Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26255 and RR42_RS26260 are separated by 101 nucleotides RR42_RS26260 and RR42_RS26265 are separated by 485 nucleotides
RR42_RS26255: RR42_RS26255 - aldehyde dehydrogenase, at 1,248,972 to 1,250,471
_RS26255
RR42_RS26260: RR42_RS26260 - hypothetical protein, at 1,250,573 to 1,251,274
_RS26260
RR42_RS26265: RR42_RS26265 - hypothetical protein, at 1,251,760 to 1,251,990
_RS26265
Position (kb)
1250
1251
1252 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1249.737 kb on + strand, within RR42_RS26255 at 1249.737 kb on + strand, within RR42_RS26255 at 1249.737 kb on + strand, within RR42_RS26255 at 1249.738 kb on - strand, within RR42_RS26255 at 1249.797 kb on - strand, within RR42_RS26255 at 1249.899 kb on + strand, within RR42_RS26255 at 1249.899 kb on + strand, within RR42_RS26255 at 1249.899 kb on + strand, within RR42_RS26255 at 1249.899 kb on + strand, within RR42_RS26255 at 1249.900 kb on - strand, within RR42_RS26255 at 1249.900 kb on - strand, within RR42_RS26255 at 1249.917 kb on + strand, within RR42_RS26255 at 1249.918 kb on - strand, within RR42_RS26255 at 1250.040 kb on + strand, within RR42_RS26255 at 1250.040 kb on + strand, within RR42_RS26255 at 1250.041 kb on - strand, within RR42_RS26255 at 1250.118 kb on + strand, within RR42_RS26255 at 1250.118 kb on + strand, within RR42_RS26255 at 1250.118 kb on + strand, within RR42_RS26255 at 1250.119 kb on - strand, within RR42_RS26255 at 1250.346 kb on + strand at 1250.346 kb on + strand at 1250.346 kb on + strand at 1250.347 kb on - strand at 1250.347 kb on - strand at 1250.347 kb on - strand at 1250.348 kb on + strand at 1250.348 kb on + strand at 1250.348 kb on + strand at 1250.433 kb on + strand at 1250.434 kb on - strand at 1250.434 kb on - strand at 1250.597 kb on - strand at 1250.619 kb on + strand at 1250.619 kb on + strand at 1250.619 kb on + strand at 1250.658 kb on + strand, within RR42_RS26260 at 1250.658 kb on + strand, within RR42_RS26260 at 1250.658 kb on + strand, within RR42_RS26260 at 1250.658 kb on + strand, within RR42_RS26260 at 1250.658 kb on + strand, within RR42_RS26260 at 1250.658 kb on + strand, within RR42_RS26260 at 1250.659 kb on - strand, within RR42_RS26260 at 1250.699 kb on - strand, within RR42_RS26260 at 1250.699 kb on - strand, within RR42_RS26260 at 1250.699 kb on - strand at 1250.714 kb on + strand, within RR42_RS26260 at 1250.748 kb on - strand, within RR42_RS26260 at 1250.748 kb on - strand, within RR42_RS26260 at 1250.760 kb on + strand, within RR42_RS26260 at 1250.761 kb on - strand, within RR42_RS26260 at 1250.761 kb on - strand, within RR42_RS26260 at 1251.063 kb on - strand, within RR42_RS26260 at 1251.063 kb on - strand, within RR42_RS26260 at 1251.208 kb on + strand at 1251.279 kb on + strand at 1251.279 kb on + strand at 1251.280 kb on - strand at 1251.301 kb on + strand at 