Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS13950

Experiment: Temperature shift 30_to_25

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Temperature shift 30_to_25
3,134,044	+				-0.7
3,134,044	+				+3.4
3,134,062	+				-2.8
3,134,063	-				-2.8
3,134,063	-				-0.5
3,134,127	-		PFLU_RS13940	0.21	+0.6
3,134,169	+		PFLU_RS13940	0.34	+2.0
3,134,180	+		PFLU_RS13940	0.37	+0.6
3,134,200	+		PFLU_RS13940	0.43	+1.2
3,134,200	+		PFLU_RS13940	0.43	-0.2
3,134,200	+		PFLU_RS13940	0.43	-1.0
3,134,200	+		PFLU_RS13940	0.43	+0.3
3,134,200	+		PFLU_RS13940	0.43	+0.3
3,134,200	+		PFLU_RS13940	0.43	-0.9
3,134,200	+		PFLU_RS13940	0.43	-3.2
3,134,200	+		PFLU_RS13940	0.43	+0.2
3,134,200	+		PFLU_RS13940	0.43	+1.0
3,134,201	-		PFLU_RS13940	0.43	+0.6
3,134,201	-		PFLU_RS13940	0.43	-1.7
3,134,201	-		PFLU_RS13940	0.43	+0.6
3,134,201	-		PFLU_RS13940	0.43	+0.7
3,134,201	-		PFLU_RS13940	0.43	+1.2
3,134,201	-		PFLU_RS13940	0.43	+2.1
3,134,235	-		PFLU_RS13940	0.54	+2.5
3,134,245	+		PFLU_RS13940	0.57	-0.9
3,134,259	-		PFLU_RS13940	0.61	-3.1
3,134,259	-		PFLU_RS13940	0.61	+2.0
3,134,309	-		PFLU_RS13940	0.76	+1.2
3,134,309	-		PFLU_RS13940	0.76	+0.2
3,134,309	-		PFLU_RS13940	0.76	-1.4
3,134,310	+		PFLU_RS13940	0.76	+0.9
3,134,310	+		PFLU_RS13940	0.76	+1.6
3,134,310	+		PFLU_RS13940	0.76	+2.6
3,134,310	+		PFLU_RS13940	0.76	+1.2
3,134,310	+		PFLU_RS13940	0.76	+0.2
3,134,310	+		PFLU_RS13940	0.76	+1.0
3,134,310	+		PFLU_RS13940	0.76	+1.3
3,134,310	+		PFLU_RS13940	0.76	-0.6
3,134,310	+		PFLU_RS13940	0.76	-1.1
3,134,310	+		PFLU_RS13940	0.76	+1.0
3,134,310	+		PFLU_RS13940	0.76	+0.6
3,134,310	+		PFLU_RS13940	0.76	+2.0
3,134,310	+		PFLU_RS13940	0.76	-0.5
3,134,310	+		PFLU_RS13940	0.76	+1.0
3,134,310	+		PFLU_RS13940	0.76	-3.0
3,134,310	+		PFLU_RS13940	0.76	+0.4
3,134,310	+		PFLU_RS13940	0.76	-0.4
3,134,310	+		PFLU_RS13940	0.76	-3.0
3,134,311	-		PFLU_RS13940	0.76	+1.0
3,134,311	-		PFLU_RS13940	0.76	-1.0
3,134,311	-		PFLU_RS13940	0.76	+0.6
3,134,311	-		PFLU_RS13940	0.76	-1.2
3,134,311	-		PFLU_RS13940	0.76	-0.7
3,134,311	-		PFLU_RS13940	0.76	-0.7
3,134,311	-		PFLU_RS13940	0.76	+2.1
3,134,311	-		PFLU_RS13940	0.76	-0.4
3,134,311	-		PFLU_RS13940	0.76	+1.6
3,134,311	-		PFLU_RS13940	0.76	+1.6
3,134,311	-		PFLU_RS13940	0.76	+0.5
3,134,311	-		PFLU_RS13940	0.76	-0.7
3,134,312	+		PFLU_RS13940	0.76	+0.2
