Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS15540

Experiment: R2A with Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS15530 and RR42_RS15535 are separated by 74 nucleotidesRR42_RS15535 and RR42_RS15540 are separated by 95 nucleotidesRR42_RS15540 and RR42_RS15545 are separated by 64 nucleotidesRR42_RS15545 and RR42_RS15550 are separated by 7 nucleotides RR42_RS15530: RR42_RS15530 - membrane protein, at 3,355,263 to 3,356,195 _RS15530 RR42_RS15535: RR42_RS15535 - hypothetical protein, at 3,356,270 to 3,356,818 _RS15535 RR42_RS15540: RR42_RS15540 - hypothetical protein, at 3,356,914 to 3,358,347 _RS15540 RR42_RS15545: RR42_RS15545 - pilus assembly protein PilE, at 3,358,412 to 3,358,861 _RS15545 RR42_RS15550: RR42_RS15550 - pilus assembly protein, at 3,358,869 to 3,359,441 _RS15550 Position (kb) 3356 3357 3358 3359Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3355.970 kb on - strand, within RR42_RS15530at 3355.970 kb on - strand, within RR42_RS15530at 3356.042 kb on + strand, within RR42_RS15530at 3356.043 kb on - strand, within RR42_RS15530at 3356.043 kb on - strand, within RR42_RS15530at 3356.165 kb on + strandat 3356.188 kb on + strandat 3356.188 kb on + strandat 3356.188 kb on + strandat 3356.188 kb on + strandat 3356.188 kb on + strandat 3356.188 kb on + strandat 3356.188 kb on + strandat 3356.188 kb on + strandat 3356.189 kb on - strandat 3356.189 kb on - strandat 3356.189 kb on - strandat 3356.342 kb on + strand, within RR42_RS15535at 3356.342 kb on + strand, within RR42_RS15535at 3356.443 kb on - strand, within RR42_RS15535at 3356.446 kb on + strand, within RR42_RS15535at 3356.661 kb on - strand, within RR42_RS15535at 3356.715 kb on - strand, within RR42_RS15535at 3356.715 kb on - strand, within RR42_RS15535at 3356.796 kb on + strandat 3356.796 kb on + strandat 3356.797 kb on - strandat 3356.797 kb on - strandat 3357.057 kb on - strandat 3357.249 kb on - strand, within RR42_RS15540at 3357.350 kb on - strand, within RR42_RS15540at 3357.577 kb on - strand, within RR42_RS15540at 3357.601 kb on + strand, within RR42_RS15540at 3357.602 kb on - strand, within RR42_RS15540at 3357.691 kb on + strand, within RR42_RS15540at 3357.691 kb on + strand, within RR42_RS15540at 3357.692 kb on - strand, within RR42_RS15540at 3357.835 kb on - strand, within RR42_RS15540at 3357.858 kb on + strand, within RR42_RS15540at 3357.859 kb on - strand, within RR42_RS15540at 3357.952 kb on + strand, within RR42_RS15540at 3357.953 kb on - strand, within RR42_RS15540at 3357.953 kb on - strand, within RR42_RS15540at 3358.057 kb on + strand, within RR42_RS15540at 3358.124 kb on + strand, within RR42_RS15540at 3358.124 kb on + strand, within RR42_RS15540at 3358.125 kb on - strand, within RR42_RS15540at 3358.125 kb on - strand, within RR42_RS15540at 3358.125 kb on - strand, within RR42_RS15540at 3358.181 kb on + strand, within RR42_RS15540at 3358.181 kb on + strand, within RR42_RS15540at 3358.385 kb on + strandat 3358.385 kb on + strandat 3358.385 kb on + strandat 3358.386 kb on - strandat 3358.386 kb on - strandat 3358.487 kb on - strand, within RR42_RS15545at 3358.500 kb on + strand, within RR42_RS15545at 3358.501 kb on - strand, within RR42_RS15545at 3358.501 kb on - strand, within RR42_RS15545at 3358.519 kb on + strand, within RR42_RS15545at 3358.520 kb on - strand, within RR42_RS15545at 3358.520 kb on - strand, within RR42_RS15545at 3358.520 kb on - strand, within RR42_RS15545at 3358.520 kb on - strand, within RR42_RS15545at 3359.093 kb on - strand, within RR42_RS15550at 3359.255 kb on + strand, within RR42_RS15550at 3359.255 kb on + strand, within RR42_RS15550at 3359.255 kb on + strand, within RR42_RS15550at 3359.256 kb on - strand, within RR42_RS15550at 3359.256 kb on - strand, within RR42_RS15550at 3359.256 kb on - strand, within RR42_RS15550

