Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28950 and RR42_RS28955 are separated by 610 nucleotides RR42_RS28955 and RR42_RS28960 are separated by 192 nucleotides RR42_RS28960 and RR42_RS28965 are separated by 55 nucleotides
RR42_RS28950: RR42_RS28950 - ammonium transporter, at 1,853,412 to 1,854,614
_RS28950
RR42_RS28955: RR42_RS28955 - transcriptional regulator, at 1,855,225 to 1,855,506
_RS28955
RR42_RS28960: RR42_RS28960 - acyl-CoA hydrolase, at 1,855,699 to 1,856,184
_RS28960
RR42_RS28965: RR42_RS28965 - hypothetical protein, at 1,856,240 to 1,856,707
_RS28965
Position (kb)
1855
1856 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1854.232 kb on + strand, within RR42_RS28950 at 1854.233 kb on - strand, within RR42_RS28950 at 1854.308 kb on + strand, within RR42_RS28950 at 1854.308 kb on + strand, within RR42_RS28950 at 1854.318 kb on + strand, within RR42_RS28950 at 1854.318 kb on + strand, within RR42_RS28950 at 1854.364 kb on + strand, within RR42_RS28950 at 1854.364 kb on + strand, within RR42_RS28950 at 1854.365 kb on - strand, within RR42_RS28950 at 1854.365 kb on - strand, within RR42_RS28950 at 1854.421 kb on - strand, within RR42_RS28950 at 1854.421 kb on - strand, within RR42_RS28950 at 1854.421 kb on - strand, within RR42_RS28950 at 1854.421 kb on - strand, within RR42_RS28950 at 1854.434 kb on + strand, within RR42_RS28950 at 1854.435 kb on - strand, within RR42_RS28950 at 1854.435 kb on - strand, within RR42_RS28950 at 1854.474 kb on + strand, within RR42_RS28950 at 1854.475 kb on - strand, within RR42_RS28950 at 1854.475 kb on - strand, within RR42_RS28950 at 1854.475 kb on - strand, within RR42_RS28950 at 1854.570 kb on + strand at 1854.614 kb on + strand at 1854.614 kb on + strand at 1854.615 kb on - strand at 1854.615 kb on - strand at 1854.615 kb on - strand at 1854.615 kb on - strand at 1854.615 kb on - strand at 1854.624 kb on + strand at 1854.625 kb on - strand at 1854.660 kb on + strand at 1854.661 kb on - strand at 1854.800 kb on + strand at 1854.801 kb on - strand at 1854.834 kb on - strand at 1854.999 kb on - strand at 1854.999 kb on - strand at 1854.999 kb on - strand at 1855.141 kb on + strand at 1855.141 kb on + strand at 1855.142 kb on - strand at 1855.142 kb on - strand at 1855.142 kb on - strand at 1855.153 kb on + strand at 1855.212 kb on - strand at 1855.212 kb on - strand at 1855.231 kb on - strand at 1855.231 kb on - strand at 1855.231 kb on - strand at 1855.245 kb on - strand at 1855.245 kb on - strand at 1855.320 kb on + strand, within RR42_RS28955 at 1855.321 kb on - strand, within RR42_RS28955 at 1855.321 kb on - strand, within RR42_RS28955 at 1855.398 kb on + strand, within RR42_RS28955 at 1855.398 kb on + strand, within RR42_RS28955 at 1855.416 kb on + strand, within RR42_RS28955 at 1855.417 kb on - strand, within RR42_RS28955 at 1855.505 kb on - strand at 1855.505 kb on - strand at 1855.559 kb on + strand at 1855.560 kb on - strand at 1855.795 kb on + strand, within RR42_RS28960 at 1855.795 kb on + strand, within RR42_RS28960 at 1855.796 kb on - strand, within RR42_RS28960 at 1855.813 kb on + strand, within RR42_RS28960 at 1855.813 kb on + strand, within RR42_RS28960 at 1855.813 kb on + strand, within RR42_RS28960 at 1855.813 kb on + strand, within RR42_RS28960 at 1855.813 kb on + strand, within RR42_RS28960 at 1855.843 kb on + strand, within RR42_RS28960 at 1855.980 kb on + strand, within RR42_RS28960 at 1856.018 kb on - strand, within RR42_RS28960 at 1856.018 kb on - strand, within RR42_RS28960 at 1856.018 kb on - strand, within RR42_RS28960 at 1856.399 kb on + strand, within RR42_RS28965 at 1856.400 kb on - strand, within RR42_RS28965 at 1856.400 kb on - strand, within RR42_RS28965 at 1856.400 kb on - strand, within RR42_RS28965
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 1,854,232 + RR42_RS28950 0.68 -1.4 1,854,233 - RR42_RS28950 0.68 +1.6 1,854,308 + RR42_RS28950 0.74 -0.1 1,854,308 + RR42_RS28950 0.74 -0.2 1,854,318 + RR42_RS28950 0.75 +1.4 1,854,318 + RR42_RS28950 0.75 -0.6 1,854,364 + RR42_RS28950 0.79 -0.6 1,854,364 + RR42_RS28950 0.79 -1.5 1,854,365 - RR42_RS28950 0.79 +0.2 1,854,365 - RR42_RS28950 0.79 +0.2 1,854,421 - RR42_RS28950 0.84 +1.8 1,854,421 - RR42_RS28950 0.84 +0.5 1,854,421 - RR42_RS28950 0.84 -0.3 1,854,421 - RR42_RS28950 0.84 +1.0 1,854,434 + RR42_RS28950 0.85 -0.1 1,854,435 - RR42_RS28950 0.85 -0.4 1,854,435 - RR42_RS28950 0.85 +0.3 1,854,474 + RR42_RS28950 0.88 -1.0 1,854,475 - RR42_RS28950 0.88 +0.6 1,854,475 - RR42_RS28950 0.88 -0.6 1,854,475 - RR42_RS28950 0.88 +0.8 1,854,570 + +0.5 1,854,614 + -1.5 1,854,614 + -0.5 1,854,615 - +0.0 1,854,615 - -0.8 1,854,615 - -1.0 1,854,615 - -0.5 1,854,615 - -0.7 1,854,624 + -0.3 1,854,625 - +0.4 1,854,660 + +0.6 1,854,661 - -0.4 1,854,800 + +0.4 1,854,801 - +0.2 1,854,834 - +0.6 1,854,999 - -1.3 1,854,999 - +0.6 1,854,999 - +0.7 1,855,141 + -0.2 1,855,141 + -0.0 1,855,142 - +0.7 1,855,142 - +0.4 1,855,142 - +2.0 1,855,153 + +0.2 1,855,212 - +1.6 1,855,212 - +0.2 1,855,231 - -1.0 1,855,231 - +1.3 1,855,231 - +0.0 1,855,245 - +0.0 1,855,245 - -0.8 1,855,320 + RR42_RS28955 0.34 +0.8 1,855,321 - RR42_RS28955 0.34 +0.4 1,855,321 - RR42_RS28955 0.34 -0.6 1,855,398 + RR42_RS28955 0.61 +1.2 1,855,398 + RR42_RS28955 0.61 +0.2 1,855,416 + RR42_RS28955 0.68 +0.6 1,855,417 - RR42_RS28955 0.68 +1.0 1,855,505 - -2.5 1,855,505 - -1.2 1,855,559 + +0.6 1,855,560 - +1.0 1,855,795 + RR42_RS28960 0.20 +0.6 1,855,795 + RR42_RS28960 0.20 +0.5 1,855,796 - RR42_RS28960 0.20 +0.6 1,855,813 + RR42_RS28960 0.23 +0.9 1,855,813 + RR42_RS28960 0.23 -0.9 1,855,813 + RR42_RS28960 0.23 -2.5 1,855,813 + RR42_RS28960 0.23 +0.2 1,855,813 + RR42_RS28960 0.23 +0.4 1,855,843 + RR42_RS28960 0.30 +1.1 1,855,980 + RR42_RS28960 0.58 +0.5 1,856,018 - RR42_RS28960 0.66 +0.9 1,856,018 - RR42_RS28960 0.66 +0.4 1,856,018 - RR42_RS28960 0.66 -0.1 1,856,399 + RR42_RS28965 0.34 -0.4 1,856,400 - RR42_RS28965 0.34 +2.4 1,856,400 - RR42_RS28965 0.34 -0.5 1,856,400 - RR42_RS28965 0.34 -0.1
Or see this region's nucleotide sequence