Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32665 and RR42_RS32670 are separated by 15 nucleotides RR42_RS32670 and RR42_RS32675 are separated by 32 nucleotides
RR42_RS32665: RR42_RS32665 - phenol hydroxylase, at 2,724,372 to 2,725,439
_RS32665
RR42_RS32670: RR42_RS32670 - phenol hydroxylase, at 2,725,455 to 2,725,814
_RS32670
RR42_RS32675: RR42_RS32675 - phenol 2-monooxygenase, at 2,725,847 to 2,727,361
_RS32675
Position (kb)
2725
2726 Strain fitness (log2 ratio)
-2
-1
0
1 at 2724.518 kb on - strand, within RR42_RS32665 at 2724.560 kb on - strand, within RR42_RS32665 at 2724.643 kb on + strand, within RR42_RS32665 at 2724.694 kb on + strand, within RR42_RS32665 at 2724.694 kb on + strand, within RR42_RS32665 at 2724.695 kb on - strand, within RR42_RS32665 at 2724.856 kb on + strand, within RR42_RS32665 at 2725.028 kb on - strand, within RR42_RS32665 at 2725.456 kb on + strand at 2725.624 kb on + strand, within RR42_RS32670 at 2725.631 kb on - strand, within RR42_RS32670 at 2725.836 kb on - strand at 2725.951 kb on + strand at 2725.951 kb on + strand at 2725.952 kb on - strand at 2725.952 kb on - strand at 2725.992 kb on + strand at 2725.993 kb on - strand at 2726.095 kb on - strand, within RR42_RS32675 at 2726.095 kb on - strand, within RR42_RS32675 at 2726.340 kb on + strand, within RR42_RS32675 at 2726.341 kb on - strand, within RR42_RS32675 at 2726.520 kb on + strand, within RR42_RS32675 at 2726.521 kb on - strand, within RR42_RS32675 at 2726.521 kb on - strand, within RR42_RS32675 at 2726.545 kb on + strand, within RR42_RS32675 at 2726.545 kb on + strand, within RR42_RS32675 at 2726.546 kb on - strand, within RR42_RS32675 at 2726.656 kb on + strand, within RR42_RS32675 at 2726.657 kb on - strand, within RR42_RS32675 at 2726.766 kb on + strand, within RR42_RS32675
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 2,724,518 - RR42_RS32665 0.14 -0.7 2,724,560 - RR42_RS32665 0.18 +0.6 2,724,643 + RR42_RS32665 0.25 -0.5 2,724,694 + RR42_RS32665 0.30 -0.4 2,724,694 + RR42_RS32665 0.30 -0.5 2,724,695 - RR42_RS32665 0.30 +0.4 2,724,856 + RR42_RS32665 0.45 -0.6 2,725,028 - RR42_RS32665 0.61 +0.7 2,725,456 + -1.8 2,725,624 + RR42_RS32670 0.47 +1.5 2,725,631 - RR42_RS32670 0.49 -0.1 2,725,836 - +0.8 2,725,951 + -0.6 2,725,951 + -0.6 2,725,952 - +0.6 2,725,952 - +0.3 2,725,992 + -1.7 2,725,993 - +0.2 2,726,095 - RR42_RS32675 0.16 +0.3 2,726,095 - RR42_RS32675 0.16 -0.6 2,726,340 + RR42_RS32675 0.33 +0.4 2,726,341 - RR42_RS32675 0.33 +0.9 2,726,520 + RR42_RS32675 0.44 -0.5 2,726,521 - RR42_RS32675 0.44 +0.7 2,726,521 - RR42_RS32675 0.44 -0.3 2,726,545 + RR42_RS32675 0.46 -2.7 2,726,545 + RR42_RS32675 0.46 -0.7 2,726,546 - RR42_RS32675 0.46 -1.8 2,726,656 + RR42_RS32675 0.53 +0.5 2,726,657 - RR42_RS32675 0.53 -0.4 2,726,766 + RR42_RS32675 0.61 -0.1
Or see this region's nucleotide sequence