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM
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3,355,970 - RR42_RS15530 0.76 +1.1
3,355,970 - RR42_RS15530 0.76 -1.7
3,356,042 + RR42_RS15530 0.83 -1.5
3,356,043 - RR42_RS15530 0.84 +1.8
3,356,043 - RR42_RS15530 0.84 -0.1
3,356,165 + +0.3
3,356,188 + -0.3
3,356,188 + -0.9
3,356,188 + +0.5
3,356,188 + +0.8
3,356,188 + +4.4
3,356,188 + +0.2
3,356,188 + -0.6
3,356,188 + -0.4
3,356,189 - +0.1
3,356,189 - -2.3
3,356,189 - +1.4
3,356,342 + RR42_RS15535 0.13 -2.7
3,356,342 + RR42_RS15535 0.13 -0.1
3,356,443 - RR42_RS15535 0.32 +0.0
3,356,446 + RR42_RS15535 0.32 -0.7
3,356,661 - RR42_RS15535 0.71 +1.1
3,356,715 - RR42_RS15535 0.81 -1.3
3,356,715 - RR42_RS15535 0.81 +0.3
3,356,796 + +0.7
3,356,796 + +0.7
3,356,797 - -0.4
3,356,797 - -0.4
3,357,057 - +1.0
3,357,249 - RR42_RS15540 0.23 +0.9
3,357,350 - RR42_RS15540 0.30 -0.4
3,357,577 - RR42_RS15540 0.46 +0.4
3,357,601 + RR42_RS15540 0.48 +1.1
3,357,602 - RR42_RS15540 0.48 -0.2
3,357,691 + RR42_RS15540 0.54 -1.1
3,357,691 + RR42_RS15540 0.54 -0.2
3,357,692 - RR42_RS15540 0.54 -0.3
3,357,835 - RR42_RS15540 0.64 +0.5
3,357,858 + RR42_RS15540 0.66 +0.8
3,357,859 - RR42_RS15540 0.66 -1.5
3,357,952 + RR42_RS15540 0.72 -0.5
3,357,953 - RR42_RS15540 0.72 -1.7
3,357,953 - RR42_RS15540 0.72 -0.4
3,358,057 + RR42_RS15540 0.80 +0.3
3,358,124 + RR42_RS15540 0.84 +0.9
3,358,124 + RR42_RS15540 0.84 +0.8
3,358,125 - RR42_RS15540 0.84 +1.0
3,358,125 - RR42_RS15540 0.84 +0.3
3,358,125 - RR42_RS15540 0.84 -1.0
3,358,181 + RR42_RS15540 0.88 -0.6
3,358,181 + RR42_RS15540 0.88 +0.5
3,358,385 + -0.2
3,358,385 + -0.2
3,358,385 + +0.1
3,358,386 - +0.7
3,358,386 - -0.3
3,358,487 - RR42_RS15545 0.17 +0.4
3,358,500 + RR42_RS15545 0.20 -1.3
3,358,501 - RR42_RS15545 0.20 +0.3
3,358,501 - RR42_RS15545 0.20 -0.5
3,358,519 + RR42_RS15545 0.24 +1.8
3,358,520 - RR42_RS15545 0.24 +0.1
3,358,520 - RR42_RS15545 0.24 +0.8
3,358,520 - RR42_RS15545 0.24 -1.5
3,358,520 - RR42_RS15545 0.24 +0.2
3,359,093 - RR42_RS15550 0.39 +0.3
3,359,255 + RR42_RS15550 0.67 +0.3
3,359,255 + RR42_RS15550 0.67 -1.6
3,359,255 + RR42_RS15550 0.67 +0.1
3,359,256 - RR42_RS15550 0.68 -0.2
3,359,256 - RR42_RS15550 0.68 -0.7
3,359,256 - RR42_RS15550 0.68 +0.4

Or see this region's nucleotide sequence