.1 2,141,529 - RR42_RS10025 0.83 +0.4 2,141,529 - RR42_RS10025 0.83 +0.3 2,141,668 + +1.7 2,141,977 + RR42_RS10030 0.19 +0.7 2,141,977 + RR42_RS10030 0.19 -0.1 2,141,977 + RR42_RS10030 0.19 +0.5 2,141,978 - RR42_RS10030 0.20 +0.6 2,141,978 - RR42_RS10030 0.20 +0.7 2,142,001 + RR42_RS10030 0.26 +0.2 2,142,058 + RR42_RS10030 0.40 +0.0 2,142,058 + RR42_RS10030 0.40 +0.2 2,142,058 + RR42_RS10030 0.40 -0.7 2,142,058 + RR42_RS10030 0.40 -0.6 2,142,058 + RR42_RS10030 0.40 -1.2 2,142,059 - RR42_RS10030 0.41 -1.1 2,142,193 + RR42_RS10030 0.75 +1.1 2,142,193 + RR42_RS10030 0.75 +0.1 2,142,194 - RR42_RS10030 0.75 -0.6 2,142,194 - RR42_RS10030 0.75 +0.5 2,142,226 + RR42_RS10030 0.83 -0.4 2,142,226 + RR42_RS10030 0.83 +0.5 2,142,227 - RR42_RS10030 0.84 +0.4 2,142,252 + +0.5 2,142,305 + -0.6 2,142,306 - +0.2 2,142,306 - -0.7 2,142,306 - -1.0 2,142,548 + +0.0 2,142,549 - +0.8 2,142,552 - -0.2 2,142,552 - -0.3 2,142,618 - +0.2 2,142,633 + -0.8 2,142,634 - +1.3 2,142,642 + -1.8 2,142,705 - -0.0
Or see this region's nucleotide sequence