Experiment: R2A with Cobalt chloride 0.08 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08075 and CA265_RS08080 are separated by 8 nucleotides CA265_RS08080 and CA265_RS08085 overlap by 1 nucleotides
CA265_RS08075: CA265_RS08075 - ribonuclease BN, at 2,008,022 to 2,009,011
_RS08075
CA265_RS08080: CA265_RS08080 - thioesterase, at 2,009,020 to 2,009,427
_RS08080
CA265_RS08085: CA265_RS08085 - aminodeoxychorismate lyase, at 2,009,427 to 2,010,482
_RS08085
Position (kb)
2009
2010 Strain fitness (log2 ratio)
-2
-1
0
1 at 2008.036 kb on + strand at 2008.079 kb on - strand at 2008.127 kb on + strand, within CA265_RS08075 at 2008.206 kb on + strand, within CA265_RS08075 at 2008.218 kb on + strand, within CA265_RS08075 at 2008.237 kb on + strand, within CA265_RS08075 at 2008.240 kb on - strand, within CA265_RS08075 at 2008.277 kb on - strand, within CA265_RS08075 at 2008.322 kb on - strand, within CA265_RS08075 at 2008.365 kb on + strand, within CA265_RS08075 at 2008.366 kb on - strand, within CA265_RS08075 at 2008.379 kb on + strand, within CA265_RS08075 at 2008.380 kb on - strand, within CA265_RS08075 at 2008.521 kb on - strand, within CA265_RS08075 at 2008.535 kb on + strand, within CA265_RS08075 at 2008.787 kb on + strand, within CA265_RS08075 at 2008.901 kb on + strand, within CA265_RS08075 at 2008.902 kb on - strand, within CA265_RS08075 at 2008.902 kb on - strand, within CA265_RS08075 at 2008.908 kb on + strand, within CA265_RS08075 at 2008.967 kb on - strand at 2008.971 kb on + strand at 2008.978 kb on + strand at 2008.979 kb on - strand at 2008.989 kb on - strand at 2009.001 kb on - strand at 2009.118 kb on + strand, within CA265_RS08080 at 2009.145 kb on - strand, within CA265_RS08080 at 2009.154 kb on - strand, within CA265_RS08080 at 2009.235 kb on + strand, within CA265_RS08080 at 2009.241 kb on + strand, within CA265_RS08080 at 2009.294 kb on + strand, within CA265_RS08080 at 2009.294 kb on + strand, within CA265_RS08080 at 2009.343 kb on - strand, within CA265_RS08080 at 2009.364 kb on - strand, within CA265_RS08080 at 2009.364 kb on - strand, within CA265_RS08080 at 2009.364 kb on - strand, within CA265_RS08080 at 2009.368 kb on - strand, within CA265_RS08080 at 2009.368 kb on - strand, within CA265_RS08080 at 2009.368 kb on - strand, within CA265_RS08080 at 2009.368 kb on - strand, within CA265_RS08080 at 2009.413 kb on - strand at 2009.422 kb on - strand at 2009.429 kb on - strand at 2009.433 kb on - strand at 2009.436 kb on + strand at 2009.545 kb on + strand, within CA265_RS08085 at 2009.549 kb on + strand, within CA265_RS08085 at 2009.550 kb on - strand, within CA265_RS08085 at 2009.552 kb on - strand, within CA265_RS08085 at 2009.595 kb on - strand, within CA265_RS08085 at 2009.636 kb on + strand, within CA265_RS08085 at 2009.636 kb on + strand, within CA265_RS08085 at 2009.636 kb on + strand, within CA265_RS08085 at 2009.637 kb on - strand, within CA265_RS08085 at 2009.637 kb on - strand, within CA265_RS08085 at 2009.638 kb on + strand, within CA265_RS08085 at 2009.648 kb on - strand, within CA265_RS08085 at 2009.648 kb on - strand, within CA265_RS08085 at 2009.659 kb on - strand, within CA265_RS08085 at 2009.733 kb on + strand, within CA265_RS08085 at 2009.775 