Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32970 and RR42_RS32975 are separated by 152 nucleotides RR42_RS32975 and RR42_RS32980 are separated by 38 nucleotides
RR42_RS32970: RR42_RS32970 - benzoylformate decarboxylase, at 2,796,064 to 2,797,674
_RS32970
RR42_RS32975: RR42_RS32975 - LysR family transcriptional regulator, at 2,797,827 to 2,798,729
_RS32975
RR42_RS32980: RR42_RS32980 - pyruvate dehydrogenase, at 2,798,768 to 2,801,443
_RS32980
Position (kb)
2797
2798
2799 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2796.951 kb on - strand, within RR42_RS32970 at 2796.951 kb on - strand, within RR42_RS32970 at 2797.202 kb on + strand, within RR42_RS32970 at 2797.202 kb on + strand, within RR42_RS32970 at 2797.203 kb on - strand, within RR42_RS32970 at 2797.203 kb on - strand, within RR42_RS32970 at 2797.203 kb on - strand, within RR42_RS32970 at 2797.203 kb on - strand, within RR42_RS32970 at 2797.206 kb on - strand, within RR42_RS32970 at 2797.296 kb on + strand, within RR42_RS32970 at 2797.296 kb on + strand, within RR42_RS32970 at 2797.389 kb on + strand, within RR42_RS32970 at 2797.390 kb on - strand, within RR42_RS32970 at 2797.390 kb on - strand, within RR42_RS32970 at 2797.390 kb on - strand, within RR42_RS32970 at 2797.512 kb on - strand, within RR42_RS32970 at 2797.512 kb on - strand, within RR42_RS32970 at 2797.766 kb on + strand at 2797.766 kb on + strand at 2797.766 kb on + strand at 2797.767 kb on - strand at 2797.767 kb on - strand at 2797.784 kb on - strand at 2797.816 kb on + strand at 2798.334 kb on + strand, within RR42_RS32975 at 2798.335 kb on - strand, within RR42_RS32975 at 2798.640 kb on - strand at 2798.640 kb on - strand at 2798.769 kb on + strand at 2798.770 kb on - strand at 2798.770 kb on - strand at 2798.771 kb on + strand at 2798.772 kb on - strand at 2798.774 kb on - strand at 2798.892 kb on + strand at 2798.893 kb on - strand at 2798.953 kb on - strand at 2799.006 kb on + strand at 2799.006 kb on + strand at 2799.006 kb on + strand at 2799.007 kb on - strand at 2799.007 kb on - strand at 2799.007 kb on - strand at 2799.064 kb on + strand, within RR42_RS32980 at 2799.135 kb on + strand, within RR42_RS32980 at 2799.136 kb on - strand, within RR42_RS32980 at 2799.136 kb on - strand, within RR42_RS32980 at 2799.270 kb on - strand, within RR42_RS32980 at 2799.270 kb on - strand, within RR42_RS32980 at 2799.276 kb on + strand, within RR42_RS32980 at 2799.276 kb on + strand, within RR42_RS32980 at 2799.276 kb on + strand, within RR42_RS32980 at 2799.277 kb on - strand, within RR42_RS32980 at 2799.277 kb on - strand, within RR42_RS32980 at 2799.354 kb on + strand, within RR42_RS32980 at 2799.354 kb on + strand, within RR42_RS32980 at 2799.355 kb on - strand, within RR42_RS32980 at 2799.357 kb on + strand, within RR42_RS32980 at 2799.357 kb on + strand, within RR42_RS32980 at 2799.357 kb on + strand, within RR42_RS32980 at 2799.358 kb on - strand, within RR42_RS32980 at 2799.524 kb on - strand, within RR42_RS32980 at 2799.618 kb on + strand, within RR42_RS32980 at 2799.618 kb on + strand, within RR42_RS32980 at 2799.618 kb on + strand, within RR42_RS32980 at 2799.619 kb on - strand, within RR42_RS32980 at 2799.621 kb on + strand, within RR42_RS32980 at 2799.621 kb on + strand, within RR42_RS32980 at 2799.621 kb on + strand, within RR42_RS32980 at 2799.622 kb on - strand, within RR42_RS32980 at 2799.622 kb on - strand, within RR42_RS32980 at 2799.622 kb on - strand, within RR42_RS32980 at 2799.625 kb on + strand, within RR42_RS32980 at 2799.625 kb on + strand, within RR42_RS32980 at 2799.625 kb on + strand, within RR42_RS32980 at 2799.626 kb on - strand, within RR42_RS32980 at 2799.626 kb on - strand, within RR42_RS32980 at 2799.626 kb on - strand, within RR42_RS32980 at 2799.626 kb on - strand, within RR42_RS32980 at 2799.627 kb on + strand, within RR42_RS32980 at 2799.627 kb on + strand, within RR42_RS32980 at 2799.627 kb on + strand, within RR42_RS32980 at 2799.627 kb on + strand, within RR42_RS32980 at 2799.628 kb on - strand, within RR42_RS32980 at 2799.628 kb on - strand, within RR42_RS32980 at 2799.644 kb on - strand, within RR42_RS32980 at 2799.660 kb on + strand, within RR42_RS32980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 2,796,951 - RR42_RS32970 0.55 -1.7 2,796,951 - RR42_RS32970 0.55 +3.9 2,797,202 + RR42_RS32970 0.71 +1.0 2,797,202 + RR42_RS32970 0.71 -1.0 2,797,203 - RR42_RS32970 0.71 +1.6 2,797,203 - RR42_RS32970 0.71 +0.5 2,797,203 - RR42_RS32970 0.71 -0.5 2,797,203 - RR42_RS32970 0.71 +0.8 2,797,206 - RR42_RS32970 0.71 +1.0 2,797,296 + RR42_RS32970 0.76 +0.7 2,797,296 + RR42_RS32970 0.76 -1.1 2,797,389 + RR42_RS32970 0.82 +0.6 2,797,390 - RR42_RS32970 0.82 +0.1 2,797,390 - RR42_RS32970 0.82 -0.2 2,797,390 - RR42_RS32970 0.82 +0.5 2,797,512 - RR42_RS32970 0.90 +2.9 2,797,512 - RR42_RS32970 0.90 -2.1 2,797,766 + +0.4 2,797,766 + -0.9 2,797,766 + +2.1 2,797,767 - +0.5 2,797,767 - +0.1 2,797,784 - -0.4 2,797,816 + +0.5 2,798,334 + RR42_RS32975 0.56 +0.6 2,798,335 - RR42_RS32975 0.56 -1.7 2,798,640 - +0.5 2,798,640 - -0.7 2,798,769 + +0.2 2,798,770 - +1.1 2,798,770 - -0.1 2,798,771 + +0.4 2,798,772 - -0.8 2,798,774 - +1.3 2,798,892 + +0.7 2,798,893 - +0.2 2,798,953 - +0.6 2,799,006 + -0.9 2,799,006 + -1.3 2,799,006 + +0.1 2,799,007 - -1.5 2,799,007 - +0.1 2,799,007 - -0.1 2,799,064 + RR42_RS32980 0.11 -0.8 2,799,135 + RR42_RS32980 0.14 -0.1 2,799,136 - RR42_RS32980 0.14 -1.3 2,799,136 - RR42_RS32980 0.14 +0.3 2,799,270 - RR42_RS32980 0.19 -1.0 2,799,270 - RR42_RS32980 0.19 +0.3 2,799,276 + RR42_RS32980 0.19 +1.1 2,799,276 + RR42_RS32980 0.19 +0.7 2,799,276 + RR42_RS32980 0.19 +0.3 2,799,277 - RR42_RS32980 0.19 +1.7 2,799,277 - RR42_RS32980 0.19 +0.8 2,799,354 + RR42_RS32980 0.22 -0.5 2,799,354 + RR42_RS32980 0.22 -0.9 2,799,355 - RR42_RS32980 0.22 +0.4 2,799,357 + RR42_RS32980 0.22 -0.2 2,799,357 + RR42_RS32980 0.22 +0.4 2,799,357 + RR42_RS32980 0.22 +0.2 2,799,358 - RR42_RS32980 0.22 -2.5 2,799,524 - RR42_RS32980 0.28 +0.9 2,799,618 + RR42_RS32980 0.32 +0.5 2,799,618 + RR42_RS32980 0.32 -1.3 2,799,618 + RR42_RS32980 0.32 +0.3 2,799,619 - RR42_RS32980 0.32 +0.2 2,799,621 + RR42_RS32980 0.32 +0.5 2,799,621 + RR42_RS32980 0.32 -0.9 2,799,621 + RR42_RS32980 0.32 -0.4 2,799,622 - RR42_RS32980 0.32 -0.5 2,799,622 - RR42_RS32980 0.32 -0.1 2,799,622 - RR42_RS32980 0.32 -0.3 2,799,625 + RR42_RS32980 0.32 -0.5 2,799,625 + RR42_RS32980 0.32 +0.1 2,799,625 + RR42_RS32980 0.32 +0.0 2,799,626 - RR42_RS32980 0.32 +1.4 2,799,626 - RR42_RS32980 0.32 +0.3 2,799,626 - RR42_RS32980 0.32 +1.4 2,799,626 - RR42_RS32980 0.32 -0.6 2,799,627 + RR42_RS32980 0.32 -1.1 2,799,627 + RR42_RS32980 0.32 +2.3 2,799,627 + RR42_RS32980 0.32 -0.8 2,799,627 + RR42_RS32980 0.32 -1.3 2,799,628 - RR42_RS32980 0.32 +0.8 2,799,628 - RR42_RS32980 0.32 +0.1 2,799,644 - RR42_RS32980 0.33 +0.2 2,799,660 + RR42_RS32980 0.33 +0.1
Or see this region's nucleotide sequence