Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29245 and RR42_RS29250 overlap by 8 nucleotides RR42_RS29250 and RR42_RS29255 are separated by 174 nucleotides RR42_RS29255 and RR42_RS29260 are separated by 129 nucleotides
RR42_RS29245: RR42_RS29245 - nitrile hydratase, at 1,915,672 to 1,916,343
_RS29245
RR42_RS29250: RR42_RS29250 - nitrile hydratase, at 1,916,336 to 1,916,722
_RS29250
RR42_RS29255: RR42_RS29255 - GTP cyclohydrolase, at 1,916,897 to 1,917,493
_RS29255
RR42_RS29260: RR42_RS29260 - hypothetical protein, at 1,917,623 to 1,918,972
_RS29260
Position (kb)
1916
1917
1918 Strain fitness (log2 ratio)
-2
-1
0
1 at 1916.131 kb on + strand, within RR42_RS29245 at 1916.131 kb on + strand, within RR42_RS29245 at 1916.132 kb on - strand, within RR42_RS29245 at 1916.230 kb on + strand at 1916.230 kb on + strand, within RR42_RS29245 at 1916.231 kb on - strand, within RR42_RS29245 at 1916.311 kb on + strand at 1916.312 kb on - strand at 1916.312 kb on - strand at 1916.312 kb on - strand at 1916.579 kb on + strand, within RR42_RS29250 at 1916.579 kb on + strand, within RR42_RS29250 at 1916.579 kb on + strand, within RR42_RS29250 at 1916.579 kb on + strand, within RR42_RS29250 at 1916.580 kb on - strand, within RR42_RS29250 at 1916.580 kb on - strand, within RR42_RS29250 at 1916.580 kb on - strand, within RR42_RS29250 at 1916.582 kb on + strand, within RR42_RS29250 at 1916.583 kb on - strand, within RR42_RS29250 at 1916.583 kb on - strand, within RR42_RS29250 at 1916.744 kb on + strand at 1916.825 kb on - strand at 1916.837 kb on + strand at 1916.845 kb on - strand at 1916.960 kb on - strand, within RR42_RS29255 at 1916.960 kb on - strand, within RR42_RS29255 at 1917.164 kb on + strand, within RR42_RS29255 at 1917.164 kb on + strand, within RR42_RS29255 at 1917.218 kb on + strand, within RR42_RS29255 at 1917.219 kb on - strand, within RR42_RS29255 at 1917.219 kb on - strand, within RR42_RS29255 at 1917.245 kb on - strand, within RR42_RS29255 at 1917.293 kb on + strand, within RR42_RS29255 at 1917.294 kb on - strand, within RR42_RS29255 at 1917.294 kb on - strand, within RR42_RS29255 at 1917.418 kb on + strand, within RR42_RS29255 at 1917.418 kb on + strand, within RR42_RS29255 at 1917.419 kb on - strand, within RR42_RS29255 at 1917.757 kb on + strand at 1917.777 kb on - strand, within RR42_RS29260 at 1917.810 kb on - strand, within RR42_RS29260 at 1917.924 kb on - strand, within RR42_RS29260 at 1917.947 kb on + strand, within RR42_RS29260 at 1917.947 kb on + strand, within RR42_RS29260 at 1917.947 kb on + strand, within RR42_RS29260 at 1917.947 kb on + strand, within RR42_RS29260 at 1917.948 kb on - strand, within RR42_RS29260 at 1917.948 kb on - strand, within RR42_RS29260 at 1917.948 kb on - strand, within RR42_RS29260 at 1917.953 kb on + strand, within RR42_RS29260 at 1917.953 kb on + strand, within RR42_RS29260 at 1917.953 kb on + strand, within RR42_RS29260 at 1917.954 kb on - strand, within RR42_RS29260 at 1917.954 kb on - strand, within RR42_RS29260 at 1917.954 kb on - strand, within RR42_RS29260 at 1917.954 