Experiment: R2A with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02755 and RR42_RS02760 overlap by 4 nucleotides RR42_RS02760 and RR42_RS02765 are separated by 31 nucleotides RR42_RS02765 and RR42_RS02770 are separated by 156 nucleotides
RR42_RS02755: RR42_RS02755 - branched-chain amino acid transporter, at 613,186 to 613,524
_RS02755
RR42_RS02760: RR42_RS02760 - branched-chain amino acid ABC transporter permease, at 613,521 to 614,273
_RS02760
RR42_RS02765: RR42_RS02765 - aminoglycoside phosphotransferase, at 614,305 to 615,339
_RS02765
RR42_RS02770: RR42_RS02770 - LPS biosynthesis protein, at 615,496 to 617,904
_RS02770
Position (kb) 614
615
616 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 613.656 kb on + strand, within RR42_RS02760 at 613.656 kb on + strand, within RR42_RS02760 at 613.927 kb on + strand, within RR42_RS02760 at 613.927 kb on + strand, within RR42_RS02760 at 613.927 kb on + strand, within RR42_RS02760 at 613.927 kb on + strand, within RR42_RS02760 at 613.928 kb on - strand, within RR42_RS02760 at 614.161 kb on + strand, within RR42_RS02760 at 614.161 kb on + strand, within RR42_RS02760 at 614.161 kb on + strand, within RR42_RS02760 at 614.162 kb on - strand, within RR42_RS02760 at 614.315 kb on + strand at 614.315 kb on + strand at 614.360 kb on - strand at 614.360 kb on - strand at 614.381 kb on + strand at 614.406 kb on - strand at 614.459 kb on + strand, within RR42_RS02765 at 614.583 kb on - strand, within RR42_RS02765 at 614.625 kb on + strand, within RR42_RS02765 at 614.625 kb on + strand, within RR42_RS02765 at 614.626 kb on - strand, within RR42_RS02765 at 614.626 kb on - strand, within RR42_RS02765 at 614.627 kb on + strand, within RR42_RS02765 at 614.627 kb on + strand, within RR42_RS02765 at 614.627 kb on + strand, within RR42_RS02765 at 614.627 kb on + strand, within RR42_RS02765 at 614.627 kb on + strand, within RR42_RS02765 at 614.628 kb on - strand, within RR42_RS02765 at 614.628 kb on - strand, within RR42_RS02765 at 614.628 kb on - strand, within RR42_RS02765 at 614.628 kb on - strand, within RR42_RS02765 at 614.628 kb on - strand, within RR42_RS02765 at 614.657 kb on + strand, within RR42_RS02765 at 614.657 kb on + strand, within RR42_RS02765 at 614.657 kb on + strand, within RR42_RS02765 at 614.657 kb on + strand, within RR42_RS02765 at 614.657 kb on + strand, within RR42_RS02765 at 614.657 kb on + strand, within RR42_RS02765 at 614.658 kb on - strand, within RR42_RS02765 at 614.658 kb on - strand, within RR42_RS02765 at 614.658 kb on - strand, within RR42_RS02765 at 614.658 kb on - strand, within RR42_RS02765 at 614.658 kb on - strand, within RR42_RS02765 at 614.658 kb on - strand, within RR42_RS02765 at 614.661 kb on + strand, within RR42_RS02765 at 614.662 kb on - strand, within RR42_RS02765 at 614.662 kb on - strand, within RR42_RS02765 at 614.745 kb on - strand, within RR42_RS02765 at 614.759 kb on + strand, within RR42_RS02765 at 614.759 kb on + strand, within RR42_RS02765 at 614.759 kb on + strand, within RR42_RS02765 at 614.759 kb on + strand, within RR42_RS02765 at 614.759 kb on + strand, within RR42_RS02765 at 614.759 kb on + strand, within RR42_RS02765 at 614.759 kb on + strand, within RR42_RS02765 at 614.760 kb on - strand, within RR42_RS02765 at 614.760 kb on - strand, within RR42_RS02765 at 614.760 kb on - strand, within RR42_RS02765 at 614.760 kb on - strand, within RR42_RS02765 at 614.760 kb on - strand, within RR42_RS02765 at 614.760 kb on - strand, within RR42_RS02765 at 614.864 kb on + strand, within RR42_RS02765 at 614.864 kb on + strand, within RR42_RS02765 at 614.865 kb on - strand, within RR42_RS02765 at 614.978 kb on + strand, within