Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23310 and RR42_RS23315 overlap by 4 nucleotides RR42_RS23315 and RR42_RS23320 are separated by 84 nucleotides
RR42_RS23310: RR42_RS23310 - cytochrome B6, at 559,526 to 560,863
_RS23310
RR42_RS23315: RR42_RS23315 - serine/threonine protein phosphatase, at 560,860 to 562,416
_RS23315
RR42_RS23320: RR42_RS23320 - hypothetical protein, at 562,501 to 564,165
_RS23320
Position (kb) 560
561
562
563 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 560.127 kb on + strand, within RR42_RS23310 at 560.176 kb on - strand, within RR42_RS23310 at 560.406 kb on + strand, within RR42_RS23310 at 560.428 kb on - strand, within RR42_RS23310 at 560.428 kb on - strand, within RR42_RS23310 at 560.448 kb on + strand, within RR42_RS23310 at 560.449 kb on - strand, within RR42_RS23310 at 560.449 kb on - strand, within RR42_RS23310 at 560.457 kb on + strand, within RR42_RS23310 at 560.457 kb on + strand, within RR42_RS23310 at 560.457 kb on + strand, within RR42_RS23310 at 560.458 kb on - strand, within RR42_RS23310 at 560.899 kb on - strand at 560.944 kb on - strand at 560.945 kb on + strand at 560.945 kb on + strand at 560.945 kb on + strand at 561.085 kb on + strand, within RR42_RS23315 at 561.207 kb on - strand, within RR42_RS23315 at 561.356 kb on - strand, within RR42_RS23315 at 561.488 kb on + strand, within RR42_RS23315 at 561.489 kb on - strand, within RR42_RS23315 at 561.843 kb on - strand, within RR42_RS23315 at 562.155 kb on - strand, within RR42_RS23315 at 562.420 kb on + strand at 562.420 kb on + strand at 562.420 kb on + strand at 562.420 kb on + strand at 562.420 kb on + strand at 562.420 kb on + strand at 562.420 kb on + strand at 562.420 kb on + strand at 562.420 kb on + strand at 562.421 kb on - strand at 562.421 kb on - strand at 562.421 kb on - strand at 562.421 kb on - strand at 562.421 kb on - strand at 562.422 kb on + strand at 562.422 kb on + strand at 562.422 kb on + strand at 562.422 kb on + strand at 562.423 kb on - strand at 562.423 kb on - strand at 562.423 kb on - strand at 562.433 kb on + strand at 562.433 kb on + strand at 562.456 kb on + strand at 562.459 kb on - strand at 562.459 kb on - strand at 562.489 kb on + strand at 562.489 kb on + strand at 562.489 kb on + strand at 562.490 kb on - strand at 562.490 kb on - strand at 562.490 kb on - strand at 562.754 kb on + strand, within RR42_RS23320 at 562.754 kb on + strand, within RR42_RS23320 at 563.168 kb on + strand, within RR42_RS23320 at 563.168 kb on + strand, within RR42_RS23320 at 563.169 kb on - strand, within RR42_RS23320 at 563.169 kb on - strand, within RR42_RS23320 at 563.169 kb on - strand, within RR42_RS23320 at 563.319 kb on - strand, within RR42_RS23320
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 560,127 + RR42_RS23310 0.45 -1.8 560,176 - RR42_RS23310 0.49 -0.2 560,406 + RR42_RS23310 0.66 -1.1 560,428 - RR42_RS23310 0.67 +0.1 560,428 - RR42_RS23310 0.67 -0.0 560,448 + RR42_RS23310 0.69 +1.7 560,449 - RR42_RS23310 0.69 +0.4 560,449 - RR42_RS23310 0.69 +0.1 560,457 + RR42_RS23310 0.70 +0.9 560,457 + RR42_RS23310 0.70 -0.5 560,457 + RR42_RS23310 0.70 -3.0 560,458 - RR42_RS23310 0.70 -1.3 560,899 - +0.2 560,944 - -2.1 560,945 + -3.1 560,945 + +0.3 560,945 + -0.8 561,085 + RR42_RS23315 0.14 +1.0 561,207 - RR42_RS23315 0.22 -0.7 561,356 - RR42_RS23315 0.32 -1.6 561,488 + RR42_RS23315 0.40 +0.7 561,489 - RR42_RS23315 0.40 -0.6 561,843 - RR42_RS23315 0.63 -0.4 562,155 - RR42_RS23315 0.83 -0.3 562,420 + +0.4 562,420 + +0.8 562,420 + +0.5 562,420 + +1.5 562,420 + -1.8 562,420 + -1.3 562,420 + -0.1 562,420 + -0.7 562,420 + +0.1 562,421 - -0.8 562,421 - -2.9 562,421 - -1.3 562,421 - -0.6 562,421 - -0.3 562,422 + -0.1 562,422 + +1.1 562,422 + -0.1 562,422 + -1.2 562,423 - +0.7 562,423 - -0.6 562,423 - -0.6 562,433 + +1.3 562,433 + +0.7 562,456 + -0.5 562,459 - +0.2 562,459 - +0.6 562,489 + +2.0 562,489 + +2.6 562,489 + -0.3 562,490 - +1.5 562,490 - -1.0 562,490 - +0.9 562,754 + RR42_RS23320 0.15 +0.7 562,754 + RR42_RS23320 0.15 +1.6 563,168 + RR42_RS23320 0.40 -0.5 563,168 + RR42_RS23320 0.40 +0.9 563,169 - RR42_RS23320 0.40 +1.1 563,169 - RR42_RS23320 0.40 -0.6 563,169 - RR42_RS23320 0.40 +0.9 563,319 - RR42_RS23320 0.49 -1.1
Or see this region's nucleotide sequence