Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27845 and RR42_RS27850 are separated by 63 nucleotides RR42_RS27850 and RR42_RS27855 are separated by 21 nucleotides RR42_RS27855 and RR42_RS27860 are separated by 116 nucleotides
RR42_RS27845: RR42_RS27845 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,611,120 to 1,611,509
_RS27845
RR42_RS27850: RR42_RS27850 - 2-oxo-hepta-3-ene-1,7-dioate hydratase, at 1,611,573 to 1,612,376
_RS27850
RR42_RS27855: RR42_RS27855 - 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase, at 1,612,398 to 1,613,201
_RS27855
RR42_RS27860: RR42_RS27860 - ABC transporter substrate-binding protein, at 1,613,318 to 1,614,283
_RS27860
Position (kb)
1612
1613
1614 Strain fitness (log2 ratio)
-1
0
1 at 1611.406 kb on + strand, within RR42_RS27845 at 1611.406 kb on + strand, within RR42_RS27845 at 1611.406 kb on + strand, within RR42_RS27845 at 1611.407 kb on - strand, within RR42_RS27845 at 1611.409 kb on - strand, within RR42_RS27845 at 1611.409 kb on - strand, within RR42_RS27845 at 1611.468 kb on + strand, within RR42_RS27845 at 1611.690 kb on + strand, within RR42_RS27850 at 1611.811 kb on - strand, within RR42_RS27850 at 1611.954 kb on + strand, within RR42_RS27850 at 1611.954 kb on + strand, within RR42_RS27850 at 1611.955 kb on - strand, within RR42_RS27850 at 1611.969 kb on + strand, within RR42_RS27850 at 1611.969 kb on + strand, within RR42_RS27850 at 1612.322 kb on + strand at 1612.415 kb on + strand at 1612.482 kb on + strand, within RR42_RS27855 at 1612.483 kb on - strand, within RR42_RS27855 at 1613.071 kb on - strand, within RR42_RS27855 at 1613.197 kb on - strand at 1613.199 kb on + strand at 1613.200 kb on - strand at 1613.420 kb on + strand, within RR42_RS27860 at 1613.421 kb on - strand, within RR42_RS27860 at 1613.596 kb on + strand, within RR42_RS27860 at 1613.597 kb on - strand, within RR42_RS27860 at 1613.604 kb on + strand, within RR42_RS27860 at 1613.605 kb on - strand, within RR42_RS27860 at 1613.608 kb on + strand, within RR42_RS27860 at 1613.609 kb on - strand, within RR42_RS27860 at 1613.609 kb on - strand, within RR42_RS27860 at 1613.774 kb on + strand, within RR42_RS27860 at 1613.774 kb on + strand, within RR42_RS27860 at 1613.862 kb on - strand, within RR42_RS27860
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 1,611,406 + RR42_RS27845 0.73 -0.8 1,611,406 + RR42_RS27845 0.73 -1.6 1,611,406 + RR42_RS27845 0.73 +0.1 1,611,407 - RR42_RS27845 0.74 -1.2 1,611,409 - RR42_RS27845 0.74 +0.1 1,611,409 - RR42_RS27845 0.74 +0.3 1,611,468 + RR42_RS27845 0.89 -1.3 1,611,690 + RR42_RS27850 0.15 -0.5 1,611,811 - RR42_RS27850 0.30 +1.5 1,611,954 + RR42_RS27850 0.47 -1.5 1,611,954 + RR42_RS27850 0.47 +1.0 1,611,955 - RR42_RS27850 0.48 -0.8 1,611,969 + RR42_RS27850 0.49 -0.1 1,611,969 + RR42_RS27850 0.49 +0.3 1,612,322 + +0.3 1,612,415 + -1.1 1,612,482 + RR42_RS27855 0.10 -1.3 1,612,483 - RR42_RS27855 0.11 +0.6 1,613,071 - RR42_RS27855 0.84 -0.3 1,613,197 - +1.7 1,613,199 + -1.6 1,613,200 - -0.6 1,613,420 + RR42_RS27860 0.11 -0.8 1,613,421 - RR42_RS27860 0.11 -0.6 1,613,596 + RR42_RS27860 0.29 -0.4 1,613,597 - RR42_RS27860 0.29 +0.2 1,613,604 + RR42_RS27860 0.30 +1.2 1,613,605 - RR42_RS27860 0.30 +0.1 1,613,608 + RR42_RS27860 0.30 +0.7 1,613,609 - RR42_RS27860 0.30 -1.7 1,613,609 - RR42_RS27860 0.30 -0.3 1,613,774 + RR42_RS27860 0.47 +0.4 1,613,774 + RR42_RS27860 0.47 +0.7 1,613,862 - RR42_RS27860 0.56 +0.7
Or see this region's nucleotide sequence