Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23090 and RR42_RS23095 are separated by 92 nucleotides RR42_RS23095 and RR42_RS23100 are separated by 89 nucleotides RR42_RS23100 and RR42_RS23105 are separated by 135 nucleotides RR42_RS23105 and RR42_RS23110 are separated by 193 nucleotides
RR42_RS23090: RR42_RS23090 - 2,5-dioxovalerate dehydrogenase, at 514,711 to 516,312
_RS23090
RR42_RS23095: RR42_RS23095 - Zn-dependent hydrolase, at 516,405 to 517,169
_RS23095
RR42_RS23100: RR42_RS23100 - AraC family transcriptional regulator, at 517,259 to 518,224
_RS23100
RR42_RS23105: RR42_RS23105 - hypothetical protein, at 518,360 to 518,818
_RS23105
RR42_RS23110: RR42_RS23110 - isochorismatase, at 519,012 to 519,542
_RS23110
Position (kb) 517
518
519 Strain fitness (log2 ratio) -2
-1
0
1 at 516.304 kb on - strand at 516.428 kb on + strand at 516.428 kb on + strand at 516.675 kb on + strand, within RR42_RS23095 at 516.695 kb on - strand, within RR42_RS23095 at 516.695 kb on - strand, within RR42_RS23095 at 516.715 kb on - strand, within RR42_RS23095 at 516.715 kb on - strand, within RR42_RS23095 at 516.872 kb on - strand, within RR42_RS23095 at 517.163 kb on + strand at 517.163 kb on + strand at 517.163 kb on + strand at 517.163 kb on + strand at 517.164 kb on - strand at 517.164 kb on - strand at 517.164 kb on - strand at 517.515 kb on + strand, within RR42_RS23100 at 517.584 kb on + strand, within RR42_RS23100 at 517.592 kb on + strand, within RR42_RS23100 at 517.592 kb on + strand, within RR42_RS23100 at 517.592 kb on + strand, within RR42_RS23100 at 517.593 kb on - strand, within RR42_RS23100 at 517.593 kb on - strand, within RR42_RS23100 at 517.593 kb on - strand, within RR42_RS23100 at 517.688 kb on + strand, within RR42_RS23100 at 517.688 kb on + strand, within RR42_RS23100 at 517.689 kb on - strand, within RR42_RS23100 at 517.738 kb on + strand, within RR42_RS23100 at 518.052 kb on - strand, within RR42_RS23100 at 518.052 kb on - strand, within RR42_RS23100 at 518.052 kb on - strand, within RR42_RS23100 at 518.092 kb on + strand, within RR42_RS23100 at 518.118 kb on + strand, within RR42_RS23100 at 518.118 kb on + strand, within RR42_RS23100 at 518.119 kb on - strand, within RR42_RS23100 at 518.119 kb on - strand, within RR42_RS23100 at 518.119 kb on - strand, within RR42_RS23100 at 518.155 kb on + strand at 518.155 kb on + strand at 518.202 kb on - strand at 518.250 kb on + strand at 518.251 kb on - strand at 518.419 kb on - strand, within RR42_RS23105 at 518.419 kb on - strand, within RR42_RS23105 at 518.608 kb on + strand, within RR42_RS23105 at 518.608 kb on + strand, within RR42_RS23105 at 518.665 kb on - strand, within RR42_RS23105 at 518.665 kb on - strand, within RR42_RS23105 at 518.671 kb on - strand, within RR42_RS23105 at 518.671 kb on - strand, within RR42_RS23105 at 518.671 kb on - strand, within RR42_RS23105 at 518.704 kb on - strand, within RR42_RS23105 at 518.918 kb on + strand at 518.918 kb on + strand at 518.918 kb on + strand at 518.982 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 516,304 - -0.8 516,428 + +0.5 516,428 + -0.2 516,675 + RR42_RS23095 0.35 +0.2 516,695 - RR42_RS23095 0.38 -0.7 516,695 - RR42_RS23095 0.38 -0.8 516,715 - RR42_RS23095 0.41 -1.6 516,715 - RR42_RS23095 0.41 -1.5 516,872 - RR42_RS23095 0.61 -1.3 517,163 + +1.0 517,163 + +0.0 517,163 + +0.3 517,163 + -0.5 517,164 - -0.0 517,164 - +0.2 517,164 - -1.3 517,515 + RR42_RS23100 0.27 +0.4 517,584 + RR42_RS23100 0.34 +0.2 517,592 + RR42_RS23100 0.34 +0.5 517,592 + RR42_RS23100 0.34 -0.8 517,592 + RR42_RS23100 0.34 -0.1 517,593 - RR42_RS23100 0.35 +0.5 517,593 - RR42_RS23100 0.35 +0.0 517,593 - RR42_RS23100 0.35 +0.2 517,688 + RR42_RS23100 0.44 -1.8 517,688 + RR42_RS23100 0.44 -0.4 517,689 - RR42_RS23100 0.45 +0.3 517,738 + RR42_RS23100 0.50 +0.3 518,052 - RR42_RS23100 0.82 -0.0 518,052 - RR42_RS23100 0.82 -2.6 518,052 - RR42_RS23100 0.82 -0.5 518,092 + RR42_RS23100 0.86 -0.9 518,118 + RR42_RS23100 0.89 +0.0 518,118 + RR42_RS23100 0.89 -0.3 518,119 - RR42_RS23100 0.89 +0.6 518,119 - RR42_RS23100 0.89 -0.1 518,119 - RR42_RS23100 0.89 +0.1 518,155 + -1.0 518,155 + -0.1 518,202 - -0.3 518,250 + +0.6 518,251 - +0.5 518,419 - RR42_RS23105 0.13 -1.2 518,419 - RR42_RS23105 0.13 +1.4 518,608 + RR42_RS23105 0.54 -0.0 518,608 + RR42_RS23105 0.54 +0.4 518,665 - RR42_RS23105 0.66 -0.9 518,665 - RR42_RS23105 0.66 -2.1 518,671 - RR42_RS23105 0.68 -1.3 518,671 - RR42_RS23105 0.68 +0.7 518,671 - RR42_RS23105 0.68 -0.4 518,704 - RR42_RS23105 0.75 -0.7 518,918 + -0.4 518,918 + +1.3 518,918 + -0.9 518,982 - +1.0
Or see this region's nucleotide sequence