kb on + strand, within CA265_RS08085 at 2009.836 kb on - strand, within CA265_RS08085 at 2009.836 kb on - strand, within CA265_RS08085 at 2009.836 kb on - strand, within CA265_RS08085 at 2009.841 kb on + strand, within CA265_RS08085 at 2009.857 kb on - strand, within CA265_RS08085 at 2009.898 kb on - strand, within CA265_RS08085 at 2009.903 kb on - strand, within CA265_RS08085 at 2009.903 kb on - strand, within CA265_RS08085 at 2009.999 kb on + strand, within CA265_RS08085 at 2010.156 kb on + strand, within CA265_RS08085 at 2010.161 kb on + strand, within CA265_RS08085 at 2010.166 kb on - strand, within CA265_RS08085 at 2010.334 kb on - strand, within CA265_RS08085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.08 mM remove 2,008,036 + -0.4 2,008,079 - +0.1 2,008,127 + CA265_RS08075 0.11 -0.7 2,008,206 + CA265_RS08075 0.19 -1.5 2,008,218 + CA265_RS08075 0.20 +0.1 2,008,237 + CA265_RS08075 0.22 -0.0 2,008,240 - CA265_RS08075 0.22 -0.2 2,008,277 - CA265_RS08075 0.26 -0.0 2,008,322 - CA265_RS08075 0.30 +0.3 2,008,365 + CA265_RS08075 0.35 -0.6 2,008,366 - CA265_RS08075 0.35 -0.5 2,008,379 + CA265_RS08075 0.36 -1.3 2,008,380 - CA265_RS08075 0.36 -0.4 2,008,521 - CA265_RS08075 0.50 -0.2 2,008,535 + CA265_RS08075 0.52 -0.6 2,008,787 + CA265_RS08075 0.77 +0.0 2,008,901 + CA265_RS08075 0.89 +0.1 2,008,902 - CA265_RS08075 0.89 -0.6 2,008,902 - CA265_RS08075 0.89 -0.1 2,008,908 + CA265_RS08075 0.89 +0.1 2,008,967 - +0.2 2,008,971 + -1.1 2,008,978 + -0.5 2,008,979 - -0.8 2,008,989 - -0.5 2,009,001 - -0.9 2,009,118 + CA265_RS08080 0.24 -0.2 2,009,145 - CA265_RS08080 0.31 +0.7 2,009,154 - CA265_RS08080 0.33 -0.0 2,009,235 + CA265_RS08080 0.53 -1.7 2,009,241 + CA265_RS08080 0.54 -0.1 2,009,294 + CA265_RS08080 0.67 +1.7 2,009,294 + CA265_RS08080 0.67 -0.2 2,009,343 - CA265_RS08080 0.79 -0.4 2,009,364 - CA265_RS08080 0.84 +0.1 2,009,364 - CA265_RS08080 0.84 +0.1 2,009,364 - CA265_RS08080 0.84 +0.2 2,009,368 - CA265_RS08080 0.85 -0.4 2,009,368 - CA265_RS08080 0.85 +0.2 2,009,368 - CA265_RS08080 0.85 -0.5 2,009,368 - CA265_RS08080 0.85 -0.3 2,009,413 - +0.7 2,009,422 - +0.3 2,009,429 - +0.4 2,009,433 - -0.2 2,009,436 + -1.9 2,009,545 + CA265_RS08085 0.11 -0.1 2,009,549 + CA265_RS08085 0.12 -1.2 2,009,550 - CA265_RS08085 0.12 -0.3 2,009,552 - CA265_RS08085 0.12 +0.7 2,009,595 - CA265_RS08085 0.16 +0.5 2,009,636 + CA265_RS08085 0.20 -0.1 2,009,636 + CA265_RS08085 0.20 +0.9 2,009,636 + CA265_RS08085 0.20 -0.5 2,009,637 - CA265_RS08085 0.20 +0.6 2,009,637 - CA265_RS08085 0.20 -0.1 2,009,638 + CA265_RS08085 0.20 -0.7 2,009,648 - CA265_RS08085 0.21 -0.9 2,009,648 - CA265_RS08085 0.21 +0.4 2,009,659 - CA265_RS08085 0.22 +1.3 2,009,733 + CA265_RS08085 0.29 -0.1 2,009,775 + CA265_RS08085 0.33 -1.0 2,009,836 - CA265_RS08085 0.39 -0.6 2,009,836 - CA265_RS08085 0.39 +0.6 2,009,836 - CA265_RS08085 0.39 -0.8 2,009,841 + CA265_RS08085 0.39 +0.2 2,009,857 - CA265_RS08085 0.41 +0.3 2,009,898 - CA265_RS08085 0.45 +1.1 2,009,903 - CA265_RS08085 0.45 -0.0 2,009,903 - CA265_RS08085 0.45 -0.6 2,009,999 + CA265_RS08085 0.54 -0.0 2,010,156 + CA265_RS08085 0.69 -1.3 2,010,161 + CA265_RS08085 0.70 -0.9 2,010,166 - CA265_RS08085 0.70 -0.4 2,010,334 - CA265_RS08085 0.86 +0.8
Or see this region's nucleotide sequence