Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29115 and RR42_RS29120 are separated by 8 nucleotides RR42_RS29120 and RR42_RS29125 are separated by 176 nucleotides RR42_RS29125 and RR42_RS29130 are separated by 45 nucleotides
RR42_RS29115: RR42_RS29115 - LysR family transcriptional regulator, at 1,888,706 to 1,889,632
_RS29115
RR42_RS29120: RR42_RS29120 - alpha-dehydro-beta-deoxy-D-glucarate aldolase, at 1,889,641 to 1,890,408
_RS29120
RR42_RS29125: RR42_RS29125 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,890,585 to 1,890,959
_RS29125
RR42_RS29130: RR42_RS29130 - dehydrogenase, at 1,891,005 to 1,892,099
_RS29130
Position (kb)
1889
1890
1891 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1888.652 kb on - strand at 1888.748 kb on - strand at 1888.748 kb on - strand at 1888.961 kb on - strand, within RR42_RS29115 at 1889.193 kb on + strand, within RR42_RS29115 at 1889.194 kb on - strand, within RR42_RS29115 at 1889.204 kb on + strand, within RR42_RS29115 at 1889.205 kb on - strand, within RR42_RS29115 at 1889.434 kb on - strand, within RR42_RS29115 at 1889.468 kb on + strand, within RR42_RS29115 at 1889.537 kb on + strand, within RR42_RS29115 at 1889.537 kb on + strand, within RR42_RS29115 at 1889.976 kb on + strand, within RR42_RS29120 at 1889.976 kb on + strand, within RR42_RS29120 at 1889.977 kb on - strand, within RR42_RS29120 at 1889.977 kb on - strand, within RR42_RS29120 at 1889.977 kb on - strand, within RR42_RS29120 at 1889.977 kb on - strand, within RR42_RS29120 at 1889.999 kb on + strand, within RR42_RS29120 at 1890.000 kb on - strand, within RR42_RS29120 at 1890.043 kb on + strand, within RR42_RS29120 at 1891.283 kb on + strand, within RR42_RS29130 at 1891.283 kb on + strand, within RR42_RS29130 at 1891.284 kb on - strand, within RR42_RS29130 at 1891.284 kb on - strand, within RR42_RS29130 at 1891.384 kb on + strand, within RR42_RS29130 at 1891.384 kb on + strand, within RR42_RS29130 at 1891.385 kb on - strand, within RR42_RS29130 at 1891.385 kb on - strand, within RR42_RS29130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 1,888,652 - +2.1 1,888,748 - +1.6 1,888,748 - +1.0 1,888,961 - RR42_RS29115 0.28 -0.3 1,889,193 + RR42_RS29115 0.53 -0.9 1,889,194 - RR42_RS29115 0.53 +1.6 1,889,204 + RR42_RS29115 0.54 -0.5 1,889,205 - RR42_RS29115 0.54 -0.7 1,889,434 - RR42_RS29115 0.79 +0.4 1,889,468 + RR42_RS29115 0.82 -0.9 1,889,537 + RR42_RS29115 0.90 -0.4 1,889,537 + RR42_RS29115 0.90 -0.4 1,889,976 + RR42_RS29120 0.44 -1.6 1,889,976 + RR42_RS29120 0.44 +0.4 1,889,977 - RR42_RS29120 0.44 +1.2 1,889,977 - RR42_RS29120 0.44 +2.6 1,889,977 - RR42_RS29120 0.44 -1.7 1,889,977 - RR42_RS29120 0.44 -0.2 1,889,999 + RR42_RS29120 0.47 +0.4 1,890,000 - RR42_RS29120 0.47 -2.6 1,890,043 + RR42_RS29120 0.52 -1.0 1,891,283 + RR42_RS29130 0.25 +0.4 1,891,283 + RR42_RS29130 0.25 +0.6 1,891,284 - RR42_RS29130 0.25 +0.5 1,891,284 - RR42_RS29130 0.25 -0.4 1,891,384 + RR42_RS29130 0.35 -0.1 1,891,384 + RR42_RS29130 0.35 +0.9 1,891,385 - RR42_RS29130 0.35 +0.2 1,891,385 - RR42_RS29130 0.35 -2.3
Or see this region's nucleotide sequence