3,134,312	+		PFLU_RS13940	0.76	-0.5
3,134,312	+		PFLU_RS13940	0.76	+0.4
3,134,312	+		PFLU_RS13940	0.76	-0.1
3,134,312	+		PFLU_RS13940	0.76	+1.4
3,134,312	+		PFLU_RS13940	0.76	-0.7
3,134,312	+		PFLU_RS13940	0.76	+1.4
3,134,312	+		PFLU_RS13940	0.76	+0.6
3,134,312	+		PFLU_RS13940	0.76	+1.5
3,134,312	+		PFLU_RS13940	0.76	+1.1
3,134,312	+		PFLU_RS13940	0.76	+0.1
3,134,312	+		PFLU_RS13940	0.76	-0.4
3,134,312	+		PFLU_RS13940	0.76	-1.5
3,134,312	+		PFLU_RS13940	0.76	+2.1
3,134,312	+		PFLU_RS13940	0.76	-1.7
3,134,312	+		PFLU_RS13940	0.76	-0.7
3,134,313	-		PFLU_RS13940	0.77	-2.2
3,134,313	-		PFLU_RS13940	0.77	-0.4
3,134,313	-		PFLU_RS13940	0.77	-2.0
3,134,313	-		PFLU_RS13940	0.77	-1.4
3,134,313	-		PFLU_RS13940	0.77	-2.4
3,134,313	-		PFLU_RS13940	0.77	-0.4
3,134,313	-		PFLU_RS13940	0.77	+1.0
3,134,313	-		PFLU_RS13940	0.77	-1.7
3,134,313	-		PFLU_RS13940	0.77	-1.4
3,134,313	-		PFLU_RS13940	0.77	-1.7
3,134,313	-		PFLU_RS13940	0.77	-1.4
3,134,313	-		PFLU_RS13940	0.77	-2.4
3,134,313	-		PFLU_RS13940	0.77	-1.4
3,134,313	-		PFLU_RS13940	0.77	-0.5
3,134,313	-		PFLU_RS13940	0.77	+1.1
3,134,332	-		PFLU_RS13940	0.82	+1.0
3,134,346	+		PFLU_RS13940	0.87	+0.8
3,134,346	+		PFLU_RS13940	0.87	+0.4
3,134,346	+		PFLU_RS13940	0.87	+0.4
3,134,346	+		PFLU_RS13940	0.87	-2.1
3,134,347	-		PFLU_RS13940	0.87	+1.1
3,134,347	-		PFLU_RS13940	0.87	+0.5
3,134,347	-		PFLU_RS13940	0.87	+0.0
3,134,347	-		PFLU_RS13940	0.87	+1.1
3,134,348	+		PFLU_RS13940	0.87	+0.0
3,134,349	-		PFLU_RS13940	0.88	+1.0
3,134,349	-		PFLU_RS13940	0.88	-0.8
3,134,361	+				+0.4
3,134,417	+				+0.8
3,134,417	+				-0.4
3,134,417	+				-0.4
3,134,417	+				-0.4
3,134,418	-				+2.6
3,134,418	-				+0.5
3,134,418	-				-2.4
3,134,418	-				+0.1
3,134,418	-				-1.4
3,134,418	-				+0.4
3,134,418	-				-0.0
3,134,418	-				-2.0
3,134,451	+				-1.1
3,134,464	+				+1.0
3,134,464	+				+0.6
3,134,464	+				+1.2
3,134,465	-				-0.4
3,134,465	-				-0.4
3,134,465	-				-2.4
3,134,465	-				+3.3
3,134,554	+				+1.2
3,134,554	+				-0.4
3,134,554	+				-1.7
3,134,554	+				+0.5
3,134,555	-				+0.4
3,134,555	-				-1.7
3,134,555	-				+0.8
3,134,555	-				+0.7
3,134,555	-				-0.5
3,134,555	-				-4.1
3,134,555	-				+2.1
3,134,560	+				-0.2
3,134,560	+				+0.5
3,134,560	+				+0.4
3,134,560	+				-1.4
3,134,561	-				+0.5
3,134,569	+				+0.4
3,134,570	-				+2.1
3,134,570	-				+0.2
3,134,588	+				+2.0
