Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32005 and RR42_RS32010 are separated by 121 nucleotides RR42_RS32010 and RR42_RS32015 overlap by 16 nucleotides RR42_RS32015 and RR42_RS32020 are separated by 56 nucleotides
RR42_RS32005: RR42_RS32005 - flavin-dependent monooxygenase, at 2,558,662 to 2,559,855
_RS32005
RR42_RS32010: RR42_RS32010 - 5,10-methylene tetrahydromethanopterin reductase, at 2,559,977 to 2,561,128
_RS32010
RR42_RS32015: RR42_RS32015 - hypothetical protein, at 2,561,113 to 2,561,301
_RS32015
RR42_RS32020: RR42_RS32020 - hypothetical protein, at 2,561,358 to 2,561,966
_RS32020
Position (kb)
2559
2560
2561
2562 Strain fitness (log2 ratio)
-2
-1
0
1 at 2559.068 kb on + strand, within RR42_RS32005 at 2559.068 kb on + strand, within RR42_RS32005 at 2559.158 kb on + strand, within RR42_RS32005 at 2559.318 kb on - strand, within RR42_RS32005 at 2559.347 kb on + strand, within RR42_RS32005 at 2559.489 kb on + strand, within RR42_RS32005 at 2559.491 kb on + strand, within RR42_RS32005 at 2559.492 kb on - strand, within RR42_RS32005 at 2559.510 kb on + strand, within RR42_RS32005 at 2559.593 kb on + strand, within RR42_RS32005 at 2559.594 kb on - strand, within RR42_RS32005 at 2559.840 kb on - strand at 2559.944 kb on + strand at 2560.326 kb on + strand, within RR42_RS32010 at 2560.333 kb on - strand, within RR42_RS32010 at 2560.480 kb on - strand, within RR42_RS32010 at 2560.491 kb on + strand, within RR42_RS32010 at 2560.602 kb on + strand, within RR42_RS32010 at 2560.603 kb on - strand, within RR42_RS32010 at 2560.665 kb on + strand, within RR42_RS32010 at 2560.975 kb on - strand, within RR42_RS32010 at 2560.975 kb on - strand, within RR42_RS32010 at 2561.109 kb on + strand at 2561.110 kb on - strand at 2561.284 kb on + strand at 2561.364 kb on - strand at 2561.485 kb on - strand, within RR42_RS32020 at 2561.618 kb on + strand, within RR42_RS32020 at 2561.619 kb on - strand, within RR42_RS32020 at 2561.652 kb on + strand, within RR42_RS32020 at 2561.652 kb on + strand, within RR42_RS32020 at 2561.652 kb on + strand, within RR42_RS32020 at 2561.652 kb on + strand, within RR42_RS32020 at 2561.652 kb on + strand, within RR42_RS32020 at 2561.653 kb on - strand, within RR42_RS32020 at 2561.653 kb on - strand, within RR42_RS32020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 2,559,068 + RR42_RS32005 0.34 +0.7 2,559,068 + RR42_RS32005 0.34 -0.8 2,559,158 + RR42_RS32005 0.42 -1.1 2,559,318 - RR42_RS32005 0.55 -0.8 2,559,347 + RR42_RS32005 0.57 +0.2 2,559,489 + RR42_RS32005 0.69 -0.5 2,559,491 + RR42_RS32005 0.69 -1.4 2,559,492 - RR42_RS32005 0.70 -0.3 2,559,510 + RR42_RS32005 0.71 -1.5 2,559,593 + RR42_RS32005 0.78 -0.7 2,559,594 - RR42_RS32005 0.78 -0.3 2,559,840 - -0.5 2,559,944 + +0.1 2,560,326 + RR42_RS32010 0.30 +0.8 2,560,333 - RR42_RS32010 0.31 +0.2 2,560,480 - RR42_RS32010 0.44 -0.2 2,560,491 + RR42_RS32010 0.45 +1.5 2,560,602 + RR42_RS32010 0.54 -0.6 2,560,603 - RR42_RS32010 0.54 +0.3 2,560,665 + RR42_RS32010 0.60 -0.4 2,560,975 - RR42_RS32010 0.87 +0.2 2,560,975 - RR42_RS32010 0.87 -0.4 2,561,109 + -1.0 2,561,110 - +0.6 2,561,284 + -0.1 2,561,364 - -0.3 2,561,485 - RR42_RS32020 0.21 -0.8 2,561,618 + RR42_RS32020 0.43 -0.7 2,561,619 - RR42_RS32020 0.43 -1.9 2,561,652 + RR42_RS32020 0.48 -0.8 2,561,652 + RR42_RS32020 0.48 -0.0 2,561,652 + RR42_RS32020 0.48 -0.8 2,561,652 + RR42_RS32020 0.48 -0.7 2,561,652 + RR42_RS32020 0.48 +1.0 2,561,653 - RR42_RS32020 0.48 -0.1 2,561,653 - RR42_RS32020 0.48 +0.6
Or see this region's nucleotide sequence