1251.448 kb on - strand at 1251.462 kb on + strand at 1251.463 kb on - strand at 1251.463 kb on - strand at 1251.488 kb on + strand at 1251.491 kb on + strand at 1251.491 kb on + strand at 1251.491 kb on + strand at 1251.491 kb on + strand at 1251.492 kb on - strand at 1251.518 kb on + strand at 1251.518 kb on + strand at 1251.519 kb on - strand at 1251.760 kb on + strand at 1251.761 kb on - strand at 1251.761 kb on - strand at 1251.824 kb on + strand, within RR42_RS26265 at 1252.253 kb on + strand at 1252.253 kb on + strand at 1252.253 kb on + strand at 1252.253 kb on + strand at 1252.253 kb on + strand at 1252.254 kb on - strand at 1252.254 kb on - strand at 1252.254 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 1,249,737 + RR42_RS26255 0.51 -0.7 1,249,737 + RR42_RS26255 0.51 -0.2 1,249,737 + RR42_RS26255 0.51 -0.9 1,249,738 - RR42_RS26255 0.51 -0.3 1,249,797 - RR42_RS26255 0.55 +0.0 1,249,899 + RR42_RS26255 0.62 +0.8 1,249,899 + RR42_RS26255 0.62 +0.0 1,249,899 + RR42_RS26255 0.62 +1.3 1,249,899 + RR42_RS26255 0.62 +0.2 1,249,900 - RR42_RS26255 0.62 +0.7 1,249,900 - RR42_RS26255 0.62 -0.6 1,249,917 + RR42_RS26255 0.63 +0.4 1,249,918 - RR42_RS26255 0.63 +0.2 1,250,040 + RR42_RS26255 0.71 -0.4 1,250,040 + RR42_RS26255 0.71 -1.3 1,250,041 - RR42_RS26255 0.71 +0.3 1,250,118 + RR42_RS26255 0.76 +0.8 1,250,118 + RR42_RS26255 0.76 +0.1 1,250,118 + RR42_RS26255 0.76 +0.6 1,250,119 - RR42_RS26255 0.76 -0.3 1,250,346 + -1.5 1,250,346 + -0.6 1,250,346 + +0.0 1,250,347 - -0.1 1,250,347 - -0.1 1,250,347 - -0.3 1,250,348 + +1.6 1,250,348 + -1.3 1,250,348 + -0.3 1,250,433 + +1.9 1,250,434 - -1.4 1,250,434 - -0.9 1,250,597 - +0.2 1,250,619 + -0.1 1,250,619 + -0.5 1,250,619 + -0.8 1,250,658 + RR42_RS26260 0.12 +0.4 1,250,658 + RR42_RS26260 0.12 -1.1 1,250,658 + RR42_RS26260 0.12 +0.9 1,250,658 + RR42_RS26260 0.12 +0.3 1,250,658 + RR42_RS26260 0.12 -1.9 1,250,658 + RR42_RS26260 0.12 +0.6 1,250,659 - RR42_RS26260 0.12 +0.7 1,250,699 - RR42_RS26260 0.18 +0.9 1,250,699 - RR42_RS26260 0.18 +0.4 1,250,699 - -0.1 1,250,714 + RR42_RS26260 0.20 -0.6 1,250,748 - RR42_RS26260 0.25 +0.5 1,250,748 - RR42_RS26260 0.25 +0.2 1,250,760 + RR42_RS26260 0.27 -1.9 1,250,761 - RR42_RS26260 0.27 -0.2 1,250,761 - RR42_RS26260 0.27 -0.9 1,251,063 - RR42_RS26260 0.70 +0.9 1,251,063 - RR42_RS26260 0.70 -0.6 1,251,208 + +0.6 1,251,279 + -1.7 1,251,279 + -2.2 1,251,280 - -1.9 1,251,301 + -0.4 1,251,448 - -0.1 1,251,462 + +0.9 1,251,463 - -1.4 1,251,463 - -0.7 1,251,488 + +1.0 1,251,491 + +0.3 1,251,491 + +0.1 1,251,491 + -0.8 1,251,491 + -0.9 1,251,492 - -0.5 1,251,518 + +0.0 1,251,518 + -0.7 1,251,519 - +0.7 1,251,760 + +0.4 1,251,761 - +1.1 1,251,761 - -0.1 1,251,824 + RR42_RS26265 0.28 -0.4 1,252,253 + -1.1 1,252,253 + +2.1 1,252,253 + +0.6 1,252,253 + +0.9 1,252,253 + -1.8 1,252,254 - +0.6 1,252,254 - -1.6 1,252,254 - -0.4
Or see this region's nucleotide sequence