3,134,588	+				+1.4
3,134,608	+				-0.4
3,134,608	+				-1.5
3,134,902	+		PFLU_RS13945	0.81	+2.5
3,134,903	-		PFLU_RS13945	0.81	+0.5
3,134,903	-		PFLU_RS13945	0.81	+1.3
3,134,903	-		PFLU_RS13945	0.81	-1.0
3,135,139	+		PFLU_RS13950	0.50	-3.2
3,135,139	+		PFLU_RS13950	0.50	-1.5
3,135,140	-		PFLU_RS13950	0.50	-0.4
3,135,140	-		PFLU_RS13950	0.50	-1.3
3,135,140	-		PFLU_RS13950	0.50	+0.2
3,135,450	+		PFLU_RS13955	0.40	-0.4
3,135,450	+		PFLU_RS13955	0.40	+1.3
3,135,450	+		PFLU_RS13955	0.40	+2.2
3,135,450	+		PFLU_RS13955	0.40	-1.0
3,135,450	+		PFLU_RS13955	0.40	+0.6
3,135,450	+		PFLU_RS13955	0.40	+1.0
3,135,450	+		PFLU_RS13955	0.40	+0.1
3,135,450	+		PFLU_RS13955	0.40	+0.5
3,135,450	+		PFLU_RS13955	0.40	-1.3
3,135,450	+		PFLU_RS13955	0.40	+1.1
3,135,450	+		PFLU_RS13955	0.40	+0.3
3,135,450	+		PFLU_RS13955	0.40	+0.2
3,135,450	+		PFLU_RS13955	0.40	+1.8
3,135,451	-		PFLU_RS13955	0.41	+1.3
3,135,451	-		PFLU_RS13955	0.41	-2.7
3,135,451	-		PFLU_RS13955	0.41	-1.7
3,135,451	-		PFLU_RS13955	0.41	-0.4
3,135,451	-		PFLU_RS13955	0.41	-0.6
3,135,451	-		PFLU_RS13955	0.41	+1.3
3,135,451	-		PFLU_RS13955	0.41	+0.6
3,135,451	-		PFLU_RS13955	0.41	+0.0
3,135,507	+		PFLU_RS13955	0.67	+1.2
3,135,507	+		PFLU_RS13955	0.67	+3.2
3,135,507	+		PFLU_RS13955	0.67	-0.2
3,135,507	+		PFLU_RS13955	0.67	-0.0
3,135,508	-		PFLU_RS13955	0.67	-2.0
3,135,508	-		PFLU_RS13955	0.67	-1.0
3,135,636	+		PFLU_RS13960	0.32	+0.3
3,135,636	+		PFLU_RS13960	0.32	-1.0
3,135,930	+		PFLU_RS13965	0.65	+1.8
3,135,931	-		PFLU_RS13965	0.66	+0.1
3,135,931	-		PFLU_RS13965	0.66	-0.6
3,135,960	+		PFLU_RS13965	0.77	+0.5
3,135,960	+		PFLU_RS13965	0.77	+0.7
3,135,960	+		PFLU_RS13965	0.77	+1.5
3,135,961	-		PFLU_RS13965	0.78	-1.0
3,135,961	-		PFLU_RS13965	0.78	+0.6
3,135,961	-		PFLU_RS13965	0.78	+0.1
3,136,015	+				-1.0
3,136,015	+				+0.2
3,136,015	+				-3.5
3,136,016	-				-0.2
3,136,016	-				+2.0
3,136,016	-				+0.4
3,136,016	-				+1.2
3,136,016	-				-2.4
3,136,058	+				+0.8
3,136,058	+				+1.9
3,136,059	-				+0.5
3,136,059	-				-1.8
3,136,059	-				+0.1
3,136,059	-				-0.2
3,136,059	-				+1.0
3,136,059	-				+0.6
3,136,064	+				-1.4
3,136,064	+				+1.2
3,136,064	+				-0.6
3,136,064	+				+2.4
3,136,065	-				+0.5
3,136,065	-				-0.6
3,136,065	-				+1.4
3,136,065	-				+2.2
3,136,065	-				-1.7
3,136,065	-				+2.7
3,136,065	-				-0.1
3,136,065	-				-1.1