kb on - strand, within RR42_RS29260 at 1917.954 kb on - strand, within RR42_RS29260 at 1917.954 kb on - strand, within RR42_RS29260 at 1917.954 kb on - strand, within RR42_RS29260 at 1917.954 kb on - strand, within RR42_RS29260 at 1917.954 kb on - strand, within RR42_RS29260 at 1918.085 kb on + strand, within RR42_RS29260 at 1918.086 kb on - strand, within RR42_RS29260 at 1918.091 kb on + strand, within RR42_RS29260 at 1918.091 kb on + strand, within RR42_RS29260 at 1918.091 kb on + strand, within RR42_RS29260 at 1918.091 kb on + strand, within RR42_RS29260 at 1918.092 kb on - strand, within RR42_RS29260 at 1918.092 kb on - strand, within RR42_RS29260 at 1918.335 kb on - strand, within RR42_RS29260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 1,916,131 + RR42_RS29245 0.68 +0.8 1,916,131 + RR42_RS29245 0.68 -0.2 1,916,132 - RR42_RS29245 0.68 -0.0 1,916,230 + +1.6 1,916,230 + RR42_RS29245 0.83 -1.3 1,916,231 - RR42_RS29245 0.83 -1.3 1,916,311 + +0.3 1,916,312 - -1.4 1,916,312 - -2.3 1,916,312 - -0.6 1,916,579 + RR42_RS29250 0.63 +0.3 1,916,579 + RR42_RS29250 0.63 +1.0 1,916,579 + RR42_RS29250 0.63 -0.1 1,916,579 + RR42_RS29250 0.63 +1.5 1,916,580 - RR42_RS29250 0.63 +0.4 1,916,580 - RR42_RS29250 0.63 +0.1 1,916,580 - RR42_RS29250 0.63 +0.4 1,916,582 + RR42_RS29250 0.64 -2.0 1,916,583 - RR42_RS29250 0.64 +0.9 1,916,583 - RR42_RS29250 0.64 -0.7 1,916,744 + -0.9 1,916,825 - +0.6 1,916,837 + +0.8 1,916,845 - -1.2 1,916,960 - RR42_RS29255 0.11 -2.2 1,916,960 - RR42_RS29255 0.11 +0.1 1,917,164 + RR42_RS29255 0.45 -0.5 1,917,164 + RR42_RS29255 0.45 -0.6 1,917,218 + RR42_RS29255 0.54 +1.2 1,917,219 - RR42_RS29255 0.54 +0.8 1,917,219 - RR42_RS29255 0.54 -1.4 1,917,245 - RR42_RS29255 0.58 -1.7 1,917,293 + RR42_RS29255 0.66 +0.6 1,917,294 - RR42_RS29255 0.66 +0.8 1,917,294 - RR42_RS29255 0.66 -0.3 1,917,418 + RR42_RS29255 0.87 +0.8 1,917,418 + RR42_RS29255 0.87 -0.5 1,917,419 - RR42_RS29255 0.87 +1.6 1,917,757 + -0.4 1,917,777 - RR42_RS29260 0.11 +1.6 1,917,810 - RR42_RS29260 0.14 -1.4 1,917,924 - RR42_RS29260 0.22 -0.1 1,917,947 + RR42_RS29260 0.24 -1.4 1,917,947 + RR42_RS29260 0.24 -0.1 1,917,947 + RR42_RS29260 0.24 -0.9 1,917,947 + RR42_RS29260 0.24 -1.0 1,917,948 - RR42_RS29260 0.24 -1.1 1,917,948 - RR42_RS29260 0.24 +1.2 1,917,948 - RR42_RS29260 0.24 -0.4 1,917,953 + RR42_RS29260 0.24 -0.2 1,917,953 + RR42_RS29260 0.24 -0.3 1,917,953 + RR42_RS29260 0.24 -0.4 1,917,954 - RR42_RS29260 0.25 +0.0 1,917,954 - RR42_RS29260 0.25 -0.0 1,917,954 - RR42_RS29260 0.25 -2.1 1,917,954 - RR42_RS29260 0.25 -1.0 1,917,954 - RR42_RS29260 0.25 +0.4 1,917,954 - RR42_RS29260 0.25 -1.7 1,917,954 - RR42_RS29260 0.25 +0.0 1,917,954 - RR42_RS29260 0.25 +0.4 1,917,954 - RR42_RS29260 0.25 +0.2 1,918,085 + RR42_RS29260 0.34 +1.6 1,918,086 - RR42_RS29260 0.34 +0.0 1,918,091 + RR42_RS29260 0.35 +0.9 1,918,091 + RR42_RS29260 0.35 -0.7 1,918,091 + RR42_RS29260 0.35 -1.7 1,918,091 + RR42_RS29260 0.35 +1.2 1,918,092 - RR42_RS29260 0.35 -0.5 1,918,092 - RR42_RS29260 0.35 -0.6 1,918,335 - RR42_RS29260 0.53 -1.1
Or see this region's nucleotide sequence