Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25535 and RR42_RS25540 are separated by 419 nucleotides RR42_RS25540 and RR42_RS25545 are separated by 8 nucleotides
RR42_RS25535: RR42_RS25535 - TetR family transcriptional regulator, at 1,074,223 to 1,074,834
_RS25535
RR42_RS25540: RR42_RS25540 - hypothetical protein, at 1,075,254 to 1,075,718
_RS25540
RR42_RS25545: RR42_RS25545 - NADPH:quinone oxidoreductase, at 1,075,727 to 1,076,728
_RS25545
Position (kb)
1075
1076 Strain fitness (log2 ratio)
-2
-1
0
1 at 1074.332 kb on - strand, within RR42_RS25535 at 1074.392 kb on - strand, within RR42_RS25535 at 1074.526 kb on + strand, within RR42_RS25535 at 1074.526 kb on + strand, within RR42_RS25535 at 1074.527 kb on - strand, within RR42_RS25535 at 1074.527 kb on - strand, within RR42_RS25535 at 1074.581 kb on + strand, within RR42_RS25535 at 1074.582 kb on - strand, within RR42_RS25535 at 1074.656 kb on + strand, within RR42_RS25535 at 1074.656 kb on + strand, within RR42_RS25535 at 1074.657 kb on - strand, within RR42_RS25535 at 1074.657 kb on - strand, within RR42_RS25535 at 1074.724 kb on - strand, within RR42_RS25535 at 1074.724 kb on - strand, within RR42_RS25535 at 1075.015 kb on - strand at 1075.081 kb on + strand at 1075.081 kb on + strand at 1075.081 kb on + strand at 1075.081 kb on + strand at 1075.081 kb on + strand at 1075.082 kb on - strand at 1075.092 kb on + strand at 1075.093 kb on - strand at 1075.093 kb on - strand at 1075.145 kb on + strand at 1075.146 kb on - strand at 1075.146 kb on - strand at 1075.146 kb on - strand at 1075.146 kb on - strand at 1075.150 kb on - strand at 1075.150 kb on - strand at 1075.150 kb on - strand at 1075.441 kb on + strand, within RR42_RS25540 at 1075.442 kb on - strand, within RR42_RS25540 at 1075.569 kb on + strand, within RR42_RS25540 at 1075.570 kb on - strand, within RR42_RS25540 at 1075.570 kb on - strand, within RR42_RS25540 at 1075.570 kb on - strand, within RR42_RS25540 at 1075.570 kb on - strand, within RR42_RS25540 at 1075.585 kb on + strand, within RR42_RS25540 at 1075.585 kb on + strand, within RR42_RS25540 at 1075.666 kb on - strand, within RR42_RS25540 at 1075.988 kb on + strand, within RR42_RS25545 at 1075.988 kb on + strand, within RR42_RS25545 at 1075.989 kb on - strand, within RR42_RS25545 at 1075.989 kb on - strand, within RR42_RS25545 at 1076.031 kb on - strand, within RR42_RS25545 at 1076.170 kb on - strand, within RR42_RS25545 at 1076.196 kb on + strand, within RR42_RS25545 at 1076.196 kb on + strand, within RR42_RS25545 at 1076.241 kb on + strand, within RR42_RS25545 at 1076.242 kb on - strand, within RR42_RS25545 at 1076.278 kb on - strand, within RR42_RS25545 at 1076.310 kb on - strand, within RR42_RS25545 at 1076.335 kb on - strand, within RR42_RS25545 at 1076.345 kb on + strand, within RR42_RS25545 at 1076.510 kb on + strand, within RR42_RS25545 at 1076.511 kb on - strand, within RR42_RS25545 at 1076.532 kb on + strand, within RR42_RS25545 at 1076.532 kb on + strand, within RR42_RS25545 at 1076.533 kb on - strand, within RR42_RS25545 at 1076.678 kb on + strand at 1076.679 kb on - strand at 1076.679 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 1,074,332 - RR42_RS25535 0.18 -1.3 1,074,392 - RR42_RS25535 0.28 -1.5 1,074,526 + RR42_RS25535 0.50 +0.6 1,074,526 + RR42_RS25535 0.50 -1.1 1,074,527 - RR42_RS25535 0.50 +0.1 1,074,527 - RR42_RS25535 0.50 -1.3 1,074,581 + RR42_RS25535 0.58 +0.9 1,074,582 - RR42_RS25535 0.59 -2.2 1,074,656 + RR42_RS25535 0.71 -0.3 1,074,656 + RR42_RS25535 0.71 -0.9 1,074,657 - RR42_RS25535 0.71 -0.7 1,074,657 - RR42_RS25535 0.71 -0.0 1,074,724 - RR42_RS25535 0.82 +1.1 1,074,724 - RR42_RS25535 0.82 -0.1 1,075,015 - -0.0 1,075,081 + +0.3 1,075,081 + +0.4 1,075,081 + -1.1 1,075,081 + -0.7 1,075,081 + -0.6 1,075,082 - -0.4 1,075,092 + +1.5 1,075,093 - -0.4 1,075,093 - -1.8 1,075,145 + -0.8 1,075,146 - -1.0 1,075,146 - -0.7 1,075,146 - +1.4 1,075,146 - +0.2 1,075,150 - -0.0 1,075,150 - +0.1 1,075,150 - +0.3 1,075,441 + RR42_RS25540 0.40 -0.1 1,075,442 - RR42_RS25540 0.40 -0.9 1,075,569 + RR42_RS25540 0.68 -0.0 1,075,570 - RR42_RS25540 0.68 +0.1 1,075,570 - RR42_RS25540 0.68 +0.0 1,075,570 - RR42_RS25540 0.68 +0.5 1,075,570 - RR42_RS25540 0.68 +1.3 1,075,585 + RR42_RS25540 0.71 -2.4 1,075,585 + RR42_RS25540 0.71 -0.0 1,075,666 - RR42_RS25540 0.89 +0.1 1,075,988 + RR42_RS25545 0.26 +0.9 1,075,988 + RR42_RS25545 0.26 -1.3 1,075,989 - RR42_RS25545 0.26 -0.7 1,075,989 - RR42_RS25545 0.26 -1.0 1,076,031 - RR42_RS25545 0.30 +0.2 1,076,170 - RR42_RS25545 0.44 +0.3 1,076,196 + RR42_RS25545 0.47 -0.5 1,076,196 + RR42_RS25545 0.47 +0.0 1,076,241 + RR42_RS25545 0.51 +0.5 1,076,242 - RR42_RS25545 0.51 +0.5 1,076,278 - RR42_RS25545 0.55 +0.5 1,076,310 - RR42_RS25545 0.58 +1.3 1,076,335 - RR42_RS25545 0.61 +0.1 1,076,345 + RR42_RS25545 0.62 -0.7 1,076,510 + RR42_RS25545 0.78 +1.1 1,076,511 - RR42_RS25545 0.78 -1.7 1,076,532 + RR42_RS25545 0.80 -0.3 1,076,532 + RR42_RS25545 0.80 +0.9 1,076,533 - RR42_RS25545 0.80 -0.8 1,076,678 + -0.8 1,076,679 - -1.1 1,076,679 - +1.6
Or see this region's nucleotide sequence