Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31245 and RR42_RS31250 are separated by 6 nucleotides RR42_RS31250 and RR42_RS31255 are separated by 87 nucleotides RR42_RS31255 and RR42_RS31260 overlap by 14 nucleotides RR42_RS31260 and RR42_RS31265 overlap by 8 nucleotides
RR42_RS31245: RR42_RS31245 - membrane protein, at 2,386,811 to 2,387,359
_RS31245
RR42_RS31250: RR42_RS31250 - acyl carrier protein, at 2,387,366 to 2,387,623
_RS31250
RR42_RS31255: RR42_RS31255 - acyl carrier protein, at 2,387,711 to 2,387,986
_RS31255
RR42_RS31260: RR42_RS31260 - acyltransferase, at 2,387,973 to 2,388,749
_RS31260
RR42_RS31265: RR42_RS31265 - hypothetical protein, at 2,388,742 to 2,389,470
_RS31265
Position (kb)
2387
2388
2389 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2387.301 kb on + strand, within RR42_RS31245 at 2387.301 kb on + strand, within RR42_RS31245 at 2387.317 kb on - strand at 2387.681 kb on - strand at 2387.681 kb on - strand at 2387.681 kb on - strand at 2388.016 kb on - strand at 2388.109 kb on + strand, within RR42_RS31260 at 2388.109 kb on + strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.358 kb on + strand, within RR42_RS31260 at 2388.359 kb on - strand, within RR42_RS31260 at 2388.533 kb on - strand, within RR42_RS31260 at 2388.583 kb on + strand, within RR42_RS31260 at 2388.584 kb on - strand, within RR42_RS31260 at 2388.974 kb on + strand, within RR42_RS31265 at 2388.974 kb on + strand, within RR42_RS31265 at 2388.974 kb on + strand, within RR42_RS31265 at 2388.975 kb on - strand, within RR42_RS31265 at 2388.998 kb on + strand, within RR42_RS31265 at 2388.998 kb on + strand, within RR42_RS31265 at 2388.999 kb on - strand, within RR42_RS31265 at 2389.127 kb on + strand, within RR42_RS31265 at 2389.147 kb on - strand, within RR42_RS31265 at 2389.286 kb on + strand, within RR42_RS31265 at 2389.286 kb on + strand, within RR42_RS31265 at 2389.286 kb on + strand, within RR42_RS31265 at 2389.287 kb on - strand, within RR42_RS31265 at 2389.427 kb on - strand at 2389.427 kb on - strand at 2389.427 kb on - strand at 2389.487 kb on + strand at 2389.488 kb on - strand at 2389.488 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 2,387,301 + RR42_RS31245 0.89 +0.6 2,387,301 + RR42_RS31245 0.89 -0.4 2,387,317 - +0.3 2,387,681 - -1.0 2,387,681 - +2.0 2,387,681 - -0.1 2,388,016 - +0.7 2,388,109 + RR42_RS31260 0.18 -3.0 2,388,109 + RR42_RS31260 0.18 -1.1 2,388,110 - RR42_RS31260 0.18 -0.4 2,388,110 - RR42_RS31260 0.18 -1.2 2,388,110 - RR42_RS31260 0.18 -0.1 2,388,110 - RR42_RS31260 0.18 +0.0 2,388,358 + RR42_RS31260 0.50 -0.6 2,388,359 - RR42_RS31260 0.50 -1.1 2,388,533 - RR42_RS31260 0.72 +0.1 2,388,583 + RR42_RS31260 0.79 +0.5 2,388,584 - RR42_RS31260 0.79 +0.7 2,388,974 + RR42_RS31265 0.32 -0.4 2,388,974 + RR42_RS31265 0.32 -0.7 2,388,974 + RR42_RS31265 0.32 -0.1 2,388,975 - RR42_RS31265 0.32 -0.3 2,388,998 + RR42_RS31265 0.35 -0.4 2,388,998 + RR42_RS31265 0.35 -0.4 2,388,999 - RR42_RS31265 0.35 -1.6 2,389,127 + RR42_RS31265 0.53 -1.5 2,389,147 - RR42_RS31265 0.56 +0.7 2,389,286 + RR42_RS31265 0.75 -0.4 2,389,286 + RR42_RS31265 0.75 -1.0 2,389,286 + RR42_RS31265 0.75 -1.9 2,389,287 - RR42_RS31265 0.75 -1.3 2,389,427 - -2.2 2,389,427 - -0.9 2,389,427 - -0.0 2,389,487 + -1.6 2,389,488 - +2.1 2,389,488 - -0.9
Or see this region's nucleotide sequence