Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19660 and RR42_RS19665 are separated by 78 nucleotides RR42_RS19665 and RR42_RS19670 are separated by 36 nucleotides RR42_RS19670 and RR42_RS19675 are separated by 50 nucleotides
RR42_RS19660: RR42_RS19660 - 5'-nucleotidase, at 4,248,726 to 4,250,447
_RS19660
RR42_RS19665: RR42_RS19665 - alkyl hydroperoxide reductase, at 4,250,526 to 4,251,023
_RS19665
RR42_RS19670: RR42_RS19670 - SoxX protein, at 4,251,060 to 4,251,719
_RS19670
RR42_RS19675: RR42_RS19675 - SoxA protein, at 4,251,770 to 4,252,600
_RS19675
Position (kb) 4251
4252 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4250.143 kb on + strand, within RR42_RS19660 at 4250.143 kb on + strand, within RR42_RS19660 at 4250.143 kb on + strand, within RR42_RS19660 at 4250.345 kb on - strand at 4250.527 kb on + strand at 4250.527 kb on + strand at 4250.629 kb on + strand, within RR42_RS19665 at 4250.629 kb on + strand, within RR42_RS19665 at 4250.630 kb on - strand, within RR42_RS19665 at 4250.630 kb on - strand, within RR42_RS19665 at 4250.704 kb on + strand, within RR42_RS19665 at 4250.704 kb on + strand, within RR42_RS19665 at 4250.704 kb on + strand, within RR42_RS19665 at 4250.705 kb on - strand, within RR42_RS19665 at 4250.722 kb on + strand, within RR42_RS19665 at 4250.722 kb on + strand, within RR42_RS19665 at 4250.722 kb on + strand, within RR42_RS19665 at 4250.723 kb on - strand, within RR42_RS19665 at 4250.723 kb on - strand, within RR42_RS19665 at 4250.952 kb on + strand, within RR42_RS19665 at 4251.205 kb on + strand, within RR42_RS19670 at 4251.206 kb on - strand, within RR42_RS19670 at 4251.250 kb on + strand, within RR42_RS19670 at 4251.251 kb on - strand, within RR42_RS19670 at 4251.251 kb on - strand, within RR42_RS19670 at 4251.310 kb on + strand, within RR42_RS19670 at 4251.310 kb on + strand, within RR42_RS19670 at 4251.310 kb on + strand, within RR42_RS19670 at 4251.311 kb on - strand, within RR42_RS19670 at 4251.311 kb on - strand, within RR42_RS19670 at 4251.311 kb on - strand, within RR42_RS19670 at 4251.311 kb on - strand, within RR42_RS19670 at 4251.566 kb on + strand, within RR42_RS19670 at 4251.734 kb on - strand at 4251.734 kb on - strand at 4251.795 kb on + strand at 4251.795 kb on + strand at 4251.796 kb on - strand at 4251.796 kb on - strand at 4251.796 kb on - strand at 4251.796 kb on - strand at 4251.858 kb on + strand, within RR42_RS19675 at 4251.897 kb on + strand, within RR42_RS19675 at 4251.898 kb on - strand, within RR42_RS19675 at 4251.920 kb on + strand, within RR42_RS19675 at 4251.920 kb on + strand, within RR42_RS19675 at 4251.961 kb on - strand, within RR42_RS19675 at 4251.961 kb on - strand, within RR42_RS19675 at 4251.961 kb on - strand, within RR42_RS19675 at 4252.060 kb on - strand, within RR42_RS19675 at 4252.102 kb on - strand, within RR42_RS19675 at 4252.269 kb on + strand, within RR42_RS19675 at 4252.664 kb on + strand at 4252.664 kb on + strand at 4252.664 kb on + strand at 4252.665 kb on - strand at 4252.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 4,250,143 + RR42_RS19660 0.82 +0.4 4,250,143 + RR42_RS19660 0.82 +1.2 4,250,143 + RR42_RS19660 0.82 +0.3 4,250,345 - +0.9 4,250,527 + +0.9 4,250,527 + +0.9 4,250,629 + RR42_RS19665 0.21 +0.8 4,250,629 + RR42_RS19665 0.21 +0.4 4,250,630 - RR42_RS19665 0.21 +1.4 4,250,630 - RR42_RS19665 0.21 +1.3 4,250,704 + RR42_RS19665 0.36 -0.1 4,250,704 + RR42_RS19665 0.36 -0.2 4,250,704 + RR42_RS19665 0.36 -0.2 4,250,705 - RR42_RS19665 0.36 -0.3 4,250,722 + RR42_RS19665 0.39 +0.3 4,250,722 + RR42_RS19665 0.39 -0.3 4,250,722 + RR42_RS19665 0.39 -2.8 4,250,723 - RR42_RS19665 0.40 +0.8 4,250,723 - RR42_RS19665 0.40 -0.8 4,250,952 + RR42_RS19665 0.86 +0.9 4,251,205 + RR42_RS19670 0.22 -0.6 4,251,206 - RR42_RS19670 0.22 +0.7 4,251,250 + RR42_RS19670 0.29 +1.5 4,251,251 - RR42_RS19670 0.29 +1.0 4,251,251 - RR42_RS19670 0.29 -0.3 4,251,310 + RR42_RS19670 0.38 +0.6 4,251,310 + RR42_RS19670 0.38 +0.3 4,251,310 + RR42_RS19670 0.38 +1.3 4,251,311 - RR42_RS19670 0.38 -0.2 4,251,311 - RR42_RS19670 0.38 -0.7 4,251,311 - RR42_RS19670 0.38 +1.3 4,251,311 - RR42_RS19670 0.38 -0.4 4,251,566 + RR42_RS19670 0.77 +0.4 4,251,734 - -2.5 4,251,734 - +1.0 4,251,795 + +1.0 4,251,795 + +0.9 4,251,796 - -0.5 4,251,796 - -0.3 4,251,796 - +1.1 4,251,796 - +1.1 4,251,858 + RR42_RS19675 0.11 +0.3 4,251,897 + RR42_RS19675 0.15 +1.1 4,251,898 - RR42_RS19675 0.15 +0.1 4,251,920 + RR42_RS19675 0.18 +1.8 4,251,920 + RR42_RS19675 0.18 +1.4 4,251,961 - RR42_RS19675 0.23 -0.3 4,251,961 - RR42_RS19675 0.23 +0.1 4,251,961 - RR42_RS19675 0.23 +1.2 4,252,060 - RR42_RS19675 0.35 +0.6 4,252,102 - RR42_RS19675 0.40 +0.5 4,252,269 + RR42_RS19675 0.60 +1.7 4,252,664 + +0.1 4,252,664 + +0.6 4,252,664 + -0.1 4,252,665 - -0.4 4,252,665 - -0.7
Or see this region's nucleotide sequence