RR42_RS02765 at 615.017 kb on + strand, within RR42_RS02765 at 615.017 kb on + strand, within RR42_RS02765 at 615.017 kb on + strand, within RR42_RS02765 at 615.017 kb on + strand, within RR42_RS02765 at 615.017 kb on + strand, within RR42_RS02765 at 615.018 kb on - strand, within RR42_RS02765 at 615.018 kb on - strand, within RR42_RS02765 at 615.018 kb on - strand, within RR42_RS02765 at 615.018 kb on - strand, within RR42_RS02765 at 615.018 kb on - strand, within RR42_RS02765 at 615.018 kb on - strand, within RR42_RS02765 at 615.018 kb on - strand, within RR42_RS02765 at 615.018 kb on - strand, within RR42_RS02765 at 615.018 kb on - strand, within RR42_RS02765 at 615.355 kb on + strand at 615.355 kb on + strand at 615.356 kb on - strand at 615.356 kb on - strand at 615.356 kb on - strand at 615.368 kb on - strand at 615.368 kb on - strand at 615.368 kb on - strand at 615.368 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.25 mM remove 613,656 + RR42_RS02760 0.18 -0.4 613,656 + RR42_RS02760 0.18 -0.8 613,927 + RR42_RS02760 0.54 -1.0 613,927 + RR42_RS02760 0.54 -1.8 613,927 + RR42_RS02760 0.54 -1.3 613,927 + RR42_RS02760 0.54 -2.7 613,928 - RR42_RS02760 0.54 -0.1 614,161 + RR42_RS02760 0.85 -1.7 614,161 + RR42_RS02760 0.85 +0.8 614,161 + RR42_RS02760 0.85 +0.2 614,162 - RR42_RS02760 0.85 +1.5 614,315 + -2.6 614,315 + -0.6 614,360 - +0.2 614,360 - -2.5 614,381 + -1.4 614,406 - -2.0 614,459 + RR42_RS02765 0.15 -1.9 614,583 - RR42_RS02765 0.27 -0.4 614,625 + RR42_RS02765 0.31 -1.7 614,625 + RR42_RS02765 0.31 +0.2 614,626 - RR42_RS02765 0.31 +0.2 614,626 - RR42_RS02765 0.31 -1.4 614,627 + RR42_RS02765 0.31 -2.6 614,627 + RR42_RS02765 0.31 -1.2 614,627 + RR42_RS02765 0.31 -1.5 614,627 + RR42_RS02765 0.31 -1.4 614,627 + RR42_RS02765 0.31 -1.1 614,628 - RR42_RS02765 0.31 -1.0 614,628 - RR42_RS02765 0.31 -1.5 614,628 - RR42_RS02765 0.31 -1.0 614,628 - RR42_RS02765 0.31 -0.1 614,628 - RR42_RS02765 0.31 +0.6 614,657 + RR42_RS02765 0.34 -1.7 614,657 + RR42_RS02765 0.34 -0.1 614,657 + RR42_RS02765 0.34 -2.9 614,657 + RR42_RS02765 0.34 -1.4 614,657 + RR42_RS02765 0.34 -1.3 614,657 + RR42_RS02765 0.34 +0.3 614,658 - RR42_RS02765 0.34 +0.2 614,658 - RR42_RS02765 0.34 -0.5 614,658 - RR42_RS02765 0.34 -2.6 614,658 - RR42_RS02765 0.34 -1.7 614,658 - RR42_RS02765 0.34 +1.2 614,658 - RR42_RS02765 0.34 +0.3 614,661 + RR42_RS02765 0.34 -0.4 614,662 - RR42_RS02765 0.34 -1.6 614,662 - RR42_RS02765 0.34 -4.2 614,745 - RR42_RS02765 0.43 -1.5 614,759 + RR42_RS02765 0.44 -1.4 614,759 + RR42_RS02765 0.44 +0.6 614,759 + RR42_RS02765 0.44 -1.3 614,759 + RR42_RS02765 0.44 -1.2 614,759 + RR42_RS02765 0.44 -0.9 614,759 + RR42_RS02765 0.44 -1.8 614,759 + RR42_RS02765 0.44 +0.5 614,760 - RR42_RS02765 0.44 -0.8 614,760 - RR42_RS02765 0.44 -0.9 614,760 - RR42_RS02765 0.44 -1.0 614,760 - RR42_RS02765 0.44 +1.2 614,760 - RR42_RS02765 0.44 -3.2 614,760 - RR42_RS02765 0.44 -1.5 614,864 + RR42_RS02765 0.54 -2.0 614,864 + RR42_RS02765 0.54 -0.1 614,865 - RR42_RS02765 0.54 -1.6 614,978 + RR42_RS02765 0.65 +1.4 615,017 + RR42_RS02765 0.69 -2.9 615,017 + RR42_RS02765 0.69 -0.4 615,017 + RR42_RS02765 0.69 -1.0 615,017 + RR42_RS02765 0.69 -0.6 615,017 + RR42_RS02765 0.69 -1.3 615,018 - RR42_RS02765 0.69 +1.5 615,018 - RR42_RS02765 0.69 -1.8 615,018 - RR42_RS02765 0.69 +0.5 615,018 - RR42_RS02765 0.69 -1.1 615,018 - RR42_RS02765 0.69 -1.2 615,018 - RR42_RS02765 0.69 +0.3 615,018 - RR42_RS02765 0.69 -1.7 615,018 - RR42_RS02765 0.69 -1.5 615,018 - RR42_RS02765 0.69 -1.3 615,355 + -0.5 615,355 + +2.2 615,356 - +2.0 615,356 - +0.2 615,356 - -0.1 615,368 - -1.2 615,368 - -0.4 615,368 - -0.9 615,368 - +0.4
Or see this region's nucleotide sequence