Experiment: R2A with Cobalt chloride 0.08 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21555 and CA265_RS21560 are separated by 69 nucleotides CA265_RS21560 and CA265_RS21565 are separated by 104 nucleotides CA265_RS21565 and CA265_RS21570 are separated by 197 nucleotides CA265_RS21570 and CA265_RS21575 overlap by 4 nucleotides
CA265_RS21555: CA265_RS21555 - hypothetical protein, at 5,308,726 to 5,309,601
_RS21555
CA265_RS21560: CA265_RS21560 - hypothetical protein, at 5,309,671 to 5,310,597
_RS21560
CA265_RS21565: CA265_RS21565 - cold-shock protein, at 5,310,702 to 5,310,893
_RS21565
CA265_RS21570: CA265_RS21570 - hypothetical protein, at 5,311,091 to 5,311,573
_RS21570
CA265_RS21575: CA265_RS21575 - RNA polymerase subunit sigma, at 5,311,570 to 5,312,151
_RS21575
Position (kb) 5309
5310
5311 Strain fitness (log2 ratio) -1
0
1 at 5308.772 kb on + strand at 5308.773 kb on - strand at 5308.930 kb on - strand, within CA265_RS21555 at 5308.946 kb on + strand, within CA265_RS21555 at 5309.097 kb on + strand, within CA265_RS21555 at 5309.216 kb on + strand, within CA265_RS21555 at 5309.259 kb on - strand, within CA265_RS21555 at 5309.280 kb on - strand, within CA265_RS21555 at 5309.330 kb on + strand, within CA265_RS21555 at 5309.470 kb on - strand, within CA265_RS21555 at 5309.516 kb on - strand at 5309.561 kb on + strand at 5309.566 kb on - strand at 5309.612 kb on - strand at 5309.627 kb on - strand at 5309.682 kb on + strand at 5309.683 kb on - strand at 5309.781 kb on - strand, within CA265_RS21560 at 5309.893 kb on - strand, within CA265_RS21560 at 5309.893 kb on - strand, within CA265_RS21560 at 5309.893 kb on - strand, within CA265_RS21560 at 5309.940 kb on + strand, within CA265_RS21560 at 5309.941 kb on - strand, within CA265_RS21560 at 5310.066 kb on - strand, within CA265_RS21560 at 5310.078 kb on - strand, within CA265_RS21560 at 5310.104 kb on - strand, within CA265_RS21560 at 5310.186 kb on + strand, within CA265_RS21560 at 5310.186 kb on + strand, within CA265_RS21560 at 5310.189 kb on + strand, within CA265_RS21560 at 5310.190 kb on - strand, within CA265_RS21560 at 5310.243 kb on + strand, within CA265_RS21560 at 5310.363 kb on + strand, within CA265_RS21560 at 5310.364 kb on - strand, within CA265_RS21560 at 5310.376 kb on + strand, within CA265_RS21560 at 5310.413 kb on + strand, within CA265_RS21560 at 5310.413 kb on + strand, within CA265_RS21560 at 5310.413 kb on + strand, within CA265_RS21560 at 5310.414 kb on - strand, within CA265_RS21560 at 5310.414 kb on - strand, within CA265_RS21560 at 5310.420 kb on - strand, within CA265_RS21560 at 5310.430 kb on + strand, within CA265_RS21560 at 5310.450 kb on + strand, within CA265_RS21560 at 5310.599 kb on - strand at 5310.752 kb on + strand, within CA265_RS21565 at 5310.886 kb on + strand at 5310.897 kb on - strand at 5310.897 kb on - strand at 5310.901 kb on - strand at 5310.905 kb on + strand at 5310.954 kb on - strand at 5311.043 kb on + strand at 5311.044 kb on - strand at 5311.045 kb on + strand at 5311.045 kb on + strand at 5311.045 kb on + strand at 5311.181 kb on - strand, within CA265_RS21570 at 5311.256 kb on - strand, within CA265_RS21570 at 5311.257 kb on + strand, within CA265_RS21570 at 5311.319 kb on - strand, within CA265_RS21570 at 5311.372 kb on + strand, within CA265_RS21570 at 5311.576 kb on + strand at 5311.577 kb on - strand at 5311.585 kb on + strand at 5311.597 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.08 mM remove 5,308,772 + +0.4 5,308,773 - -0.3 5,308,930 - CA265_RS21555 0.23 -0.4 5,308,946 + CA265_RS21555 0.25 +0.4 5,309,097 + CA265_RS21555 0.42 -0.4 5,309,216 + CA265_RS21555 0.56 +0.3 5,309,259 - CA265_RS21555 0.61 +0.3 5,309,280 - CA265_RS21555 0.63 +0.5 5,309,330 + CA265_RS21555 0.69 +0.6 5,309,470 - CA265_RS21555 0.85 +0.4 5,309,516 - -0.5 5,309,561 + +0.7 5,309,566 - +0.0 5,309,612 - -0.2 5,309,627 - -0.4 5,309,682 + +0.0 5,309,683 - +1.1 5,309,781 - CA265_RS21560 0.12 -0.4 5,309,893 - CA265_RS21560 0.24 -0.3 5,309,893 - CA265_RS21560 0.24 -0.9 5,309,893 - CA265_RS21560 0.24 +0.0 5,309,940 + CA265_RS21560 0.29 -1.2 5,309,941 - CA265_RS21560 0.29 -1.4 5,310,066 - CA265_RS21560 0.43 -0.0 5,310,078 - CA265_RS21560 0.44 +0.7 5,310,104 - CA265_RS21560 0.47 +0.7 5,310,186 + CA265_RS21560 0.56 -0.0 5,310,186 + CA265_RS21560 0.56 +1.0 5,310,189 + CA265_RS21560 0.56 -0.1 5,310,190 - CA265_RS21560 0.56 -0.0 5,310,243 + CA265_RS21560 0.62 -0.3 5,310,363 + CA265_RS21560 0.75 +0.5 5,310,364 - CA265_RS21560 0.75 -0.8 5,310,376 + CA265_RS21560 0.76 -0.5 5,310,413 + CA265_RS21560 0.80 -1.7 5,310,413 + CA265_RS21560 0.80 +0.9 5,310,413 + CA265_RS21560 0.80 -0.5 5,310,414 - CA265_RS21560 0.80 +1.5 5,310,414 - CA265_RS21560 0.80 +0.2 5,310,420 - CA265_RS21560 0.81 -0.6 5,310,430 + CA265_RS21560 0.82 +0.4 5,310,450 + CA265_RS21560 0.84 -1.0 5,310,599 - +1.0 5,310,752 + CA265_RS21565 0.26 -0.1 5,310,886 + -0.7 5,310,897 - -0.3 5,310,897 - +0.7 5,310,901 - -0.4 5,310,905 + -0.1 5,310,954 - -0.8 5,311,043 + +0.2 5,311,044 - +0.4 5,311,045 + +0.4 5,311,045 + +1.5 5,311,045 + -0.3 5,311,181 - CA265_RS21570 0.19 -1.0 5,311,256 - CA265_RS21570 0.34 -1.0 5,311,257 + CA265_RS21570 0.34 +0.1 5,311,319 - CA265_RS21570 0.47 -1.3 5,311,372 + CA265_RS21570 0.58 +1.4 5,311,576 + -0.3 5,311,577 - -0.7 5,311,585 + +0.5 5,311,597 + -0.0
Or see this region's nucleotide sequence