Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33155 and RR42_RS33160 are separated by 95 nucleotides RR42_RS33160 and RR42_RS33165 are separated by 200 nucleotides RR42_RS33165 and RR42_RS33170 are separated by 595 nucleotides
RR42_RS33155: RR42_RS33155 - hypothetical protein, at 2,843,982 to 2,844,161
_RS33155
RR42_RS33160: RR42_RS33160 - nitrogen fixation protein NifQ, at 2,844,257 to 2,844,829
_RS33160
RR42_RS33165: RR42_RS33165 - acyl-CoA thioesterase, at 2,845,030 to 2,845,863
_RS33165
RR42_RS33170: RR42_RS33170 - hypothetical protein, at 2,846,459 to 2,847,580
_RS33170
Position (kb)
2845
2846 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2844.047 kb on + strand, within RR42_RS33155 at 2844.120 kb on + strand, within RR42_RS33155 at 2844.120 kb on + strand, within RR42_RS33155 at 2844.120 kb on + strand, within RR42_RS33155 at 2844.120 kb on + strand, within RR42_RS33155 at 2844.121 kb on - strand, within RR42_RS33155 at 2844.121 kb on - strand, within RR42_RS33155 at 2844.121 kb on - strand, within RR42_RS33155 at 2844.121 kb on - strand, within RR42_RS33155 at 2844.159 kb on + strand at 2844.219 kb on + strand at 2844.220 kb on - strand at 2844.220 kb on - strand at 2844.222 kb on + strand at 2844.300 kb on + strand at 2844.300 kb on + strand at 2844.301 kb on - strand at 2844.301 kb on - strand at 2844.301 kb on - strand at 2844.359 kb on + strand, within RR42_RS33160 at 2844.359 kb on + strand, within RR42_RS33160 at 2844.369 kb on + strand, within RR42_RS33160 at 2844.370 kb on - strand, within RR42_RS33160 at 2844.411 kb on + strand, within RR42_RS33160 at 2844.426 kb on + strand, within RR42_RS33160 at 2844.426 kb on + strand, within RR42_RS33160 at 2844.426 kb on + strand, within RR42_RS33160 at 2844.427 kb on - strand, within RR42_RS33160 at 2844.575 kb on + strand, within RR42_RS33160 at 2844.901 kb on - strand at 2844.901 kb on - strand at 2844.910 kb on + strand at 2844.967 kb on + strand at 2844.967 kb on + strand at 2844.971 kb on + strand at 2844.971 kb on + strand at 2844.971 kb on + strand at 2844.972 kb on - strand at 2844.972 kb on - strand at 2844.972 kb on - strand at 2844.972 kb on - strand at 2844.972 kb on - strand at 2845.026 kb on + strand at 2845.026 kb on + strand at 2845.026 kb on + strand at 2845.026 kb on + strand at 2845.027 kb on - strand at 2845.031 kb on + strand at 2845.031 kb on + strand at 2845.032 kb on - strand at 2845.073 kb on + strand at 2845.073 kb on + strand at 2845.074 kb on - strand at 2845.292 kb on + strand, within RR42_RS33165 at 2845.523 kb on + strand, within RR42_RS33165 at 2845.721 kb on + strand, within RR42_RS33165 at 2845.722 kb on - strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.956 kb on + strand at 2845.956 kb on + strand at 2845.956 kb on + strand at 2845.957 kb on - strand at 2846.034 kb on - strand at 2846.041 kb on + strand at 2846.042 kb on - strand at 2846.049 kb on + strand at 2846.148 kb on + strand at 2846.148 kb on + strand at 2846.148 kb on + strand at 2846.148 kb on + strand at 2846.149 kb on - strand at 2846.149 kb on - strand at 2846.149 kb on - strand at 2846.149 kb on - strand at 2846.190 kb on - strand at 2846.190 kb on - strand at 2846.190 kb on - strand at 2846.250 kb on + strand at 2846.250 kb on + strand at 2846.250 kb on + strand at 2846.251 kb on - strand at 2846.251 kb on - strand at 2846.251 kb on - strand at 2846.253 kb on + strand at 2846.271 kb on + strand at 2846.272 kb on - strand at 2846.272 kb on - strand at 2846.274 kb on + strand at 2846.275 kb on - strand at 2846.275 kb on - strand at 2846.283 kb on + strand at 2846.284 kb on - strand at 2846.284 kb on - strand at 2846.337 kb on - strand at 2846.353 kb on + strand at 2846.433 kb on + strand at 2846.460 kb on + strand at 2846.460 kb on + strand at 2846.461 kb on - strand at 2846.500 kb on - strand at 2846.503 