Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27220 and RR42_RS27225 overlap by 14 nucleotides RR42_RS27225 and RR42_RS27230 are separated by 459 nucleotides RR42_RS27230 and RR42_RS27235 are separated by 4 nucleotides
RR42_RS27220: RR42_RS27220 - tricarballylate dehydrogenase, at 1,458,437 to 1,459,864
_RS27220
RR42_RS27225: RR42_RS27225 - tricarballylate utilization protein B, at 1,459,851 to 1,461,023
_RS27225
RR42_RS27230: RR42_RS27230 - DNA-binding protein, at 1,461,483 to 1,461,890
_RS27230
RR42_RS27235: RR42_RS27235 - acetyl-CoA acetyltransferase, at 1,461,895 to 1,463,061
_RS27235
Position (kb)
1459
1460
1461
1462 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1458.876 kb on - strand, within RR42_RS27220 at 1458.876 kb on - strand, within RR42_RS27220 at 1458.876 kb on - strand, within RR42_RS27220 at 1459.181 kb on + strand, within RR42_RS27220 at 1459.181 kb on + strand, within RR42_RS27220 at 1459.181 kb on + strand, within RR42_RS27220 at 1459.289 kb on + strand, within RR42_RS27220 at 1459.595 kb on + strand, within RR42_RS27220 at 1459.621 kb on + strand, within RR42_RS27220 at 1459.621 kb on + strand, within RR42_RS27220 at 1459.621 kb on + strand, within RR42_RS27220 at 1459.621 kb on + strand, within RR42_RS27220 at 1459.626 kb on - strand, within RR42_RS27220 at 1459.626 kb on - strand, within RR42_RS27220 at 1459.736 kb on + strand at 1459.737 kb on - strand at 1459.737 kb on - strand at 1459.738 kb on + strand at 1459.739 kb on - strand at 1459.739 kb on - strand at 1459.739 kb on - strand at 1459.739 kb on - strand at 1459.739 kb on - strand at 1460.040 kb on + strand, within RR42_RS27225 at 1460.041 kb on - strand, within RR42_RS27225 at 1460.041 kb on - strand, within RR42_RS27225 at 1460.041 kb on - strand, within RR42_RS27225 at 1460.076 kb on + strand, within RR42_RS27225 at 1460.158 kb on - strand, within RR42_RS27225 at 1460.158 kb on - strand, within RR42_RS27225 at 1460.167 kb on - strand, within RR42_RS27225 at 1460.493 kb on + strand, within RR42_RS27225 at 1460.494 kb on - strand, within RR42_RS27225 at 1460.581 kb on - strand, within RR42_RS27225 at 1460.631 kb on + strand, within RR42_RS27225 at 1460.631 kb on + strand, within RR42_RS27225 at 1460.632 kb on - strand, within RR42_RS27225 at 1460.632 kb on - strand, within RR42_RS27225 at 1460.926 kb on - strand at 1460.949 kb on + strand at 1461.283 kb on + strand at 1461.634 kb on + strand, within RR42_RS27230 at 1461.634 kb on + strand, within RR42_RS27230 at 1461.758 kb on - strand, within RR42_RS27230 at 1461.877 kb on + strand at 1461.878 kb on - strand at 1461.878 kb on - strand at 1461.878 kb on - strand at 1461.893 kb on + strand at 1461.894 kb on - strand at 1461.894 kb on - strand at 1461.896 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 1,458,876 - RR42_RS27220 0.31 -2.1 1,458,876 - RR42_RS27220 0.31 -0.2 1,458,876 - RR42_RS27220 0.31 -0.3 1,459,181 + RR42_RS27220 0.52 +0.6 1,459,181 + RR42_RS27220 0.52 -0.5 1,459,181 + RR42_RS27220 0.52 +0.3 1,459,289 + RR42_RS27220 0.60 -0.1 1,459,595 + RR42_RS27220 0.81 +0.3 1,459,621 + RR42_RS27220 0.83 +0.5 1,459,621 + RR42_RS27220 0.83 -0.8 1,459,621 + RR42_RS27220 0.83 +0.3 1,459,621 + RR42_RS27220 0.83 -0.8 1,459,626 - RR42_RS27220 0.83 -0.6 1,459,626 - RR42_RS27220 0.83 -0.2 1,459,736 + -0.5 1,459,737 - -1.2 1,459,737 - -2.0 1,459,738 + -1.2 1,459,739 - +0.3 1,459,739 - +1.2 1,459,739 - -0.7 1,459,739 - -0.2 1,459,739 - -0.4 1,460,040 + RR42_RS27225 0.16 -1.1 1,460,041 - RR42_RS27225 0.16 -1.7 1,460,041 - RR42_RS27225 0.16 +0.0 1,460,041 - RR42_RS27225 0.16 +0.4 1,460,076 + RR42_RS27225 0.19 -1.0 1,460,158 - RR42_RS27225 0.26 +2.2 1,460,158 - RR42_RS27225 0.26 +0.2 1,460,167 - RR42_RS27225 0.27 -0.6 1,460,493 + RR42_RS27225 0.55 -1.6 1,460,494 - RR42_RS27225 0.55 +0.4 1,460,581 - RR42_RS27225 0.62 -0.4 1,460,631 + RR42_RS27225 0.66 -0.8 1,460,631 + RR42_RS27225 0.66 -1.0 1,460,632 - RR42_RS27225 0.67 +0.2 1,460,632 - RR42_RS27225 0.67 -0.4 1,460,926 - -0.6 1,460,949 + +0.8 1,461,283 + -1.3 1,461,634 + RR42_RS27230 0.37 -0.2 1,461,634 + RR42_RS27230 0.37 -2.8 1,461,758 - RR42_RS27230 0.67 -3.2 1,461,877 + +0.1 1,461,878 - +0.5 1,461,878 - -0.8 1,461,878 - +0.8 1,461,893 + -0.6 1,461,894 - +0.6 1,461,894 - +0.0 1,461,896 + +0.8
Or see this region's nucleotide sequence