3,136,065	-				+0.0
3,136,065	-				-0.0
3,136,140	-		PFLU_RS13970	0.32	-3.1
3,136,158	+		PFLU_RS13970	0.41	-0.4
3,136,158	+		PFLU_RS13970	0.41	+2.6
3,136,158	+		PFLU_RS13970	0.41	-0.4
3,136,158	+		PFLU_RS13970	0.41	+1.2
3,136,159	-		PFLU_RS13970	0.41	+1.0
3,136,159	-		PFLU_RS13970	0.41	+0.2
3,136,159	-		PFLU_RS13970	0.41	-0.0
3,136,159	-		PFLU_RS13970	0.41	+2.5
3,136,159	-		PFLU_RS13970	0.41	+0.2
3,136,159	-		PFLU_RS13970	0.41	-3.1
3,136,159	-		PFLU_RS13970	0.41	-0.3
3,136,223	+		PFLU_RS13970	0.70	+0.9
3,136,235	+		PFLU_RS13970	0.75	+0.6
3,136,235	+		PFLU_RS13970	0.75	-3.2
3,136,235	+		PFLU_RS13970	0.75	+1.8
3,136,235	+		PFLU_RS13970	0.75	-0.3
3,136,235	+		PFLU_RS13970	0.75	-0.4
3,136,235	+		PFLU_RS13970	0.75	+0.6
3,136,235	+		PFLU_RS13970	0.75	-0.6
3,136,235	+		PFLU_RS13970	0.75	+0.4
3,136,235	+		PFLU_RS13970	0.75	+2.0
3,136,235	+		PFLU_RS13970	0.75	+1.8
3,136,235	+		PFLU_RS13970	0.75	-0.4
3,136,235	+		PFLU_RS13970	0.75	+1.0
3,136,235	+		PFLU_RS13970	0.75	+1.4
3,136,235	+		PFLU_RS13970	0.75	+0.6
3,136,235	+		PFLU_RS13970	0.75	-0.4
3,136,235	+		PFLU_RS13970	0.75	+1.1
3,136,235	+		PFLU_RS13970	0.75	+1.4
3,136,235	+		PFLU_RS13970	0.75	+2.3
3,136,235	+		PFLU_RS13970	0.75	-0.8
3,136,235	+		PFLU_RS13970	0.75	+0.0
3,136,235	+		PFLU_RS13970	0.75	-2.5
3,136,235	+		PFLU_RS13970	0.75	-2.2
3,136,235	+		PFLU_RS13970	0.75	-1.4
3,136,235	+		PFLU_RS13970	0.75	+1.0
3,136,235	+		PFLU_RS13970	0.75	+0.4
3,136,236	-		PFLU_RS13970	0.76	+1.2
3,136,236	-		PFLU_RS13970	0.76	+2.6
3,136,236	-		PFLU_RS13970	0.76	-2.4
3,136,236	-		PFLU_RS13970	0.76	+0.6
3,136,236	-		PFLU_RS13970	0.76	-0.2
3,136,236	-		PFLU_RS13970	0.76	+1.6
3,136,236	-		PFLU_RS13970	0.76	-1.4
3,136,236	-		PFLU_RS13970	0.76	-0.7
3,136,236	-		PFLU_RS13970	0.76	+0.6
3,136,236	-		PFLU_RS13970	0.76	-0.2
3,136,236	-		PFLU_RS13970	0.76	+0.5
3,136,236	-		PFLU_RS13970	0.76	+0.1
3,136,236	-		PFLU_RS13970	0.76	+0.5
3,136,236	-		PFLU_RS13970	0.76	-2.0
3,136,236	-		PFLU_RS13970	0.76	-0.7
3,136,236	-		PFLU_RS13970	0.76	-0.1
3,136,236	-		PFLU_RS13970	0.76	-0.4
3,136,236	-		PFLU_RS13970	0.76	-2.7
3,136,236	-		PFLU_RS13970	0.76	+0.9
3,136,236	-		PFLU_RS13970	0.76	+0.4
3,136,237	+		PFLU_RS13970	0.76	+0.5
3,136,237	+		PFLU_RS13970	0.76	+0.9
3,136,237	+		PFLU_RS13970	0.76	-2.4
3,136,238	-		PFLU_RS13970	0.77	-0.5
3,136,273	+				-2.7
3,136,274	-				+0.6