kb on - strand at 2846.535 kb on + strand at 2846.535 kb on + strand at 2846.565 kb on + strand at 2846.565 kb on + strand at 2846.616 kb on + strand, within RR42_RS33170 at 2846.639 kb on - strand, within RR42_RS33170 at 2846.639 kb on - strand, within RR42_RS33170 at 2846.639 kb on - strand, within RR42_RS33170 at 2846.639 kb on - strand, within RR42_RS33170 at 2846.745 kb on + strand, within RR42_RS33170 at 2846.746 kb on - strand, within RR42_RS33170 at 2846.746 kb on - strand, within RR42_RS33170 at 2846.790 kb on + strand, within RR42_RS33170 at 2846.791 kb on - strand, within RR42_RS33170 at 2846.791 kb on - strand, within RR42_RS33170 at 2846.791 kb on - strand, within RR42_RS33170
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 2,844,047 + RR42_RS33155 0.36 -1.3 2,844,120 + RR42_RS33155 0.77 -0.4 2,844,120 + RR42_RS33155 0.77 +0.9 2,844,120 + RR42_RS33155 0.77 +0.5 2,844,120 + RR42_RS33155 0.77 +0.5 2,844,121 - RR42_RS33155 0.77 -0.3 2,844,121 - RR42_RS33155 0.77 -0.4 2,844,121 - RR42_RS33155 0.77 +0.1 2,844,121 - RR42_RS33155 0.77 -1.9 2,844,159 + -0.8 2,844,219 + -0.5 2,844,220 - +0.5 2,844,220 - -0.0 2,844,222 + -0.6 2,844,300 + +0.3 2,844,300 + -0.3 2,844,301 - -0.6 2,844,301 - -1.2 2,844,301 - -0.0 2,844,359 + RR42_RS33160 0.18 -0.0 2,844,359 + RR42_RS33160 0.18 -0.3 2,844,369 + RR42_RS33160 0.20 +0.5 2,844,370 - RR42_RS33160 0.20 +0.1 2,844,411 + RR42_RS33160 0.27 +0.6 2,844,426 + RR42_RS33160 0.29 -0.1 2,844,426 + RR42_RS33160 0.29 -0.2 2,844,426 + RR42_RS33160 0.29 -1.9 2,844,427 - RR42_RS33160 0.30 +1.0 2,844,575 + RR42_RS33160 0.55 -0.6 2,844,901 - +0.4 2,844,901 - -0.8 2,844,910 + +0.5 2,844,967 + +1.9 2,844,967 + +0.6 2,844,971 + +0.2 2,844,971 + -0.9 2,844,971 + -0.1 2,844,972 - +0.4 2,844,972 - -0.2 2,844,972 - -0.8 2,844,972 - -1.4 2,844,972 - +0.7 2,845,026 + -0.5 2,845,026 + -0.5 2,845,026 + -0.5 2,845,026 + +0.5 2,845,027 - -0.6 2,845,031 + -0.6 2,845,031 + -1.4 2,845,032 - +0.1 2,845,073 + +1.0 2,845,073 + +0.0 2,845,074 - +0.1 2,845,292 + RR42_RS33165 0.31 +0.9 2,845,523 + RR42_RS33165 0.59 -0.2 2,845,721 + RR42_RS33165 0.83 -0.1 2,845,722 - RR42_RS33165 0.83 -0.1 2,845,725 + RR42_RS33165 0.83 -0.4 2,845,725 + RR42_RS33165 0.83 +0.4 2,845,725 + RR42_RS33165 0.83 -0.3 2,845,725 + RR42_RS33165 0.83 -0.2 2,845,725 + RR42_RS33165 0.83 -1.1 2,845,726 - RR42_RS33165 0.83 +0.9 2,845,726 - RR42_RS33165 0.83 +0.0 2,845,726 - RR42_RS33165 0.83 +1.2 2,845,726 - RR42_RS33165 0.83 +0.6 2,845,726 - RR42_RS33165 0.83 -0.4 2,845,726 - RR42_RS33165 0.83 -0.2 2,845,726 - RR42_RS33165 0.83 +0.4 2,845,726 - RR42_RS33165 0.83 -0.6 2,845,956 + -0.0 2,845,956 + +0.3 2,845,956 + -0.8 2,845,957 - +0.6 2,846,034 - -0.1 2,846,041 + -1.1 2,846,042 - -2.7 2,846,049 + -1.3 2,846,148 + +0.1 2,846,148 + -1.2 2,846,148 + +0.2 2,846,148 + -0.8 2,846,149 - -0.6 2,846,149 - -0.4 2,846,149 - -1.0 2,846,149 - -1.1 2,846,190 - +1.2 2,846,190 - -2.3 2,846,190 - +1.5 2,846,250 + +0.2 2,846,250 + -1.9 2,846,250 + +0.9 2,846,251 - -0.7 2,846,251 - +1.1 2,846,251 - +1.3 2,846,253 + +1.1 2,846,271 + +1.4 2,846,272 - +1.1 2,846,272 - +2.6 2,846,274 + -0.2 2,846,275 - +1.4 2,846,275 - +1.8 2,846,283 + +0.1 2,846,284 - +1.9 2,846,284 - -0.4 2,846,337 - +1.9 2,846,353 + +0.0 2,846,433 + -0.6 2,846,460 + -1.6 2,846,460 + +0.7 2,846,461 - -0.1 2,846,500 - -0.0 2,846,503 - -0.5 2,846,535 + -1.1 2,846,535 + +0.5 2,846,565 + +0.1 2,846,565 + +0.7 2,846,616 + RR42_RS33170 0.14 -1.7 2,846,639 - RR42_RS33170 0.16 +0.1 2,846,639 - RR42_RS33170 0.16 -0.1 2,846,639 - RR42_RS33170 0.16 +0.3 2,846,639 - RR42_RS33170 0.16 +0.3 2,846,745 + RR42_RS33170 0.25 -2.1 2,846,746 - RR42_RS33170 0.26 -0.5 2,846,746 - RR42_RS33170 0.26 -1.2 2,846,790 + RR42_RS33170 0.30 -0.3 2,846,791 - RR42_RS33170 0.30 -0.1 2,846,791 - RR42_RS33170 0.30 -1.0 2,846,791 - RR42_RS33170 0.30 -1.1
Or see this region's nucleotide sequence